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RecruitingNCT03407859Updated Mar 15, 2024

Sequential Treatment With CD20/CD22/CD10-CART After CD19-CART Treatment Base on MRD in Relapsed/Refractory B-ALL

An Early Phase 1 interventional study of Sequential Treatment With different CART in Therapy Related Leukemia, sponsored by Zhujiang Hospital. Recruiting at 1 site in China. Open to participants aged 18 Years to 60 Years. Per ClinicalTrials.gov, last updated 2024-03-15.

Sponsored by Zhujiang Hospital · Early Phase 1, Interventional, and Treatment

Phase
Early Phase 1
Study type
Interventional
Enrollment
100
Allocation
Not applicable
Ages
18 Years to 60 Years
Sex
All
01

Study summary

CD19-negative B-ALL relapses after CD19 CAR T-cell treatment have occurred in some patients. CD20/CD22/CD10 is still expressed in CD19 negative B-ALL cells which means these CD molecules may become new targets in treatment of CD19-negative relapse of B-ALL. Thus sequential treatment with CD20/CD22/CD10-CART after CD19-CART treatment in relapsed/refractory B-ALL will kill and eliminate CD19 negative B-ALL cells and prolong the remission time.

Read the detailed description

B-cell acute lymphoblastic leukemia is the most common type of leukemia and the prognosis of relapsed/refractory B-ALL is poor. Chimeric Antigen Receptor-transduced T cell (CAR-T) therapy is one of revolutionary targeted immunotherapy. CD19 CAR-T is the most commonly used engineered T cell in B-ALL. The treatment effect is significant and far more than traditional therapy in relapsed/refractory B-ALL. However, the remission time after CD19 CAR-T infusion is short.CD19-positive and CD19-negative B-ALL relapses after CD19 CAR T-cell treatment have occurred in some patients The cause of relapse after CAR-T infusion is minimal residual disease (MRD) which will induce CD19 negative relapse. CD20/CD22/CD10 is still expressed in CD19 negative B-ALL cells which means these CD molecules may become new targets in treatment of CD19 negative relapse of B-ALL. Thus sequential treatment with CD20/CD22/CD10-CART after CD19-CART treatment in relapsed/refractory B-ALL will kill and eliminate CD19 negative B-ALL cells and prolong the remission time.

02

Conditions studied

  • Therapy Related Leukemia

Keywords

  • CD19-CART
  • CD20-CART
  • CD10-CART
  • CD22-CART
  • Relapsed/Refractory B-ALL
  • MRD
  • CD70-CART
03

Who can participate

Ages eligible
18 Years to 60 Years
Sexes eligible
All
Accepts healthy volunteers
No

Inclusion criteria

  1. Relapsed/Refractory B-ALL patients
  2. Did not achieve complete remission after 2 times of standard plan chemotherapy
  3. Relapsed after first induction chemotherapy
  4. Did not response to chemotherapy before HSCT or relapsed after HSCT
  5. Cannot receive allo-HSCT or refuse to receive allo-HSCT
  6. Cell phenotype is CD19 and CD20/CD22/CD10/CD70 positive (single or combined)
  7. Estimated survival time is more than 3 months in leukemia
  8. Volunteered for this clinical trail and signed a consent form

Exclusion criteria

Exclusion Criteria:

  1. MRD was negative while the cell phenotype was CD19 expressed
  2. Patients with severe insufficient cardiac, pulmonary and hepatorenal functions
  3. Patients with severe mental illness, neurological disease or infectious disease
  4. Patients with GVHD was taking immunosuppressants
  5. Pregnant or lactating women
  6. Patients have received other genetic therapy products
  7. Transfection efficiency was less than 30%
  8. Any situation may do harm to the subjects or interfere the results
04

Study design

Phase
Early Phase 1
Primary purpose
Treatment
Allocation
Not applicable
Intervention model
Sequential assignment
Masking
None (open label)
Enrollment
100 participants (estimated)

Study arms

  • Experimental
    Sequential therapy with different CART

    Sequential therapy With different CART including one kind of CD20/CD22/CD10-CART After CD19-CART therapy in CD19-negative relapse ALL patients, subjects will receive 1-5 x 10\^6/Kg transduced CAR T cells at one time.

    Biological: Sequential Treatment With different CART

Interventions

  • BiologicalSequential Treatment With different CART

    Sequential Treatment With CD20/CD22/CD10-CART After CD19-CART Treatment in Relapsed/Refractory B-ALL

05

What researchers measure

Primary outcomes

  1. Adverse events that Are related to treatment

    Determine the toxicity profile of the CD19-targeted and CD20/CD22/CD10-targeted CAR-T cells with Common Toxicity Criteria for Adverse Effects (CTCAE) version 4.0.

    Time frame: 2 years

Secondary outcomes

  1. Estimate 2 year overall survival(OS) after infusion of CD19-CART and sequential treatment

    To estimate 2 year overall survival(OS) after CD19-CART infusion and sequential treatment with Relapsed/Refractory B-ALL

    Time frame: 2 years

  2. Estimate relapse rate after infusion of CD19-CART and sequential treatment

    To estimate relapse rate after CD19-CART infusion and sequential treatment with Relapsed/Refractory B-ALL

    Time frame: 4 years

  3. Estimate 2 year progression free survival after infusion of CD19-CART and sequential treatment

    To estimate 2 year progression free survival (PFS) after CD19-CART infusion and sequential treatment with Relapsed/Refractory B-ALL

    Time frame: 2 years

06

Study locations

1 of 1 sites recruiting
  • Southern Medical University Zhujiang Hospital
    Guangdong, Guangdong 510000, China
    • Yuhua Li, PhD · Contact
    Recruiting
07

References and documents

Individual participant data

Plan to share: No

No publications or documents are linked to this record.

08

Registry details

Key details

Study ID
NCT03407859
Lead sponsor
Zhujiang Hospital
Collaborators
Nanfang Hospital, Southern Medical University
Responsible party
Sponsor
First posted
Jan 23, 2018
Start date
Jan 18, 2016
Primary completion
Dec 31, 2024 (estimated)
Completion
Mar 31, 2025 (estimated)
Last update
Mar 15, 2024

Study contacts

Yuhua Li, Ph.D
Contact
liyuhua2011gz@163.com
86-20-61643188
Sanfang Tu, Ph.D
Contact
doctortutu@163.com
86-20-62782322
Yanjie He, Ph.D
principal investigator · Zhujiang Hospital

Oversight

Data monitoring committee
Yes
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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