An interventional study of Blood sampling in Cardiac Insufficiency, sponsored by University Hospital, Toulouse. Completed at 1 site in France. Open to participants aged 18 Years and older. Per ClinicalTrials.gov, last updated 2019-08-20.
Sponsored by University Hospital, Toulouse · Not applicable, Interventional, and Basic science
To compare in vivo platelet and leukocyte activation and phenotype before and after Left ventricular assist device (LAVD) implantation
The present study plan to investigate platelet activation in vivo using a variety of biomarkers previously identified as sensitive to high shear stress in patients with severe aortic valve stenosis before and after trans arterial valve replacement. As one of the best biomarker is a high degree of platelet-leukocyte interactions, the study will study in parallel leukocytes activation/differentiation. These parameters will be compared at 3 time-points, before and after LAVD implantation in ambulatory patients.
Exclusion Criteria:
Ten patients before and after LAVD implantation had blood sampling
Other: Blood sampling
Three venous blood sampling before the left ventricular assist device implantation and 1 and 2 months after implantation in ambulatory patients.
Platelet activation
To compare platelet activation in vivo, using a composite criteria determinated by variety of biomarkers of activation analysed by flow cytometry and enzyme-linked immunosorbent assay (ELISA)
Time frame: Month 2 after LAVD implantation
Level of circulating platelet-leukocyte conjugate
To determine the level of circulating platelet-leukocyte conjugate by flow cytometry
Time frame: Month 2 after LAVD implantation
Changes in leukocyte phenotype
To determine the leukocyte phenotype which is a composite criteria analysed by flow cytometry, ELISA and lipidaemic analysis
Time frame: Month 2 after LAVD implantation
Plan to share: No
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University Hospital, Toulouse