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CompletedNCT03307980Updated Aug 11, 2026

Long-term Safety and Efficacy Study and Dose-Escalation Substudy of PF 06838435 in Individuals With Hemophilia B

A Phase 2 interventional study of PF-06838435 (formerly SPK-9001) in Hemophilia B, sponsored by Pfizer. Completed at 25 sites in 4 countries. Open to male participants aged 18 Years to 65 Years. Per ClinicalTrials.gov, last updated 2026-08-11.

Sponsored by Pfizer · Phase 2, Interventional, and Treatment

Phase
Phase 2
Study type
Interventional
Enrollment
21
Allocation
Not applicable
Ages
18 Years to 65 Years
Sex
Male
01

Study summary

Long-term safety and efficacy follow-up for participants with Hemophilia B who were previously treated in the C0371005 (formerly SPK-9001-101) study, and a dose-escalation sub-study evaluating safety, tolerability, and kinetics of a higher dose with long-term safety and efficacy follow-up. Participants in the substudy do not need to have participated in C0371005.

Read the detailed description

Evaluation of the long-term level of persistence and potential late or delayed adverse events associated with PF-06838435 (formerly SPK-9001), assessment of the durability of the transgene expression, and determination of the effects of PF-06838435 on clinical outcomes in individuals who have previously received a single administration of PF-06838435 in the C0371005 study. Amendment 2 of this study incorporates a dose-escalation substudy to evaluate the safety, tolerability, and kinetics of a single IV infusion of PF-06838435 at a higher dose than that used in the C0371005 study. The dose-escalation participants will also be followed for long-term safety and efficacy.

02

Conditions studied

  • Hemophilia B

Keywords

  • gene therapy
  • BeneGene LTE
  • hemophilia
  • SPK-9001
  • Factor IX
  • FIX
03

Who can participate

Ages eligible
18 Years to 65 Years
Sexes eligible
Male
Accepts healthy volunteers
No

This study is currently only enrolling into the dose-escalation substudy with subsequent long-term follow-up. The Eligibility Criteria for entry into the dose-escalation substudy is presented below:

Inclusion criteria

Inclusion Criteria:

  1. Able to provide informed consent and comply with requirements of the study
  2. Males age 18 to 65 years with confirmed diagnosis of hemophilia B (≤2 IU/dL or ≤2% endogenous factor IX)
  3. Received ≥50 exposure days to factor IX products
  4. No measurable factor IX inhibitor as assessed by the central laboratory and have no prior history of inhibitors to factor IX protein
  5. Agree to refrain from donating sperm and either abstain from intercourse or use reliable barrier contraception until 3 consecutive semen samples are negative for vector sequences

Exclusion criteria

Exclusion Criteria:

  1. Evidence of active hepatitis B or C
  2. Currently on antiviral therapy for hepatitis B or C
  3. Have significant underlying liver disease
  4. Serological evidence* of HIV-1 or HIV-2 with CD4 counts ≤200/mm3 (* participants who are HIV+ and stable with CD4 count >200/mm3 and undetectable viral load are eligible to enroll)
  5. Neutralizing antibody titers to the capsid portion of PF-06838435 above the established threshold
  6. Sensitivity to heparin or heparin induced thrombocytopenia; sensitivity to any of the study interventions, or components thereof, or drug or other allergy
  7. Previously dosed in a gene therapy research trial at any time or in an interventional clinical study within 3 months of screening visit
  8. Any concurrent clinically significant major disease or condition
  9. Unable or unwilling to comply with the study procedures
04

Study design

Phase
Phase 2
Primary purpose
Treatment
Allocation
Not applicable
Intervention model
Sequential assignment
Masking
None (open label)
Enrollment
21 participants (actual)

Study arms

  • Experimental
    PF-06838435 Dose-Escalation

    Single intravaneous infusion of PF-06838435. After 2 participants receive initial dose, data will be evaluated and a decision will be made to escalate or reduce the dose being evaluated, increase the number of participants receiving the dose, or stop dosing. Multiple iterations may be undertaken.

    Biological: PF-06838435 (formerly SPK-9001)

Interventions

  • BiologicalPF-06838435 (formerly SPK-9001)

    Gene Therapy: A novel, bioengineered adeno-associated viral vector carrying human factor IX variant

05

What researchers measure

Primary outcomes

  1. Incidence of PF-06838435 related adverse events

    Time frame: Baseline up to Year 6

Secondary outcomes

  1. Incidence of clinically significant changes from baseline

    Clinically significant changes in physical examination, vital signs, laboratory values. (to be reported as AEs, regardless of causality)

    Time frame: Baseline up to 52 weeks

  2. Incidence of protocol-defined medically important events

    Clinical thrombotic events, FIX inhibitor development as assessed by Nijmegen Bethesda assay, Hypersensitivity reaction (eg, bronchospasm and anaphylaxis), Hepatic malignancy, Study intervention-related elevated hepatic transaminases that fail to improve or resolve, Malignancy assessed as having reasonable possibility of being related to study intervention (to be reported as SAEs).

    Time frame: Baseline up to 52 weeks

  3. Immune response against AAV capsid protein and hFIX transgene

    Positive immune response based on peripheral blood mononuclear cell (PBMC) results by interferon gamma enzyme-linked immunospot assay (ELISPOT).

    Time frame: Baseline up to 52 weeks

  4. Coagulation Clotting Assay for FIX activity levels

    Coagulation Clotting assays to assess FIX activity levels (percent of normal)

    Time frame: Baseline up to Year 6

  5. Mean and standard deviation of vector-derived FIX Activity levels

    Mean and standard deviation of peak and steady-state FIX Activity

    Time frame: Baseline up to 52 weeks

  6. Mean and standard deviation of FIX Antigen levels

    Mean and standard deviation of FIX Antigen levels

    Time frame: Baseline up to 52 weeks

  7. Annualized bleeding rate (ABR)

    ABR (not including those for surgery)

    Time frame: Baseline up to Year 6

  8. Annualized (factor FIX) infusion rate

    AIR (not including those for surgery)

    Time frame: Baseline up to Year 6

  9. Total factor consumption (IU)

    total quantity of factor infused annually (not including those for surgery) as recorded on the infusion log

    Time frame: Baseline up to Year 6

  10. Total number of bleeding events

    spontaneous and traumatic

    Time frame: Baseline up to Year 6

  11. Haem-A-QoL

    Quality-of-life (QoL) assessment

    Time frame: Baseline up to Year 6

  12. EQ-5D-5L

    Quality-of-life (QoL) assessment

    Time frame: Baseline up to Year 6

  13. Brief Pain Inventory

    Quality-of-life (QoL) assessment

    Time frame: Year 2 up to Year 6

  14. McGill Pain Questionnaire

    Quality-of-life (QoL) assessment

    Time frame: Baseline up to 52 weeks

06

Study locations

25 sites
  • UC Davis Comprehensive Cancer Center
    Sacramento, California 95817, United States
  • UC Davis Ellison Ambulatory Care Clinic
    Sacramento, California 95817, United States
  • UC Davis Medical Center department of Radiology
    Sacramento, California 95817, United States
  • UC Davis Medical Center
    Sacramento, California 95817, United States
  • UC Davis Midtown Cancer Center
    Sacramento, California 95817, United States
  • UC DavisHealth Main Hospital
    Sacramento, California 95817, United States
  • LA Center for Bleeding and Clotting Disorders - Metairie
    Metairie, Louisiana 70001, United States
  • Tulane Lakeside Hospital
    Metairie, Louisiana 70001, United States
  • LA Center for Bleeding and Clotting Disorders
    New Orleans, Louisiana 70112, United States
  • Tulane University Clinical Translational Unit
    New Orleans, Louisiana 70112, United States
  • Tulane University Hospitals and Clinic
    New Orleans, Louisiana 70112, United States
  • Tulane University School of Medicine
    New Orleans, Louisiana 70112, United States
  • University Medical Center New Orleans
    New Orleans, Louisiana 70112, United States
  • Louisiana Center for Advanced Medicine
    Slidell, Louisiana 70461, United States
  • Mississippi Center for Advanced Medicine
    Madison, Mississippi 39110, United States
  • Weill Cornell Medicine - New York Presbyterian Hospital
    New York, New York 10065, United States
  • The Children's Hospital of Philadelphia
    Philadelphia, Pennsylvania 19104, United States
  • Royal Prince Alfred Hospital
    Camperdown, New South Wales 2050, Australia
  • McMaster University Medical Centre - Hamilton Health Sciences
    Hamilton, Ontario L8N 3Z5, Canada
  • McMaster University
    Hamilton, Ontario L8S 3L8, Canada
  • The Research Institute of the McGill University Health Centre
    Montreal, Quebec H3H 2R9, Canada
  • McGill University Health Center - Research Institute
    Montreal, Quebec H4A 3J1, Canada
  • Istanbul Universitesi Onkoloji Enstitusu Çocuk Hematoloji Onkoloji Bilim Dali
    Istanbul, 34093, Turkey (Türkiye)
  • Istanbul University Clinical Trials Excellence Center
    Istanbul, 34452, Turkey (Türkiye)
  • Ege Universitesi Tip Fakultesi Cocuk Sagligi ve Hastaliklari Anabilim Dali
    Izmir, 35100, Turkey (Türkiye)
07

References and documents

Publications

  • Samelson-Jones BJ, Rasko JEJ, Ducore JM, McGuinn CE, George LA, von Mackensen S, Borgonuovo G, Agathon D, Smith L, Wilcox LJ, Biondo F, Plonski F. Safety, efficacy, and patient-reported outcomes 6 years after fidanacogene elaparvovec in adults with hemophilia B. Blood Adv. 2026 May 26;10(10):3517-3526. doi: 10.1182/bloodadvances.2025019174. PubMed 41734390 ↗
  • Wojciechowski J, Gaitonde P, Hughes JH, Ravva P. Population Modeling of Factor IX Activity Following Administration of Fidanacogene Elaparvovec Gene Therapy in Participants with Hemophilia B. Clin Pharmacokinet. 2025 Oct;64(10):1531-1548. doi: 10.1007/s40262-025-01535-y. Epub 2025 Aug 1. PubMed 40750723 ↗
  • Rasko JEJ, Samelson-Jones BJ, George LA, Giermasz A, Ducore JM, Teitel JM, McGuinn CE, High KA, de Jong YP, Chhabra A, O'Brien A, Smith LM, Winburn I, Rupon J. Fidanacogene Elaparvovec for Hemophilia B - A Multiyear Follow-up Study. N Engl J Med. 2025 Apr 17;392(15):1508-1517. doi: 10.1056/NEJMoa2307159. PubMed 40239068 ↗

Individual participant data

Plan to share: Yes — Pfizer will provide access to individual de-identified participant data and related study documents (e.g. protocol, Statistical Analysis Plan (SAP), Clinical Study Report (CSR)) upon request from qualified researchers, and subject to certain criteria, conditions, and exceptions. Further details on Pfizer's data sharing criteria and process for requesting access can be found at: https://www.pfizer.com/science/clinical\_trials/trial\_data\_and\_results/data\_requests.

08

Registry details

Key details

Study ID
NCT03307980
Lead sponsor
Pfizer
Responsible party
Sponsor
First posted
Oct 12, 2017
Start date
Jun 22, 2017
Primary completion
Jul 23, 2026
Completion
Jul 23, 2026
Last update
Aug 11, 2026

Study contacts

Pfizer CT.gov Call Center
study director · Pfizer

Oversight

Data monitoring committee
Yes
FDA-regulated drug
Yes
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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This study is completed, as verified in Aug 2026. You cannot join it, but the record below documents what was studied.

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