CClinicalTrials.gg
CompletedNCT03284645VADICTUpdated Oct 14, 2020

Viral and Antiretroviral Dynamics in HIV-1 Mother-to-Child Transmission Fluids

An observational study in Human Immunodeficiency Virus Infection, sponsored by Obafemi Awolowo University. Completed at 4 sites in Nigeria. Open to female participants aged 18 Years and older. Per ClinicalTrials.gov, last updated 2020-10-14.

Sponsored by Obafemi Awolowo University · Observational

Study type
Observational
Model
Cohort
Time perspective
Prospective
Enrollment
194
Ages
18 Years and older
Sex
Female
01

Study summary

More than 150,000 babies became infected with HIV in 2015 alone. When HIV drugs are started before or early in pregnancy, HIV positive women can give birth to HIV negative baby. This is possible because HIV drugs can reduce the amount of the virus in the body to the extent that they become undetectable by the time of delivery and during the breastfeeding period. However, some women do not start taking these drugs on time because they become infected during pregnancy or lactation. This leads to detectable virus at the time of delivery and puts the baby at risk of becoming infected. Also, the amounts of HIV drugs in the body have to be at certain levels for them to work effectively. But findings from some research have recently showed that pregnancy increases the rate at which the body removes some HIV drugs used to prevent the transfer of HIV from mother to child. While this may not cause any problem in women with no detectable virus before pregnancy, it may affect the rate at which the HIV virus is removed from the body in those starting treatment late and may put the baby at risk. This project will investigate whether the changes in drug exposure caused by pregnancy or other factors have any effect on the rate at which the HIV virus is removed from the body. HIV positive pregnant women and those who recently delivered will be recruited from different hospitals and follow up will be until breastfeeding ends. The investigators will not be involved in treatment decisions and the primary care provider will be responsible for prescribing antiretroviral regimen based on current guidelines. Samples will be collected to measure levels of the virus and the drugs in three fluids that transfer the virus to the baby: blood, genital fluid, and breastmilk. The HIV status of the babies will be monitored until they stop breastfeeding.

02

Conditions studied

  • Human Immunodeficiency Virus Infection
03

Who can participate

Ages eligible
18 Years and older
Sexes eligible
Female
Accepts healthy volunteers
No
Sampling method
Non-probability sample

Study population

This is cohort study of HIV positive pregnant or recently postpartum women receiving WHO recommended first-line ART regimen. Pregnant women and nursing mothers initiating ART \< 4 months before delivery (n = 60) and \< 6 weeks postpartum (n = 60), respectively, and a comparison group of pregnant women who initiated ART ≥ 4 months before delivery (n = 120) will be recruited from four hospitals providing PMTCT services in Nigeria.

Inclusion criteria

  • ≥ 18 years old
  • Planned exclusive breastfeeding until 6 months of age
  • Able to understand study information and comply with follow-up schedule

Exclusion criteria

Exclusion Criteria:

  • Severe maternal or infant illness
  • Planned exclusive formula feeding
  • Taking medication with known or uncertain interaction with study drugs
04

Study design

Observational model
Cohort
Time perspective
Prospective
Enrollment
194 participants (actual)
Patient registry
No

Groups and cohorts

  • ART Before or Early in Pregnancy

    HIV positive pregnant women who started antiretroviral therapy (ART) before or early in pregnancy for prevention of mother-to-child transmission of HIV and for their own health.

    Drug: Tenofovir Disoproxil Fumarate (TDF) 300 mg + Lamivudine (3TC) 300 mg + Efavirenz (EFV) 600 mg · Drug: Abacavir (ABC) 600 mg + Lamivudine (3TC) 300 mg + Efavirenz (EFV) 600 mg · Drug: Zidovudine (AZT) 300 mg + Lamivudine (3TC) 150 mg twice daily + Efavirenz (EFV) 600 mg once daily

  • ART Started Third Trimester

    HIV positive pregnant women starting antiretroviral therapy (ART) during the third trimester of pregnancy for prevention of mother-to-child transmission of HIV and for their own health.

    Drug: Tenofovir Disoproxil Fumarate (TDF) 300 mg + Lamivudine (3TC) 300 mg + Efavirenz (EFV) 600 mg · Drug: Abacavir (ABC) 600 mg + Lamivudine (3TC) 300 mg + Efavirenz (EFV) 600 mg · Drug: Zidovudine (AZT) 300 mg + Lamivudine (3TC) 150 mg twice daily + Efavirenz (EFV) 600 mg once daily

  • ART Started Postpartum

    HIV positive women starting antiretroviral therapy (ART) after delivery for prevention of mother-to-child transmission of HIV and for their own health.

    Drug: Tenofovir Disoproxil Fumarate (TDF) 300 mg + Lamivudine (3TC) 300 mg + Efavirenz (EFV) 600 mg · Drug: Abacavir (ABC) 600 mg + Lamivudine (3TC) 300 mg + Efavirenz (EFV) 600 mg · Drug: Zidovudine (AZT) 300 mg + Lamivudine (3TC) 150 mg twice daily + Efavirenz (EFV) 600 mg once daily

Interventions

  • DrugTenofovir Disoproxil Fumarate (TDF) 300 mg + Lamivudine (3TC) 300 mg + Efavirenz (EFV) 600 mg

    Fixed-dose combination of 300 mg TDF, 300 mg 3TC and 600 mg EFV taken once daily.

  • DrugAbacavir (ABC) 600 mg + Lamivudine (3TC) 300 mg + Efavirenz (EFV) 600 mg

    Fixed-dose combination of 600 mg ABC, 300 mg 3TC and 600 mg EFV taken once daily.

  • DrugZidovudine (AZT) 300 mg + Lamivudine (3TC) 150 mg twice daily + Efavirenz (EFV) 600 mg once daily

    Fixed-dose combination of 300 mg AZT and 150 mg 3TC taken twice daily, plus 600 mg EFV taken once daily.

05

What researchers measure

Primary outcomes

  1. Polymorphisms in antiretrovirals disposition genes

    Time frame: At study enrolment

  2. Minimum plasma drug concentration (Cmin)

    Time frame: At 2-3 months before delivery and at 10-12 weeks postpartum

  3. Maximum plasma drug concentration (Cmax)

    Time frame: At 2-3 months before delivery and at 10-12 weeks postpartum

  4. Area under the concentration-time curve (AUC)

    Time frame: At 2-3 months before delivery and at 10-12 weeks postpartum

  5. Clearance over systemic availability (Cl/F)

    Time frame: At 2-3 months before delivery and at 10-12 weeks postpartum

  6. HIV-1 viral load (RNA & DNA) in plasma

    Time frame: Through study completion (1-2 monthly)

  7. HIV-1 viral load (RNA & DNA) in breastmilk

    Time frame: From 6 weeks postpartum through study completion (1-2 monthly)

  8. HIV-1 viral load (RNA & DNA) in CVF

    Time frame: From week 28 to delivery (monthly)

06

Study locations

4 sites
  • St. Monica's Hospital
    Adikpo, Benue State, Nigeria
  • St. Thomas' Hospital
    Ihugh, Benue State, Nigeria
  • Bishop Murray Medical Centre
    Makurdi, Benue State, Nigeria
  • Federal Medical Centre
    Makurdi, Benue State, Nigeria
07

References and documents

Publications

  • Olagunju A, Anweh D, Okafor O et al. Viral and antiretroviral dynamics in HIV mother-to-child transmission fluids (VADICT) - Protocol and data analysis plan for a cohort study [version 1; referees: awaiting peer review]. Wellcome Open Res 2019, 4:34

Individual participant data

Plan to share: No

08

Registry details

Key details

Study ID
NCT03284645
Lead sponsor
Obafemi Awolowo University
Collaborators
Federal Medical Centre, Makurdi, University of Liverpool, University of California, San Diego, London School of Hygiene and Tropical Medicine
Responsible party
Sponsor
First posted
Sep 15, 2017
Start date
Dec 22, 2017
Primary completion
Dec 31, 2019
Completion
Sep 17, 2020
Last update
Oct 14, 2020

Study contacts

Adeniyi Olagunju, PhD
principal investigator · Obafemi Awolowo University, Nigeria

Oversight

Data monitoring committee
Yes
FDA-regulated drug
Yes
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

Not currently enrolling

This study is completed, as verified in Oct 2020. You cannot join it, but the record below documents what was studied.

Follow this study

Get an email when the registry record changes — status, dates, results — or when someone posts here.

Sign in to follow

Discussion

Questions and observations about this study, from anyone following it. Not medical advice, and not a channel to the study team — their contact details are on the registry record.

Sign in to join the discussion. Reading takes no account; posting does. You choose a display name, and a pseudonym is the default.

Nothing here yet. If you are running this trial, taking part in it, or weighing whether to, this is the place to say so.

Start the discussion