Extension Study of the PUMCH-E101 Clinical Trial：Safety and Efficacy Study of PUMCH-E101 Injection in the Contralateral Eye

1. Patients who have undergone treatment for one eye within the PUMCH-E101 protocol and have completed a one-year follow-up period.
2. The participant is willing to participate voluntarily, sign an informed consent form, and is willing and able to adhere to the visit schedule, follow the treatment plan, undergo laboratory tests, and comply with other study procedures.
3. Male or female participants between the ages of 8 and 45.
4. The study eye's best corrected visual acuity (BCVA) measured using the ETDRS (Early Treatment Diabetic Retinopathy Study) visual acuity chart is not higher than 78 letters (including the boundary value), which is equivalent to no better than 20/32 on the Snellen visual acuity chart.
5. During the screening process, the blood pregnancy test results are negative for women of childbearing age (e.g., those who have not undergone surgical sterilization or are postmenopausal for less than 1 year). Both male and female participants of childbearing age and their partners agree to use effective contraceptive measures throughout the study period and for at least 12 months following the administration of the medication.

Exclusion Criteria:

1. Opacity of refractive media or inability to dilate pupils in the study eye that significantly interferes with visual acuity detection, anterior segment or fundus assessment;
2. Presence of diabetic retinopathy, retinal vein occlusion, pathological myopia, retinal detachment, or other conditions in the study eye that are assessed by the investigator as affecting the safety of the subject or the validity of the study;
3. Any intraocular surgery in the study eye within 3 months prior to screening;
4. Active intraocular or periocular infection (such as blepharitis, conjunctivitis, keratitis, scleritis, etc.) in the study eye;
5. History of uveitis in either eye；
6. Those with diffuse intravascular coagulation and obvious bleeding tendency (such as hemoptysis, hematemesis, severe purpura, etc.) within 3 months before screening;
7. History of myocardial infarction, unstable angina, coronary revascularization, cerebrovascular accident (including TIA), history of other thromboembolic diseases (such as thromboembolic angiitis, pulmonary embolism, deep vein thrombosis, portal vein thrombosis, etc.), New York Heart Association (NYHA) grade ≥ II cardiac insufficiency, severe unstable ventricular arrhythmia, within 6 months prior to screening;
8. Subjects with systemic immune diseases (including systemic lupus erythematosus, ankylosing spondylitis, rheumatoid arthritis, etc.);
9. Diabetic patients with any of the following conditions: Known macrovascular complications or Glycosylated hemoglobin at screening（HbA1c）>7.5% or Those who have received more than two oral hypoglycemic drugs or received insulin or GLP-1 receptor agonists therapies;
10. Hypertensive patients with poor blood pressure control (defined as: systolic blood pressure ≥ 160 mmHg or diastolic blood pressure ≥100 mmHg when the subject is seated after receiving antihypertensive medication);
11. Any uncontrollable clinical illness (such as severe psychiatric, respiratory and other systemic diseases and history of malignant tumors);
12. Subjects with abnormal liver and kidney function: alanine aminotransferase (ALT)/aspartate aminotransferase (AST) ≥ 2 times the upper limit of normal; Total bilirubin ≥ 1.5 times the upper limit of normal, creatinine and urea/urea nitrogen ≥ 1.5 times the upper limit of normal;
13. Subjects with abnormal coagulation function: prothrombin time (PT) > upper limit of normal value of 3 seconds or activated partial thromboplasting time (APTT) > upper limit of normal value of 10 seconds; Haemoglobin (HGb) \< 10 g/dL;
14. Those who are positive for hepatitis B surface antigen (HBsAg), hepatitis C virus (HCV) antibody, treponema pallidum antibody and human immunodeficiency virus (HIV) antibody;
15. Those who are known to be allergic to the therapeutic drugs or diagnostic drugs used in the study protocol, including the investigational products, etc.;
16. Those who have used anticoagulant or antiplatelet drugs within 7 days before dosing;
17. Currently using or may need to use drugs that can cause crystalline toxicity or retinal toxicity (such as deferoxamine, chloroquine/hydroxychloroquine, tamoxifen, ethambutol, etc.);
18. Those who have a history of surgical operation within 1 month before screening, and/or currently have unhealed wounds (wound degree> stage III), moderate to severe ulcers, and fractures;
19. Subjects with systemic infectious diseases requiring systemic treatment (oral, intramuscular or intravenous) at the time of screening;
20. Those who have received any AAV gene therapy products in the past;
21. Pregnant or lactating females;
22. Those who have participated in any clinical trial of drugs (excluding vitamins and minerals) within 3 months before screening;
23. Other individuals who need to be excluded, as determined by the investigator.