An interventional study of Aspiration without flushing follicles with culture media and Follicular Flushing in Fertilization, sponsored by Weill Medical College of Cornell University. Completed at 1 site in United States. Open to female participants aged 18 Years to 45 Years. Per ClinicalTrials.gov, last updated 2019-01-14.
Sponsored by Weill Medical College of Cornell University · Not applicable, Interventional, and Treatment
Patients with infertility often undergo in vitro fertilization (IVF) to achieve a pregnancy, which involves ovarian stimulation, monitoring of follicular growth, oocyte retrieval, sperm insemination, embryo culture and embryo transfer.
The oocytes are removed during surgery by aspirating the follicles using a single lumen needle with an ultrasound to guide the procedure. There is some data that flushing the follicles with embryo culture media before aspiration using a double lumen needle increases the number of oocytes retrieved, particularly among poor responding patients for whom each additional oocyte recovered may substantially alter the outcome of that IVF cycle.
The objective of the research is to evaluate the effect of follicular flushing in poor responders on IVF cycle outcomes.
Patients with infertility often undergo in vitro fertilization (IVF) to achieve a pregnancy, which involves ovarian stimulation, monitoring of follicular growth, oocyte retrieval, sperm insemination, embryo culture and embryo transfer. The number of embryos obtained is dependent on the number of oocytes retrieved.
The objective of the research is to evaluate the effect of follicular flushing in poor responders on IVF cycle outcomes, including the number of oocytes retrieved, number of mature oocytes, fertilization rate, number of embryos transferred, implantation rate, miscarriage rate, live birth rate, operating room time, and luteal estradiol and progesterone levels.
The research entails a prospective, randomized, controlled trial of poor-responder patients who are undergoing IVF treatment at The Ronald O. Perelman and Claudia Cohen Center for Reproductive Medicine, defined as patients who have ≤ 4 follicles that are ≥ 12mm on the day of hCG trigger.
Patients identified as poor responders will be consented and randomized to follicular flushing versus aspiration without flushing. A 2-5mL flush using embryo culture media followed by aspiration of the follicle will be performed in the patients randomized to the flushing group, with subsequent flushes until an oocyte is identified or up to 4 flushes maximum.
Patients being enrolled in this research study will undergo IVF regardless of participation in this research study.
Randomization:
A series of randomized blocks of 2 will be generated for the study. This will provide assurance that after two patients are enrolled, there will be one patient assigned to each group- follicular flushing versus aspiration without flushing. This process of blocked randomization ensures an equal distribution between the two study arms of patients.
Exclusion Criteria:
Flushing follicles with embryo culture media prior to aspiration.
Other: Aspiration without flushing follicles with culture media · Other: Follicular Flushing
Aspiration without flushing follicles with culture media.
Flushing follicles with embryo culture media prior to aspiration.
FOLLICULAR FLUSHING ON CYCLE OUTCOMES IN POOR RESPONDERS
To evaluate the number of oocytes retrieved with follicular flushing in poor responders
Time frame: 2 weeks
FOLLICULAR FLUSHING ON CYCLE OUTCOMES IN POOR RESPONDERS
To evaluate: - # of embryos transferred
Time frame: 2 weeks
FOLLICULAR FLUSHING ON CYCLE OUTCOMES IN POOR RESPONDERS
Fertilization rates.
Time frame: 2 weeks
FOLLICULAR FLUSHING ON CYCLE OUTCOMES IN POOR RESPONDERS
Implantation rates
Time frame: 2 weeks
FOLLICULAR FLUSHING ON CYCLE OUTCOMES IN POOR RESPONDERS
Pregnancy rates
Time frame: 2 weeks
FOLLICULAR FLUSHING ON CYCLE OUTCOMES IN POOR RESPONDERS
Live birth rates
Time frame: 2 weeks
FOLLICULAR FLUSHING ON CYCLE OUTCOMES IN POOR RESPONDERS
Operating room anesthesia time.
Time frame: 2 weeks
FOLLICULAR FLUSHING ON CYCLE OUTCOMES IN POOR RESPONDERS
Luteal E2 level.
Time frame: 2 weeks
FOLLICULAR FLUSHING ON CYCLE OUTCOMES IN POOR RESPONDERS
Luteal P4 level.
Time frame: 2 weeks
This study is completed, as verified in Jun 2015. You cannot join it, but the record below documents what was studied.
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Weill Medical College of Cornell University