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RecruitingNCT07768228Updated Aug 17, 2026

The Effect of L-Carnitine on Wound Healing in Patients With Diabetic Foot Ulcer.

A Phase 2 interventional study of L- carnitine in Diabetic Foot Ulcer and Diabetic Foot Ulcer (DFU), sponsored by Ain Shams University. Recruiting at 2 sites in Egypt. Open to participants aged 30 Years to 75 Years. Per ClinicalTrials.gov, last updated 2026-08-17.

Sponsored by Ain Shams University · Phase 2, Interventional, and Treatment

Phase
Phase 2
Study type
Interventional
Enrollment
50
Allocation
Randomized
Ages
30 Years to 75 Years
Sex
All
01

Study summary

Primary outcome: To evaluate the efficacy of L-Carnitine supplementation on length and depth ulcer size

Secondary outcome: To investigate the effect of L-Carnitine supplements on:

  1. Vascular endothelial growth factor (VEGF)
  2. Inflammatory markers TNF-a (Tumor necrosis factor alpha).
  3. Hemoglobin A1C (HbA1c) And Lipid profile (cholesterol, triglycerides, high-density Lipoprotein (HDL) and low-density lipoprotein (LDL)).
  4. To assess the type and likelihood of any adverse drug reaction that can be related to L-Carnitine supplementation.
  5. QOL (DIABETES 39)
Read the detailed description

Diabetic foot ulcer (DFU) is a major complication of diabetes that may develope as a result of multiple factors, including diabetic neuropathy, ischemia, infection and local trauma. DFU is a challenging complication that requires a multidisciplinary approaches to control blood glucose level along with standard Care of wound which will decrease the risk of amputation in participants with diabetic foot ulcers.

The investigators hypothesize that L-Carnitine supplementation may have potential benefits for participants with diabetic foot ulcers; mainly due to its antioxidant activity, its wound healing effect on damage skin, and anti-inflammatory properties.

The aim of the study is to evaluate the efficacy and safety of L-Carnitine supplementation in participants with diabetic foot ulcer. Secondary outcome is to investigate the effect of L-Carnitine supplements on; VEGF, TNF-a, Lipid profile, HbA1c, and quality of life.

This would be a randomized, open label-controlled trial where a total of 50 participants with DFU will be enrolled in the study. These participants will be randomly assigned in a 1:1 ratio in two groups: Group 1(treatment group) will include 25 participants will receive 1000mg of L-Carnitine twice daily for 12 Weeks in addition to standard therapy. Group 2(control group) will include 25 participants who will receive standard therapy. Standard treatment will include Anti -diabetic treatment (insulin or oral antidiabetic drugs), in addition to standard wound care treatment (saline and silver dressing).

A 5ml blood sample would be collected by a trained nurse at baseline and after 12 weeks to assess its effect on VEGF, TNF-a, Lipid profile, and HbA1c.

02

Conditions studied

  • Diabetic Foot Ulcer
  • Diabetic Foot Ulcer (DFU)

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Keywords

  • L-Carnitine
  • Diabetic Foot Ulcer
  • DFU
03

Who can participate

Ages eligible
30 Years to 75 Years
Sexes eligible
All
Accepts healthy volunteers
No

Inclusion criteria

  • Adult Male or Female patients with diabetic neuropathy mean age (30-75) years old.
  • Having Diabetic neuropathy and Diabetic foot ulcer grade 1-2 (mild- moderate) according to Wagner classification.
  • Uncontrolled diabetic patients (HbA1c). \< 9 duration of diabetic (5 - 10) years.
  • All patient treated with stable dose of anti-diabetic medication priors and during the period of the study.

Exclusion criteria

Exclusion Criteria:

  • Patients with other causes of ulcers.
  • Patient taking any antioxidant supplements including vitamin E, ascorbic acid, omega 3 fatty acid, Coenzyme Q10 within three months prior to enrollment in the study.
  • Patient with autoimmune disease.
  • Patient with liver disease or kidney disease.
  • Patient taking medications for cancer.
  • Patient with thyroid disease.
04

Study design

Phase
Phase 2
Primary purpose
Treatment
Allocation
Randomized
Intervention model
Parallel assignment
Masking
None (open label)
Enrollment
50 participants (estimated)

Study arms

  • Experimental
    Treatment

    To receive oral L-carnitine plus standard care therapy

    Drug: L- carnitine

  • No intervention
    Control

    To receive only standard of care therapy

Interventions

  • DrugL- carnitine

    oral L-carnitine

05

What researchers measure

Primary outcomes

  1. Change in Diabetic Foot Ulcer area

    Diabetic Foot Ulcer area will be measured at baseline and after 12 weeks using the ulcer length and width measurements. The change in ulcer area from baseline to 12 weeks will be assessed between the treatment (L-carnitine) and control groups. Unit of measure: cm2

    Time frame: At baseline and the end of the study (after 12 weeks)

  2. Change in Diabetic Foot Ulcer depth

    Diabetic Foot Ulcer depth will be measured at baseline and after 12 weeks. The change in ulcer depth from baseline to 12 weeks will be assessed between the treatment (L-carnitine) and control groups. unit of measure: cm

    Time frame: At baseline and the end of the study (after 12 weeks)

Secondary outcomes

  1. Change in Vascular endothelial growth factor (VEGF) level

    Serum Vascular endothelial growth factor (VEGF) level measured at baseline and after 12 weeks of treatment. The change from baseline to 12 weeks will be assessed.

    Time frame: At Baseline and the end of the study (after 12 weeks)

  2. Change in Tumor Necrosis Factor-alpha (TNF-a) level

    Serum TNF-a level measured at baseline and after 12 weeks of treatment. The change from baseline to 12 weeks will be assessed.

    Time frame: At Baseline and the end of the study (after 12 weeks)

  3. Change in Hemoglobin A1c (HbA1c) level

    HbA1c level measured at baseline and after 12 weeks of treatment. The change from baseline to 12 weeks will be assessed.

    Time frame: At baseline and the end of the study (after 12 weeks)

  4. Change in Lipid profile level

    The lipid parameters will be measured at baseline and after 12 weeks of treatment. The change from baseline to 12 weeks will be assessed.

    Time frame: At baseline and the end of the study (after 12 weeks)

  5. Change in Diabetes-39 Quality of Life Score.

    Quality of life will be assessed using the Diabetes-39 (D-39) questionnaire at baseline and after 12 weeks of treatment. The change in the total D-39 score from baseline to 12 weeks will be assessed.

    Time frame: At Baseline and the end of the study (after 12 weeks)

  6. Number of participants with adverse events.

    The number of participants experiencing adverse events during the study period will be assessed, including the type and occurrence of adverse events potentially related to L-Carnitine supplementation.

    Time frame: At baseline and the end of the study (after 12 weeks)

06

Study locations

1 of 2 sites recruiting
  • Faculty of Pharmacy, Ain Shams University
    Cairo, Cairo Governorate 11566, Egypt
    Recruiting
  • Faculty of Pharmacy, Ain Shams University
    Cairo, Cairo Governorate 11566, Egypt
    Active, not recruiting
07

References and documents

Publications

  • Aldendail CF, Chen P, Dibble HS, Baute Penry V. A Comprehensive Review of Safety, Efficacy, and Indications for the Use of Alpha-Lipoic Acid and Acetyl-L-Carnitine in Neuropathic Pain. Integr Med (Encinitas). 2024 Jul;23(3):32-39. PubMed 39114278 ↗
  • Scioli MG, Lo Giudice P, Bielli A, Tarallo V, De Rosa A, De Falco S, Orlandi A. Propionyl-L-Carnitine Enhances Wound Healing and Counteracts Microvascular Endothelial Cell Dysfunction. PLoS One. 2015 Oct 16;10(10):e0140697. doi: 10.1371/journal.pone.0140697. eCollection 2015. PubMed 26473356 ↗
  • Fathizadeh H, Milajerdi A, Reiner Z, Kolahdooz F, Asemi Z. The effects of L-carnitine supplementation on glycemic control: a systematic review and meta-analysis of randomized controlled trials. EXCLI J. 2019 Aug 19;18:631-643. doi: 10.17179/excli2019-1447. eCollection 2019. PubMed 31611746 ↗
  • Yahyapoor F, Sedaghat A, Feizi A, Bagherniya M, Pahlavani N, Khadem-Rezaiyan M, Safarian M, Islam MS, Zarifi SH, Arabi SM, Norouzy A. The effects of l-Carnitine supplementation on inflammatory markers, clinical status, and 28 days mortality in critically ill patients: A double-blind, randomized, placebo-controlled trial. Clin Nutr ESPEN. 2022 Jun;49:61-67. doi: 10.1016/j.clnesp.2022.04.001. Epub 2022 Apr 9. PubMed 35623869 ↗
  • Galiano RD, Tepper OM, Pelo CR, Bhatt KA, Callaghan M, Bastidas N, Bunting S, Steinmetz HG, Gurtner GC. Topical vascular endothelial growth factor accelerates diabetic wound healing through increased angiogenesis and by mobilizing and recruiting bone marrow-derived cells. Am J Pathol. 2004 Jun;164(6):1935-47. doi: 10.1016/S0002-9440(10)63754-6. PubMed 15161630 ↗
  • Nasole E, Nicoletti C, Yang ZJ, Girelli A, Rubini A, Giuffreda F, Di Tano A, Camporesi E, Bosco G. Effects of alpha lipoic acid and its R+ enantiomer supplemented to hyperbaric oxygen therapy on interleukin-6, TNF-alpha and EGF production in chronic leg wound healing. J Enzyme Inhib Med Chem. 2014 Apr;29(2):297-302. doi: 10.3109/14756366.2012.759951. Epub 2013 Jan 30. PubMed 23360079 ↗
  • Dworzanski J, Strycharz-Dudziak M, Kliszczewska E, Kielczykowska M, Dworzanska A, Drop B, Polz-Dacewicz M. Glutathione peroxidase (GPx) and superoxide dismutase (SOD) activity in patients with diabetes mellitus type 2 infected with Epstein-Barr virus. PLoS One. 2020 Mar 25;15(3):e0230374. doi: 10.1371/journal.pone.0230374. eCollection 2020. PubMed 32210468 ↗
  • Deng L, Du C, Song P, Chen T, Rui S, Armstrong DG, Deng W. The Role of Oxidative Stress and Antioxidants in Diabetic Wound Healing. Oxid Med Cell Longev. 2021 Feb 4;2021:8852759. doi: 10.1155/2021/8852759. eCollection 2021. PubMed 33628388 ↗
  • Armstrong DG, Nguyen HC, Lavery LA, van Schie CH, Boulton AJ, Harkless LB. Off-loading the diabetic foot wound: a randomized clinical trial. Diabetes Care. 2001 Jun;24(6):1019-22. doi: 10.2337/diacare.24.6.1019. PubMed 11375363 ↗
  • Armstrong DG, Lavery LA, Nixon BP, Boulton AJ. It's not what you put on, but what you take off: techniques for debriding and off-loading the diabetic foot wound. Clin Infect Dis. 2004 Aug 1;39 Suppl 2:S92-9. doi: 10.1086/383269. PubMed 15306986 ↗
  • Golbidi S, Badran M, Laher I. Diabetes and alpha lipoic Acid. Front Pharmacol. 2011 Nov 17;2:69. doi: 10.3389/fphar.2011.00069. eCollection 2011. PubMed 22125537 ↗

Individual participant data

Plan to share: No

08

Registry details

Key details

Study ID
NCT07768228
Lead sponsor
Ain Shams University
Responsible party
Sponsor
First posted
Aug 17, 2026
Start date
Sep 1, 2026 (estimated)
Primary completion
Jul 1, 2027 (estimated)
Completion
Aug 1, 2027 (estimated)
Last update
Aug 17, 2026

Study contacts

Mariam Hosam, Bachelor degree in Pharmacy
Contact
Mariam.hossam@pharma.asu.edu.eg
2+ 01115772704

Oversight

Data monitoring committee
No
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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