Perforated Collagen Membrane With Nanohydroxyapatite for Intrabony Defects

The study will be conducted on 12 individuals. The research esthetics committee of Faculty of Dentistry, Ain Shams University will review the proposal.

All patients will be informed about the nature of the study and will fine an informed consent.

I - Selection of the patients:

The patients will be selected from the outpatient clinic of Oral Medicine and Periodontology department, Faculty of Dentistry, Ain Shams University.

All the selected patients will be selected according to following criteria:

Inclusion criteria:

1. Patients free from any systemic diseases as evidenced by health using Burket's Oral Medicine health history questionnaire. (Glick, 2008)
2. Patients' age range from 25 to 50 years old.

3. Each patient should have at least two contralateral intrabony defects which fulfill the following criteria:

   (i) Probing depth ≥ 5 mm. (ii) Clinical attachment loss ≥ 5 mm. (iii) Radiographic evidence of vertical bone loss using periapical radiographs.

4. Patients are should be willing to participate in the study and should be able to return for the follow up visits.

Exclusion criteria:

1. Patients with history of periodontal surgery or medications including antibiotics and non-steroidal anti-inflammatory drugs for at least 3 months prior to the initiation of the study.
2. Pregnant females as well as breast feeding mothers.
3. Smokers.
4. Vulnerable groups as prisoners, mentally disabled, etc… All participants following initial clinical examination and periapical radiographs will be given detailed instructions in self-performed plaque control measures, including tooth brushing and dental flossing. All patients will receive full mouth scaling and root planing using ultra-sonic scalers and hand instruments under local anesthesia to minimize patient discomfort.

II- Study groups:

The selected sites will be randomly "double blinded study" divided into two groups in a split mouth design.

Group One:

The defects will be treated with open flap debridment followed by the placement of nanohydroxyapetite* combined with perforated collagen membrane**.

Group Two:

The defects will be treated with open flap debridment followed by the placement of nanohydroxyapetite combined with non perforated collagen membrane.

The mucoperiosteal flaps will be adapted and sutured and the patients will be given post-surgical instructions.

III- Periodontal evaluation:

Clinical Parameters:

The clinical parameters will be measured at one, three and six months postoperatively.

1) Plaque index (PI): Criteria of plaque index (PI) of (Silness and Loe, 1969) 0: No plaque in gingival area.

1. No plaque visible by the unaided eye, but plaque is made visible on the point of the probe after it has been moved across surface at entrance of gingival crevice.
2. Gingival area is covered with thin to moderately thick layer of plaque, deposit is visible to naked eye.
3. Heavy accumulation of plaque, the thickness of which fills out niche produced by gingival margin and tooth surface, inter dental area is stuffed with plaque.

2) Gingival index (GI): Criteria of the gingival index (GI) of (Loe, 1967) 0: Normal gingiva

1. Mild inflammation: slight change in color and slight edema; no bleeding on probing.
2. moderate inflammation: redness, edema and glazing; bleeding on probing.
3. Severe inflammation: marked redness and edema; ulceration; tendency to spontaneous bleeding.

3) Probing pocket depth (PPD) : Probing pocket depth (PPD) will be measured from the gingival margin to the base of periodontal pocket to the nearest mm (Caton, 1989).

Six readings will be recorded for each tooth: Mesiobuccal, mid-buccal, distobuccal, mesiolingual, mid-lingual and distolingual.

4) Clinical attachment level (CAL): Clinical attachment level will be measured from cementoenamel junction to the base of pocket to the nearest mm.

B- Radiographic parameters

Periapical radiographs and Cone beam computed tomography will be taken at base line and at six months after the placement of barrier membranes combined with nanohydroxyapatite and the following parameters will be measured:

The sites will be measured first on periapical radiographs and later on CBCT with the help of the ruler in the Image Tool software (University of Texas Health Science Centre, San Antonio, TX) based on the method proposed by Misch et al.

Three measurements will be performed for each site:

1. The height of the alveolar crest (AC): measured from the cementoenamel junction (CEJ) to the AC.
2. The depth of the defect, measured from the CEJ to the bottom of the defect.
3. The width of the defect, measured from the highest point of the AC to the dental root adjacent to the defect.

The axial slices will be used to verify the presence of combined bone defects and identify according to the classification of Goldman and Cohen.