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Not yet recruitingNCT07652866Updated Jun 18, 2026

Mechanisms of Sulforaphane Supplementation in Alleviating Negative Symptoms and Cognitive Impairment in Schizophrenia

An interventional study of Placebo and Sulforaphane in Schizophrenia Disorder, sponsored by Second Xiangya Hospital of Central South University. Not yet recruiting at 1 site in China. Open to participants aged 12 Years to 45 Years. Per ClinicalTrials.gov, last updated 2026-06-18.

Sponsored by Second Xiangya Hospital of Central South University · Not applicable, Interventional, and Treatment

Phase
Not applicable
Study type
Interventional
Enrollment
60
Allocation
Randomized
Ages
12 Years to 45 Years
Sex
All
01

Study summary

The goal of this randomized, double-blind, placebo-controlled clinical trial with an open-label extension is to evaluate whether sulforaphane can improve negative symptoms and cognitive impairment, and to explore its underlying mechanisms in patients with schizophrenia (aged 12-45 years, both sexes, stable on antipsychotic medication). The study duration includes 12 weeks of double-blind treatment followed by a 12-week open-label extension. In the randomized controlled double-blind phase, a total of 60 participants will be randomized 1:1 to receive either six oral tablets (411 μmol GR) of sulforaphane (SFN group, n = 30) or placebo (placebo group, n = 30) for 12 weeks. In the open-label phase, participants will choose whether to continue taking the drugs originally assigned. The primary outcome is the change in PANSS and BNSS scores during the randomized double-blind phase. Secondary outcomes include changes in brain MRI measures, as well as changes in MCCB, CGI-SI, CGI-GI, PSP, SNS, and SAFTEE scores during the randomized double-blind phase; and changes in PANSS, BNSS, and MCCB scores during the open-label phase.SAFTEE scale, serious adverse event record and blood test will be used for safety monitoring.

02

Conditions studied

  • Schizophrenia Disorder

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03

Who can participate

Ages eligible
12 Years to 45 Years
Sexes eligible
All
Accepts healthy volunteers
No

Inclusion criteria

  1. Diagnosis of schizophrenia according to DSM-5 criteria.
  2. First-episode or illness duration ≤ 10 years, but currently in a non-acute phase of schizophrenia.
  3. Negative symptoms present for ≥ 6 months prior to study entry. Patients must be outpatients or hospitalized for social reasons rather than symptom exacerbation.
  4. PANSS negative subscale (7 items) total score ≥ 20; at least one negative item score > 3; no change > 3 points between screening and baseline. PANSS positive subscale items related to agitation (P4 excitement, P6 suspiciousness/persecution, P7 hostility, G8 uncooperativeness, G14 poor impulse control) each ≤ 4.
  5. Currently taking ≤ 2 antipsychotic medications.
  6. Antipsychotic regimen remains unchanged during the study period.
  7. No anticipated relocation, transportation difficulties, or access problems that would interfere with study participation.
  8. Able to understand and comply with study procedures, complete all required tests and examinations, communicate well with the investigator, and voluntarily provide written informed consent

Exclusion criteria

Exclusion Criteria:

  1. Psychiatric symptoms attributable to any other DSM-5 diagnosis besides schizophrenia.
  2. History of substance dependence, or psychotic symptoms caused by other medical conditions.
  3. Calgary Depression Scale for Schizophrenia (CDSS) total score > 6.
  4. Barnes Akathisia Rating Scale (BARS) score indicating at least moderate akathisia.
  5. Current or past major physical illness, neurological disorder, or traumatic brain injury affecting brain structure/function.
  6. Suicidal attempt or current suicidal ideation.
  7. Currently receiving antidepressants, mood stabilizers; or use of rTMS, MECT, or systematic psychotherapy within 3 months or for the current episode.
  8. Current use of medications that may affect cognitive function, such as Ginkgo biloba extract, minocycline, selegiline.
  9. Presence of hepatic or renal insufficiency, severe gastrointestinal, respiratory, endocrine, or hematologic disorders, or disorders of absorption or metabolism.
  10. Pregnant or breastfeeding women.
04

Study design

Phase
Not applicable
Primary purpose
Treatment
Allocation
Randomized
Intervention model
Parallel assignment
Masking
Double (Participant, Outcomes assessor)
Enrollment
60 participants (estimated)

Study arms

  • Placebo comparator
    Placebo

    Dietary Supplement: Placebo

  • Experimental
    sulforaphane

    Dietary Supplement: Sulforaphane

Interventions

  • Dietary supplementPlacebo

    Participants take 6 tablets of matching placebo daily for the first 3 months (randomized double-blind phase). During the subsequent 3-month open-label extension phase, those who choose to continue their original assigned medication also take 6 tablets of matching placebo per day, i.e., six placebo tablets daily. Both active and placebo tablets are manufactured uniformly by Shenzhen Fushan Biotech Co., Ltd. (China), with identical appearance and similar smell and taste.

  • Dietary supplementSulforaphane

    Participants take 6 tablets of sulforaphane daily for the first 3 months (randomized double-blind phase). During the subsequent 3-month open-label extension phase, those who choose to continue their original assigned medication also take 6 tablets of sulforaphane per day, equivalent to a dosage of six active tablets (411 μmol GR). The sulforaphane-producing dietary supplement, ZHIYINGUOSU, is provided at no cost by Shenzhen Fushan Biotech Co., Ltd. (China).

05

What researchers measure

Primary outcomes

  1. Change from baseline in the Positive and Negative Syndrome Scale (PANSS) negative subscale score

    PANSS negative subscale assesses severity of negative symptoms (score range 7-49, higher = worse). Change scores are calculated as the score at each time point (6 and 12 weeks) minus the baseline score. A negative change at either time point indicates improvement.

    Time frame: Baseline to 6 and 12 weeks

  2. Change from baseline in Brief Negative Symptom Scale (BNSS) score

    The BNSS measures negative symptom severity (total score 0-78, higher = worse). Change from baseline = score at week minus baseline score (calculated separately for week 6 and week 12). A negative change at either time point indicates improvement.

    Time frame: Baseline to 6 and 12 weeks

Secondary outcomes

  1. Change from baseline in MATRICS Consensus Cognitive Battery (MCCB) score

    The MCCB assesses seven cognitive domains. Positive change indicates cognitive improvement.

    Time frame: Baseline to 12 weeks

  2. Brain imaging changes

    Evaluation of brain imaging changes from baseline at week 12. The scanning protocol includes structural imaging, functional imaging, diffusion imaging, myelin imaging, and magnetic resonance spectroscopy imaging, to systematically evaluate brain structure, functional connectivity, white matter microstructure, myelin integrity, and neurometabolic profiles in patients with schizophrenia.

    Time frame: Baseline to 12 weeks

  3. Change in serum biomarker levels

    Evaluation of peripheral blood biomarkers of inflammation, oxidative stress and metabolism

    Time frame: Baseline to 12 weeks

  4. Change in PANSS negative subscale score (open-label extension)

    Evaluation of the change from week 12 in the negative symptom subscale of PANSS at week 24. Change = score at 24 weeks minus score at week 12. Negative change indicates further improvement during extension.

    Time frame: Week 12 to 24

  5. Change in Brief Negative Symptom Scale (BNSS) (open-label extension)

    BNSS total score (0-78, higher=worse). Change = 24-week score minus week-12 score. Negative change indicates further improvement during extension.

    Time frame: Week 12 to 24

  6. Change in the composite score of MATRICS Consensus Cognitive Battery (MCCB) (open-label extension)

    Evaluation of the score and change from week 12 in MCCB composite score at week 24. Change = 24-week score minus week-12 score. Positive change indicates further improvement during extension

    Time frame: Week 12 to 24

  7. Change in serum biomarker levels (open-label extension)

    Serum biomarker concentrations. Change = level at 24 weeks minus level at week 12.

    Time frame: Week 12 to 24

  8. Clinical Global Impression - Severity of Illness (CGI-SI) score

    CGI-SI rates illness severity on a 7-point scale (1=normal, 7=extremely ill). Change = score at week minus baseline score (separately for week 6 and week 12). Negative change indicates improvement.

    Time frame: Baseline to 6 and 12 weeks

  9. Clinical Global Impression - Global Improvement (CGI-GI) score

    The CGI-GI rates overall change on a 7-point scale (1=very much improved, 4=no change, 7=very much worse). The score at each time point (6 and 12 weeks) directly reflects improvement since baseline; lower scores mean greater improvement.

    Time frame: Week 6 and week 12

  10. Change in Self-rating Negative Symptom Scale (SNS) score

    The SNS is a self-reported scale completed by the patient to assess the severity of negative symptoms. Each item is scored on a 3-point scale (0=strongly disagree, 1=slightly agree, 2=completely agree), yielding a total score ranging from 0 to 40, with higher scores indicating more severe negative symptoms. Change scores are calculated as the score at each time point (6 and 12 weeks) minus the baseline score. A negative change at either time point indicates symptomatic improvement.

    Time frame: Baseline to 6 and 12 weeks

  11. Change in Personal and Social Performance (PSP) total score

    The PSP total score ranges 1-100 (higher = better personal and social functioning). Change = week score minus baseline score (separately for week 6 and week 12). Positive change means functional improvement.

    Time frame: Baseline to 6 and 12 weeks

  12. Change in Barnes Akathisia Rating Scale (BARS) total score

    The BARS measures akathisia severity (total score 0-14, higher = worse). Change = week score minus baseline score (separately for week 6 and week 12). Negative change indicates reduced akathisia.

    Time frame: Baseline to 6 and 12 weeks

  13. Change in Systematic Assessment for Treatment Emergent Events (SAFTEE) total score

    The SAFTEE total score reflects adverse event burden (higher score = greater burden). Change = week score minus baseline score (separately for week 6 and week 12). Negative change means reduction in adverse events.

    Time frame: Baseline to 6 and 12 weeks

  14. Clinical Global Impression - Severity of Illness (CGI-SI) score (open-label extension)

    CGI-SI rates illness severity on a 7-point scale (1=normal, 7=extremely ill).

    Time frame: Week 24

  15. Clinical Global Impression - Global Improvement (CGI-GI) score (open-label extension)

    The CGI-GI rates overall change on a 7-point scale (1=very much improved, 4=no change, 7=very much worse). The score at 24 weeks directly reflects improvement since baseline; lower scores mean greater improvement.

    Time frame: Week 24

  16. Change in Self-rating Negative Symptom Scale (SNS) score (open-label extension)

    The SNS is a patient-reported outcome measuring negative symptom severity using a 3-point scale per item (0=strongly disagree, 1=slightly agree, 2=completely agree), with a total score range of 0 to 40 (higher = worse). Change is calculated as the score at 24 weeks minus the score at week 12. A negative change indicates further improvement in negative symptoms during the extension phase.

    Time frame: Week 12 to 24

  17. Change in Personal and Social Performance (PSP) total score (open-label extension)

    The PSP total score ranges 1-100 (higher = better personal and social functioning). Change = week 24 score minus week 12 score. Positive change means functional improvement.

    Time frame: Week 12 to 24

  18. Change in BARS total score (open-label extension)

    The BARS measures akathisia severity (total score 0-14, higher = worse). Change = week score minus baseline score (separately for week 6 and week 12). Negative change indicates reduced akathisia.

    Time frame: Week 12 to 24

  19. Change in Systematic Assessment for Treatment Emergent Events (SAFTEE) total score (open-label extension)

    he SAFTEE total score reflects adverse event burden (higher score = greater burden). Change = week 24 score minus week 12 score. Negative change means reduction in adverse events.

    Time frame: Week 12 to 24

Other outcomes

  1. Complete Blood Count (CBC)

    Evaluation of red blood cell count, white blood cell count, platelet count, hemoglobin, neutrophil percentage, lymphocyte percentage, and monocyte percentage, as well as change from baseline at week 12 and week 24.

    Time frame: Baseline, week 12 and week 24

  2. Blood Biochemistry

    Evaluation of liver function (ALT, AST, ALP, total bilirubin, direct bilirubin, total protein, albumin), as well as change from baseline at week 12 and week 24.

    Time frame: Baseline, week 12 and week 24

  3. Biochemistry of renal function (creatinine, urea)

    Evaluation of renal function (creatinine, urea), as well as change from baseline at week 12 and week 24.

    Time frame: Baseline, week 12 and week 24

06

Study locations

1 site
07

References and documents

Individual participant data

Plan to share: Undecided

No publications or documents are linked to this record.

08

Registry details

Key details

Study ID
NCT07652866
Lead sponsor
Second Xiangya Hospital of Central South University
Responsible party
Jing Huang (Professor, Second Xiangya Hospital of Central South University) — Principal investigator
First posted
Jun 17, 2026
Start date
Jun 2, 2026 (estimated)
Primary completion
Dec 31, 2028 (estimated)
Completion
Dec 31, 2028 (estimated)
Last update
Jun 18, 2026

Study contacts

Jing Huang, Prof.
Contact
jinghuangserena@csu.edu.cn
+8613107212438

Oversight

Data monitoring committee
Yes
FDA-regulated drug
No
FDA-regulated device
No
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