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CompletedNCT02707393Updated Apr 15, 2021

Allogeneic Stem Cell Transplantation for Children With CML

A Phase 2/3 interventional study of Fludarabine and Thiotepa in Chronic Myeloid Leukemia, sponsored by St. Anna Kinderkrebsforschung. Completed at 4 sites in 3 countries. Open to participants aged 1 Year to 18 Years. Per ClinicalTrials.gov, last updated 2021-04-15.

Sponsored by St. Anna Kinderkrebsforschung · Phase 2/3, Interventional, and Treatment

Phase
Phase 2/3
Study type
Interventional
Enrollment
13
Allocation
Not applicable
Ages
1 Year to 18 Years
Sex
All
01

Study summary

In children and adolescents with chronic myeloid leukaemia (CML) stem cell transplantation (SCT) may be a valid alternative to the life-long treatment with tyrosinkinase inhibitors (TKI). This trial aims to evaluate the use of a reduced intensity conditioning regimen (RIC), consisting of fludarabine, melphalan and thiotepa in order to minimize transplant related mortality and toxic late effects. Strict post-transplant monitoring and reintroduction of TKI as well as donor lymphocyte infusions (DLI) in case of relevant residual disease are part of the protocol.

Read the detailed description

Chronic myeloid leukaemia (CML) is a rare disease in children with an incidence of 3-5% of all paediatric leukaemias. Since the introduction of tyrosinkinase inhibitors (TKI) stem cell transplantation (SCT) is no longer the first choice treatment for patients with early phase CML.

However life-long treatment with TKI may not be feasable in several cases due to side effects such as growth retardation, non-compliance and resistance. This protocol evaluates the feasibility of SCT following a reduced intensity conditioning regimen (RIC) consisting of fludarabine, melphalan, thiotepa and thymoglobuline (ATG). Matched siblings and matched unrelated donors are permitted for stem cell donation. In case of unrelated donors tissue typing has to be done by high resolution molecular typing. Donors with 10/10 or 9/10 identical allels in the human leukocyte antigen (HLA) system are accepted. Preferred stem cell source is bone marrow but peripheral blood stem cells and umbilical cord blood are also allowed. Graft-versus-Host-Disease (GvHD)-prophylaxis is achieved with cyclosporine A and mycophenolate mofetil.

Monitoring of the breakpoint cluster region - Abelson (BCR/ABL) rearrangement is performed monthly in the first year after SCT. In case of BCR/ABL positivity TKI are given in the first year after SCT. Followed by donor lymphocyte infusions (DLI) later on if BCR/ABL positivity persists.

02

Conditions studied

  • Chronic Myeloid Leukemia

Keywords

  • stem cell transplantation
  • pediatrics
03

Who can participate

Ages eligible
1 Year to 18 Years
Sexes eligible
All
Accepts healthy volunteers
No

Inclusion criteria

  • children and adolescents with BCR/ABL positive CML in chronic phase, who are eligible for allogeneic stem cell transplantation, irrespective of the previous treatment strategy
  • availability of a HLA matched sibling donor (MSD), a matched family donor, a matched unrelated donor or a matched unrelated cord blood (MD)
  • informed consent

Exclusion criteria

Exclusion Criteria:

  • unavailability of MSD or MD
  • patients in accelerated phase or blast crisis
  • pregnancy
  • previous autologous or allogeneic SCT
  • no informed consent
04

Study design

Phase
Phase 2 / Phase 3
Primary purpose
Treatment
Allocation
Not applicable
Intervention model
Single group
Masking
None (open label)
Enrollment
13 participants (actual)

Study arms

  • Experimental
    single arm

    Fludarabine intravenous - daily dose: 40mg/sqm on day -7, -6, -5, -4; Thiotepa intravenous - daily dose: 2 x 5mg/kg on day -3; Melphalan intravenous - daily dose: 140/mg/sqm on day - 2; ATG intravenous - dose according to local standards on day -3, -2, -1; bone marrow or peripheral blood stem cells of an HLA identical sibling or matched unrelated donor on day 0; GvHD propyhlaxis with Mycophenolate Mofetil and Cyclosporine A

    Drug: Fludarabine · Drug: Thiotepa · Drug: Melphalan · Drug: ATG · Drug: Cyclosporine A · Drug: Mycophenolate mofetil · Biological: bone marrow or peripheral blood stem cells

Interventions

  • DrugFludarabine

    infusion

    Also known as: Fludara

  • DrugThiotepa

    infusion

    Also known as: Thioplex, TESPA

  • DrugMelphalan

    infusion

    Also known as: Alkeran

  • DrugATG

    infusion

    Also known as: Thymoglobulin

  • DrugCyclosporine A

    infusion, orally if possible

    Also known as: Sandimmune

  • DrugMycophenolate mofetil

    infusion

    Also known as: CellCept

  • Biologicalbone marrow or peripheral blood stem cells

    infusion

05

What researchers measure

Primary outcomes

  1. transplant related mortality

    Time frame: one year

Secondary outcomes

  1. overall survival

    Time frame: five years

  2. event free survival

    Time frame: five years

06

Study locations

4 sites
  • Universitätsklinik für Kinder- und Jugendheilkunde
    Graz, 8036, Austria
  • St. Anna Kinderspital
    Wien, 1050, Austria
  • Hospital Motol, Department of Pediatric Hematology and Oncology, BMT Unit
    Praha, 15006, Czechia
  • Clinica Pediatrica
    Monza, 20052, Italy
07

References and documents

Individual participant data

Plan to share: Undecided

No publications or documents are linked to this record.

08

Registry details

Key details

Study ID
NCT02707393
Lead sponsor
St. Anna Kinderkrebsforschung
Responsible party
Sponsor
First posted
Mar 14, 2016
Start date
Apr 30, 2009
Primary completion
Dec 1, 2020
Completion
Dec 1, 2020
Last update
Apr 15, 2021

Study contacts

Susanne Matthes, MD
study chair · St. Anna Kinderspital

Oversight

Data monitoring committee
Yes
View the source record on ClinicalTrials.gov ↗

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