An interventional study of 2-15N glutamine and 15N2 arginine and 15N proline in Healthy, sponsored by The Hospital for Sick Children. Completed at 1 site in Canada. Open to male participants aged 18 Years to 55 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2018-01-09.
Sponsored by The Hospital for Sick Children · Not applicable, Interventional, and Diagnostic
Arginine synthesis in mammals is a complex process involving at least 8 enzymes and intracellular and interorgan transfer of substrates.
The investigators have shown in our neonatal studies that there is no conversion of glutamate to arginine but proline label appears in ornithine, citrulline and arginine. It is clear therefore that arginine is synthesized in human neonates from proline.
In adults the source of the carbon backbone is less clear.
Through a series of test in healthy adults using tandem mass spectrometry with carbon labeled GLN and nitrogen labeled PRO to determine the dietary source for the carbon chain of ORN, CIT and ARG. Additionally, using Nitrogen labeled GLN, we will identify the source of the nitrogen atoms in these amino acids.
Using guanidino - 15N2 arginine, 1-13C Glutamine, 2-15N Glutamine and 15N Proline we will be able to:
The Hospital for Sick Children is the lead sponsor of 568 studies on the registry; 81 are open to participants now.
Of its 7 completed or terminated interventional studies of FDA-regulated products, 0 (0%) have results posted.
Counted across the registry records on this site, refreshed daily.
Exclusion Criteria:
To determine the contributions make by glutamine to the provision of nitrogen for ornithine, citrulline and arginine syntesis in adults.
Other: 2-15N glutamine
administration of 15N2 arginine \&15N proline to measure arginine and proline flux
Other: 15N2 arginine and 15N proline
to determine to contribution of glutamine to the carbon skeleton of ornithine, citrulline and arginine in adults.
Other: 1-13C glutamine
Glutamine with N15 isotope tracer
Arginine and Proline isotope tracers
Glutamine carbon isotope tracer
Blood Isotope Levels
Time frame: 8 hours
This study is completed, as verified in Jan 2018. You cannot join it, but the record below documents what was studied.
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The Hospital for Sick Children