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Not yet recruitingNCT07585136Updated Aug 31, 2026

Stem Cell Mobilization and Apheresis for Life-threatening Blood Disorders

A Phase 1 interventional study of Filgrastim and Plerixafor in Bone Marrow Failure Syndrome, sponsored by St. Jude Children's Research Hospital. Not yet recruiting at 1 site in United States. Open to participants aged 18 Years to 25 Years. Per ClinicalTrials.gov, last updated 2026-08-31.

Sponsored by St. Jude Children's Research Hospital · Phase 1, Interventional, and Other

Phase
Phase 1
Study type
Interventional
Enrollment
12
Allocation
Not applicable
Ages
18 Years to 25 Years
Sex
All
01

Study summary

The purpose of this study is to investigate mobilization and collection of HSPCs in patients with bone marrow failure syndromes (BMFS) using granulocyte-colony stimulating factor (otherwise known as Filgrastim) with plerixafor to demonstrate safety and feasibility of collecting HSPCs to advance gene therapy.

Primary objective:

- To characterize the safety of Filgrastim plus plerixafor in participants with bone marrow failure syndromes as determined by the incidence of adverse events (AEs).

Secondary Objectives:

  • To characterize the feasibility of HSPC mobilization using Filgrastim plus plerixafor as determined by peripheral blood CD34+ counts.
  • To measure the mobilization effects of Filgrastim plus plerixafor in the peripheral blood in participants as determined by peak peripheral blood CD34+ counts.
  • To estimate efficacy of Filgrastim plus plerixafor for HSPC mobilization and apheresis collection in participants as determined by the yield of CD34+ cells (CD34+ cells/kg).
Read the detailed description

This is a phase I, open-label, single-center study to evaluate the safety of Filgrastim plus plerixafor stem cell mobilization and apheresis in patients with BMFS. This study will include a screening period with labs, physical examination, and bone marrow evaluation at least 6 months prior to mobilization and apheresis, an intervention period that includes mobilization and apheresis of patient HSPCs, and outpatient follow-up within 7-10 days after intervention. Study staff will follow up with the participant via telephone approximately 30 days after mobilization and apheresis. A bone marrow evaluation will be done within 6 months post-intervention.

02

Conditions studied

  • Bone Marrow Failure Syndrome
03

Who can participate

Ages eligible
18 Years to 25 Years
Sexes eligible
All
Accepts healthy volunteers
No

Inclusion criteria

  • Participants with a bone marrow failure syndrome with an identified genetic cause willing to donate autologous HSPCs for advancing gene therapy
  • Age ≥ 18 years - 25 years
  • The following hematological parameters need to be met (regardless of transfusion or growth factor support)

    • Hb > 8 g/dL
    • ANC > 500/mm3
    • Platelet > 30,000/mm3
  • Bone marrow evaluation within the preceding 6 months prior to mobilization and apheresis
  • Participants should either have a central venous catheter (CVC) in place, be able to undergo apheresis without requiring a CVC, or agree to having a temporary apheresis catheter placed
  • Karnofsky score >80
  • Negative serologic tests for syphilis, hepatitis B and C, HIV, and HTLV-1/II
  • Female participants of childbearing age should have a negative serum pregnancy test within one week of beginning Filgrastim and plerixafor administration

Exclusion criteria

Exclusion Criteria:

  • Participant with sickle cell disease
  • Participant who has had a prior autologous or allogeneic HSCT
  • Active viral, bacterial, fungal, or parasitic infection
  • Total bilirubin >2.5x ULN or transaminases >5x ULN
  • Moderate or severe renal failure defined as serum/plasma creatinine >1.5 mg/dL and an estimated glomerular filtration rate (eGFR) \< 60 mL/min/1.73 m2 based on the CKD-Epi equation or the St. Jude equation
  • Diagnosis of MDS or other hematologic malignancy
  • History of malignancy
  • Known allergy to or contraindication for Filgrastim or plerixafor administration, or medications routinely administered during apheresis
  • Splenomegaly (size greater than upper limit of normal on examination)
  • Any disease or concomitant process that is not compatible with the study as per investigator opinion
  • Concomitant treatment with alternative investigational agent or participation in another clinical trial with an investigational drug within 5 half-lives of the investigational agent
  • Unwillingness to use a highly effective method of contraception for 1 month after plerixafor or GCSF
  • Pregnancy
  • Inability or unwillingness of research participant to give written informed consent.
04

Study design

Phase
Phase 1
Primary purpose
Other
Allocation
Not applicable
Intervention model
Single group
Masking
None (open label)
Enrollment
12 participants (estimated)

Study arms

  • Experimental
    BDSTEM Treatment

    Participants in this study will receive a twice daily dose of Filgrastim (GCSF) (5 mcg/kg BID) SQ starting on day 1 for 5 days followed by a single dose of SQ plerixafor (0.24 mg/kg) on day 5 followed by collection of CD34+ HSPCs via apheresis. A portion of cells collected from the participant will be stored as backup to be used toward future gene therapy endeavors. The remaining cells will be donated for research studies

    Drug: Filgrastim · Drug: Plerixafor · Procedure: Leukapheresis

Interventions

  • DrugFilgrastim

    Administered twice daily dose starting on day 1 for 5 days.

  • DrugPlerixafor

    Administered on day 5 via IV.

  • ProcedureLeukapheresis

    Peripheral venous access or through a central venous catheter approximately 4-5 hours after the dose of plerixafor is given.

05

What researchers measure

Primary outcomes

  1. Incidence of treatment-emergent adverse events following filgrastim plus plerixafor administration

    Safety will be assessed by the incidence, type, and severity of adverse events occurring after administration of filgrastim plus plerixafor in participants with bone marrow failure syndromes.

    Time frame: From initiation of drug administration through Day +7 to +10 follow-up

Secondary outcomes

  1. Number of participants achieving peripheral blood CD34+ counts ≥5 cells/µL

    Feasibility of hematopoietic stem and progenitor cell mobilization will be assessed by peripheral blood CD34+ cell counts measured after plerixafor administration and prior to or during apheresis.

    Time frame: From initiation of plerixafor administration through completion of apheresis, or 6 hours after drug administration if apheresis is not performed

  2. Peripheral blood CD34+ kinetics following filgrastim plus plerixafor administration

    Peripheral blood CD34+ cell counts will be measured after plerixafor administration and prior to or during apheresis.

    Time frame: After plerixafor administration through completion of apheresis, or 6 hours after drug administration if apheresis is not performed

  3. Observed CD34+ cell yield after 1 blood volume apheresis

    CD34+ cell yield (CD34+ cells/kg) collected after processing 1 blood volume during apheresis following filgrastim plus plerixafor administration.

    Time frame: At completion of 1 blood volume apheresis on Day 5

  4. Estimated total CD34+ cell yield from projected full-volume apheresis

    Estimated total CD34+ cell yield (CD34+ cells/kg) projected from the observed yield after processing 1 blood volume during apheresis.

    Time frame: At completion of 1 blood volume apheresis on Day 5

06

Study locations

1 site
  • Saint Jude Children's Research Hospital
    Memphis, Tennessee 38105-2794, United States
07

References and documents

Individual participant data

Plan to share: Yes — Individual participant de-identified datasets containing the variables analyzed in the published article will be made available (related to the study primary or secondary objectives contained in the publication). Supporting documents such as the protocol, statistical analyses plan, and informed consent are available through the CTG website for the specific study. Data used to generate the published article will be made available at the time of article publication. Investigators who seek access to individual level de-identified data will contact the computing team in the Department of Biostatistics (ClinTrialDataRequest@stjude.org) who will respond to the data request.

Supporting information: Study protocol, Sap, Icf

08

Registry details

Key details

Study ID
NCT07585136
Lead sponsor
St. Jude Children's Research Hospital
Responsible party
Sponsor
First posted
May 13, 2026
Start date
Nov 2026 (estimated)
Primary completion
Dec 1, 2028 (estimated)
Completion
Jul 1, 2029 (estimated)
Last update
Aug 31, 2026

Study contacts

Alexis Leonard, MD
Contact
referralinfo@stjude.org
888-226-4343
Alexis Leonard, MD
principal investigator · St. Jude Children's Research Hospital

Oversight

Data monitoring committee
Yes
FDA-regulated drug
Yes
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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