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RecruitingNCT07543757PRIMEUpdated Sep 4, 2026

Prostate Cancer Risk Identification in a Multi-Ethnic Cohort: a Prospective US-based Multi-center Validation Study of Proclarix

An observational study in Prostate Cancer Detection, sponsored by Sequenom, Inc.. Recruiting at 7 sites in United States. Open to male participants aged 40 Years to 75 Years. Per ClinicalTrials.gov, last updated 2026-09-04.

Sponsored by Sequenom, Inc. · Observational

Study type
Observational
Model
Case-only
Time perspective
Prospective
Enrollment
500
Ages
40 Years to 75 Years
Sex
Male
01

Study summary

The study is a prospective, multi-center, single cohort study involving up to 10 urological clinics in the US. After provision of informed consent and prior to the scheduled prostate biopsy (≤30 days), up to 20 mL of whole blood will be collected from each subject. The samples will be blinded and sent to Labcorp for evaluation using the Proclarix® assay. Prostate biopsy will be performed according to standard clinical practice of the urologist (systematic, targeted, or combined biopsy with a transrectal or transperineal approach with or without prior magnetic resonance imaging (MRI)). A minimum of 10 cores are required. Histopathological examination of the biopsy specimen will be performed according to the established local practice. A csPCa is defined as ISUP Grade Group ≥2 detected on biopsy. The assay results will be compared to the biopsy results.

Read the detailed description

Prostate cancer remains a leading cause of cancer-related morbidity and mortality in men worldwide, with significant disparities in incidence and outcomes across different ethnic groups with black men being affected by prostate cancer earlier, more frequently and with more aggressive disease. Current risk stratification tools often lack the precision needed to effectively assess and predict prostate cancer risk in diverse populations.

Proclarix® is a blood-based test that addresses the problem of prostate cancer (PCa) overdiagnosis by indicating the risk of clinically significant disease. It is comprised of two novel biomarkers, thrombospondin 1 (THBS1) and cathepsin D (CTSD), and is combined with total prostate-specific antigen (tPSA), free PSA (fPSA) and age. A software algorithm returns a risk score that can be used as an aid in the identification of clinically significant PCa (csPCa), defined as International Society of Urological Pathology (ISUP) Grade Group ≥ 2. Proclarix® has been developed and validated on 955 men, in majority of Caucasian background with 90% sensitivity, 43% specificity, 95% negative predictive value (NPV) and 25% positive predictive value (PPV). The validation performance established on German and Austrian patients has been fully confirmed in other European populations: the United Kingdom, Spain, Denmark, Italy and Switzerland.

To be used broadly, confirmation of Proclarix® performance in patients from diverse ethnic backgrounds requires further investigation.

02

Conditions studied

  • Prostate Cancer Detection

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Keywords

  • prostate cancer, screening
03

Who can participate

Ages eligible
40 Years to 75 Years
Sexes eligible
Male
Accepts healthy volunteers
No
Sampling method
Non-probability sample

Study population

  1. Previous diagnosis of prostate cancer;
  2. Treatment with a medication classified as a 5α-reductase-inhibitor at any time in the 6 months prior to study blood draw;
  3. Acute or chronic urinary tract infection within 3 months of the Screening Visit;
  4. Men with an indwelling catheter or intermittent self-catheterization;
  5. Digital Rectal Exam (DRE) or transurethral manipulation (e.g. cystoscopy, catheterization) the same day and prior to the Screening Visit blood draw;
  6. Men who had an invasive urological procedure for benign prostate hyperplasia/obstruction (e.g. transurethral resection of the prostate (TURP), Holmium Laser Enucleation of the Prostate (HoLEP), Thulium laser enucleation of the prostate (ThuLEP), aquablation, prostatic artery embolization, Rezum, laser vaporization, etc.) or biopsy of the prostate 6 months prior to the Screening Visit blood draw;
  7. Subject is not able to read and comprehend English or Spanish; or
  8. Previous enrollment in this protocol.

Inclusion criteria

  1. Subject is male ≥40 and ≤75 years of age at the time of enrollment;
  2. Subject provides a signed and dated informed consent;
  3. Subject has a SOC tPSA of 2-10 ng/mL inclusive within 30 days of the Screening Visit;

    1. Up to 100 subjects will have a SOC tPSA of 2-\<4 ng/mL
    2. A minimum of 400 subjects will have a SOC tPSA of 4-10 ng/mL
  4. Subject is scheduled to undergo a prostate biopsy within 30 days of the Screening Visit blood collection;
  5. Subject agrees to provide all diagnostic test results throughout the study; and
  6. Subject agrees to provide blood for Proclarix® and phi testing at the Screening Visit.

Exclusion criteria

Exclusion Criteria:

-

04

Study design

Observational model
Case-only
Time perspective
Prospective
Enrollment
500 participants (estimated)
Patient registry
No
Biospecimen retention
Samples without dna

Groups and cohorts

  • Prostate cancer suspected

    Subjects with an elevated PSA (2-10) planning to undergo prostate biopsy

    Diagnostic Test: Proclarix

Interventions

  • Diagnostic testProclarix

    Proclarix® is a blood-based test that addresses the problem of prostate cancer (PCa) overdiagnosis by indicating the risk of clinically significant disease. It is comprised of two novel biomarkers, thrombospondin 1 (THBS1) and cathepsin D (CTSD), and is combined with total prostate-specific antigen (tPSA), free PSA (fPSA) and age.

05

What researchers measure

Primary outcomes

  1. Assess the clinical performance of Proclarix® in a United States (US) cohort when compared to the biopsy results.

    Evaluate Proclarix®'s clinical performance, specifically NPV to predict the absence of csPCa on prostate biopsy.

    Time frame: From enrollment to the collection of prostate biopsy results at 90 days

Secondary outcomes

  1. Evaluate Proclarix's clinical ability to correlate with and add to the results of other diagnostic tests performed as standard of care (i.e., MRI, ultrasound, etc.)

    Compare study test results to SOC screening and diagnostic tests.

    Time frame: From enrollment to collection of biopsy results at 90 days

06

Study locations

7 of 7 sites recruiting
  • Om Research
    San Diego, California 92123, United States
    Recruiting
  • Idaho Urologic Institute
    Meridian, Idaho 83642, United States
    Recruiting
  • Graves Gilbert Clinic
    Bowling Green, Kentucky 42101, United States
    Recruiting
  • Chesapeake Urology Associates
    Hanover, Maryland 21076, United States
    • Barbara Kyei · Contact · bkyei@chesuro.com · 410-760-9400
    • Kaiser Robertson, MD · Principal investigator
    Recruiting
  • Chesapeake Urology Associates
    Towson, Maryland 21204, United States
    Recruiting
  • Crystal Run Healthcare
    Middletown, New York 10941, United States
    Recruiting
  • Carolina Urologic Research Center
    Myrtle Beach, South Carolina 29572, United States
    Recruiting
07

References and documents

Individual participant data

Plan to share: No

No publications or documents are linked to this record.

08

Registry details

Key details

Study ID
NCT07543757
Lead sponsor
Sequenom, Inc.
Collaborators
Labcorp Corporation of America Holdings, Inc
Responsible party
Sponsor
First posted
Apr 22, 2026
Start date
Apr 3, 2026
Primary completion
Mar 31, 2027 (estimated)
Completion
Mar 31, 2027 (estimated)
Last update
Sep 4, 2026

Study contacts

Senior Clinical Trial Manager
Contact
clinicalaffairsSD@labcorp.com
858-202-2000
Neil Shore, MD
principal investigator · Carolina Urologic Research Center

Oversight

FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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