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CompletedNCT03272126Updated May 24, 2018

Importance of Dosing Regimen for the Effect of Vitamin D Supplementation

A Phase 3 interventional study of Vitamin D and Placebo in Hypovitaminosis D, sponsored by University of Tromso. Completed at 1 site in Norway. Open to participants aged 20 Years to 70 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2018-05-24.

Sponsored by University of Tromso · Phase 3, Interventional, and Prevention

Phase
Phase 3
Study type
Interventional
Enrollment
61
Allocation
Randomized
Ages
20 Years to 70 Years
Sex
All
01

Study summary

Vitamin D is a hormone with effects not only on the skeleton, but on most tissues in the body. Lack of vitamin D is associated with cardio-vascular disease (CVD), type 2 diabetes, cancer, infectious and immunological diseases, as well as risk factors for these diseases. However, intervention studies with vitamin D have been inconclusive regarding diseases and risk factors. This could be due to inclusion of subjects already vitamin D sufficient, and short and underpowered studies. In addition, there are indications that the dosing regimens may be important, so that daily doses with vitamin D are more efficient than intermittent doses, which so far have been generally used. This could be related to the concentration of circulating and thereby intracellular vitamin D concentrations, which probably is dependent on daily vitamin D doses. This will be tested in the present study where 60 subjects will be randomized to vitamin D 160 000 once, vitamin D 4000 IU/day, or placebo for four weeks. The primary endpoints will be effects on serum hepcidin and plasma cathelicidin after 4 weeks, with effects on serum PTH, RNA expression and microRNA in peripheral blood, telomerase activity in peripheral blood mononuclear cells and the ration between serum 1,25(OH)2D and 24,25(OH)2D as secondary endpoints.

02

Conditions studied

  • Hypovitaminosis D

Keywords

  • vitamin D
03

Who can participate

Ages eligible
20 Years to 70 Years
Sexes eligible
All
Accepts healthy volunteers
Yes

Eligibility criteria

Inclusion Criteria:- Males and females 20 - 70 years

  • 20 kg/m2 > BMI \< 35 kg/m2
  • systolic BP \< 175 mmHg, diastolic BP \< 105 mmHg
  • Serum 25(OH)D level \< 50 nmol/L. Serum 25(OH)D \< 50 nmol/L is considered as vitamin D deficiency, and accordingly, effects of vitamin D supplementation will be easier to detect than if the included subjects were vitamin D sufficient.
  • Hgb, SR, CRP, creatinine \< 130 umol/L in males, \< 120 umol/L in females, calcium, FT4 and TSH within the normal reference range; ASAT \< 90 mU/L ALAT \< 140 U/L, HbA1c \< 6.6 %
  • The subjects must agree not to take any vitamin D supplementation, including cod liver oil or "mølje", use solarium or go on sunny vacation during the intervention period.

Exclusion Criteria:

  • subjects allergic to peanuts
  • subjects with primary hyperparathyroidism
  • granulomatous diseases (sarcoidosis, tuberculosis, Wegner's granulomatosis)
  • diabetes
  • renal stones the last five years
  • subjects seriously ill (or with chronic disease) and unfit for participation in the study (as judged by one of the study doctors)
  • subjects using vitamin D supplements exceeding 800 IU per day or active vitamin D drugs (Rocaltrol or Etalpha)
  • pregnancy
04

Study design

Phase
Phase 3
Primary purpose
Prevention
Allocation
Randomized
Intervention model
Parallel assignment
Masking
Quadruple (Participant, Care provider, Investigator, Outcomes assessor)
Enrollment
61 participants (actual)

Study arms

  • Experimental
    vitamin D bolus

    vitamin D 160 000 IU given as bolus

    Drug: Vitamin D

  • Experimental
    vitamin D daily

    vitamin D 4000 IU daily for 28 days

    Drug: Vitamin D

  • Placebo comparator
    placebo

    identical looking as vitamin d

    Drug: Placebo

Interventions

  • DrugVitamin D

    vitamin D given as bolus versus daily dosing

  • DrugPlacebo

    identical looking as vitamin D

05

What researchers measure

Primary outcomes

  1. Hepcidin change

    Difference between vitamin D given as a bolus 160 000IU vs 4000 IU regarding change in serum hepcidin

    Time frame: 4 weeks

Secondary outcomes

  1. Difference in serum PTH after 1 month

    Difference between vitamin D given as a bolus 160 000IU vs 4000 IU regarding change in serum PTH, cathelicidin, RNA expression and micro RNA in peripheral blood, telomerase activity and the ratio between 1,25(OH)2D and 24,25(OH)2D, after 1 month

    Time frame: 4 weeks

  2. Difference in micro RNA Expression after 1 month

    Difference between vitamin D given as a bolus 160 000IU vs 4000 IU

    Time frame: 4 weeks

  3. Difference in serum cathelicidin after 1 month

    Difference between vitamin D given as a bolus 160 000IU vs 4000 IU

    Time frame: 4 weeks

  4. Difference in RNA Expression after 1 month

    Difference between vitamin D given as a bolus 160 000IU vs 4000 IU

    Time frame: 4 weeks

  5. Difference in telomerase Activity after 1 month

    Difference between vitamin D given as a bolus 160 000IU vs 4000 IU

    Time frame: 4 weeks

  6. Difference in ratio between 1,24(OH)2D and 24,24(OH)2D after 1 month

    Difference between vitamin D given as a bolus 160 000IU vs 4000 IU

    Time frame: 4 weeks

06

Study locations

1 site
  • University Hospital of North Norway
    Tromsø, 9038, Norway
07

References and documents

Individual participant data

Plan to share: No

No publications or documents are linked to this record.

08

Registry details

Key details

Study ID
NCT03272126
Lead sponsor
University of Tromso
Responsible party
Sponsor
First posted
Sep 5, 2017
Start date
Dec 1, 2017
Primary completion
Apr 1, 2018
Completion
May 1, 2018
Last update
May 24, 2018

Study contacts

rolf jorde, MD PhD
principal investigator · UiT, Tromsø

Oversight

Data monitoring committee
No
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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