Changes in Intestinal Permeability and Systemic Inflammation Following Fast Food Overfeeding.

• Normal fasting plasma glucose and glycated hemoglobin (HbA1c) \<42 mmol/mol
• Body mass index (BMI) between 18.5 and 25 kg/m2
• Hemoglobin >8.3 mmol/l
• Participants must consume a ordinary mixed diet and not follow restrictive or specialized dietary regimens
• Age between 20 and 40 years
• Oral and written informed consent

Exclusion Criteria:

• Diabetes
• Known gastrointestinal disease, major or recent abdominal surgery
• First degree relatives with inflammatory bowel disease
• History of BMI > 30.
• Current or history of psychiatric eating disorder
• Regular use of nicotine products in any form
• Regular alcohol intake above 18 units per week
• Fasting plasma triacylglycerol indicating dyslipidemia (≥2 mmol/l)

Nephropathy (estimated glomerular filtration rate (eGFR) \<60 ml/min and/or albuminuria with an albumin to creatinine ratio of >30 μg/mg)

• Known liver disease and/or alanine aminotransferase (ALT) >2 × upper reference limits
• Signs of liver fibrosis and/or steatosis evaluated by FibroScan (CAP value >238 dB/m and/or kPa >5.0) and/or FIB-4 score (>1.45)
• Regular use of prescription medication or treatment with systemic antibiotics less than three weeks before the screening visit.
• Use of over-the-counter medication or any dietary supplements known to alter gastrointestinal function, including probiotics, for the period of the study and three weeks prior to screening
• More than two weekly strength and/or aerobic training sessions on a regular basis
• Physical or mental conditions incompatible with magnetic resonance scanning modalities, including implanted metal objects.
• Not willing to agree to use effective contraception during and for 90 days after end of the diet intervention. Acceptable contraception includes condom use and/or ensuring that the female partner(s) uses highly effective contraception.
• Any condition that the investigator feels would interfere with study completion