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RecruitingNCT07362446PROTECT-MIUpdated Sep 14, 2026

Prevention of Reperfusion Injury Outcomes Through Effective Cardioprotection Targeting Myocardial Infarction

A Phase 2 interventional study of Xolatryp and Placebo in Myocardial Infarction, Reperfusion Injury and AMI, sponsored by Nyrada Pty Ltd. Recruiting at 8 sites in Australia. Open to participants aged 40 Years to 75 Years. Per ClinicalTrials.gov, last updated 2026-09-14.

Sponsored by Nyrada Pty Ltd · Phase 2, Interventional, and Treatment

Phase
Phase 2
Study type
Interventional
Enrollment
300
Allocation
Randomized
Ages
40 Years to 75 Years
Sex
All
01

Study summary

This study is open to adults with ST elevation myocardial infarction (heart attack) undergoing primary percutaneous coronary intervention (PCI). The purpose of this study is to determine whether a medicine called Xolatryp is safe and effective in improving cardiac outcomes. One dose of Xolatryp will be tested in this study.

Participants are put into two groups randomly, which means by chance. One group receives a single 6-hour continuous intravenous infusion of Xolatryp and one group receives placebo. Participants are in the study for about 30 days.

Placebo infusion looks like Xolatryp but do not contain any medicine. Participants are followed up via telephone and there is one visit to the study site on day 30.

Heart health is assessed based on the analysis of blood samples, which are collected at the study site, via electrocardiogram (ECG), echocardiogram and cardiac magnetic resonance (CMR) imaging. At the end of the study, the results are compared between the two groups. During the study, the doctors also regularly check the general health of the participants.

02

Conditions studied

  • Myocardial Infarction
  • Reperfusion Injury
  • AMI
  • STEMI (ST Elevation MI)

Keywords

  • STEMI
  • PCI
03

Who can participate

Ages eligible
40 Years to 75 Years
Sexes eligible
All
Accepts healthy volunteers
No

Inclusion criteria

  • Have provided informed consent.
  • Male patients aged 40 to 75 years of age.- Female patients aged 55 to 75 years of age, or women aged 40 to 55 years that have no possibility of being pregnant.
  • Patient presents with first-time STEMI, scheduled to undergo primary PCI within 6 h of symptom onset and anticipated door to balloon time \< 2 h.
  • In combination with symptoms consistent with acute MI, patient must demonstrate ST-elevation at the J-point in two contiguous leads.
  • Hemodynamically stable including: systolic BP ≥ 90 mmHg, HR 50-120 bpm.
  • Killip Class I or II.
  • Oxygen saturation ≥ 92% on room air or low-flow oxygen.
  • No ongoing VT/VF at enrolment.
  • Male participants with female partners of child-bearing potential must be ready and able to use highly effective methods of birth control for at least 7 days following IP administration.

Exclusion criteria

Exclusion Criteria:

  • History or ECG evidence of myocardial infarction or cardiomyopathy.
  • Prior major cardiac surgery, including but not limited to coronary artery bypass graft surgery (CABG).
  • Known contraindication to CMR (e.g. pacemakers, cochlear implants, aneurism clips, claustrophobia, allergy to contrast medium).
  • History of clinically significant renal impairment requiring dialysis or an estimated glomerular filtration rate \<30 mL/min.
  • Estimated or known body weight \< 50 kg, > 120 kg at screening.
  • Concurrent enrolment in another investigational device or drug trial, or less than 30 days or 5 half-lives of investigational device or drug (whichever is longer), since ending another investigational device or drug trial(s) or receiving other investigational treatment(s). Patients who are participating in non-interventional, purely observational trials can be included.
  • Life expectancy of less than 1 year due to non-cardiac pathology in the opinion of the Investigator.
  • Any condition or significant clinical abnormality identified at the time of screening that in the judgment of the Investigator or any sub-Investigator would preclude safe completion of the study.
  • Known history of hypersensitivity to the investigational drug, or excipients, or do not want to be exposed to soy or egg (including products and derivatives).
04

Study design

Phase
Phase 2
Primary purpose
Treatment
Allocation
Randomized
Intervention model
Parallel assignment
Masking
Quadruple (Participant, Care provider, Investigator, Outcomes assessor)
Enrollment
300 participants (estimated)

Study arms

  • Experimental
    Xolatryp intravenous infusion

    After randomization, patients receive primary PCI and standard therapy. Patients assigned to the experimental arm also receive Xolatryp, administered as a single, continuous intravenous infusion (i.v.) for 6 hours.

    Drug: Xolatryp

  • Placebo comparator
    Placebo intravenous infusion

    After randomization, patients receive primary PCI and standard therapy. Patients assigned to the placebo arm also recieve a placebo comparator, administered as a single, continuous intravenous (i.v.) infusion for 6 hours.

    Drug: Placebo

Interventions

  • DrugXolatryp

    Patients assigned to the treatment arm recieve Xolatryp administered as a continuous intravenous (i.v) infusion for 6 hours.

  • DrugPlacebo

    Patients assigned to the placebo comparator arm receive 0.1% of 20% Intralipid in 0.9% normal saline, administered via continuous intravenous (i.v.) infusion for 6 hours.

05

What researchers measure

Primary outcomes

  1. Incidence of Adverse Events

    Incidence of adverse events (AE) and serious adverse events (SAE) overall. Incidence of AEs and SAEs deemed related to Xolatryp. Incidence of AEs and SAEs deemed cardiac related.

    Time frame: From enrollment up to and including follow-up assessments on Day 30 (end of study)

Secondary outcomes

  1. Plasma concentration of Xolatryp during the infusion

    Blood sample(s) taken at 4 hours post the start of infusion

    Time frame: Blood samples will be taken for pharmacokinetic (PK) assessment at 4 hours after start of infusion. Where feasible, a sample should be taken at 10 minutes into the infusion.

  2. ST-segment elevation resolution

    Comparison between treatment groups of ST-segment elevation resolution calculated from pre-PCI ECG and first post procedural ECG

    Time frame: pre-PCI ECG and first post procedural ECG

  3. Cardiac Injury Biomarkers

    Plasma Troponin I levels calculated as area under the curve (AUC) over 48 hours post PCI

    Time frame: 48 hours post PCI

  4. Incidence of Arrhythmias

    Comparison of number of arrhythmias of interest recorded on telemetry (48 hours) between patients treated with Xolatryp vs Placebo. Arrhythmias of interest are sustained ventricular tachycardia (VT), non-sustained ventricular tachycardia (NSVT), and high degree- atrioventricular (AV) block.

    Time frame: Telemetry to 48 hours

Other outcomes

  1. Cardiac Infarct Size

    Volumetric quantification comparison between treatment groups of cardiac infarct size parameters: Acute myocardial infarct size indexed to left ventricular mass, and Myocardial Salvage Index (MSI).

    Time frame: Day 5 (+/- 2 days)

  2. Left Ventricular End-diastolic volume

    Left Ventricular End-diastolic volume measurement from CMR will be used to assess cardiac function on Day 5 (+/- 2 days)

    Time frame: Day 5

  3. Patient Reported Outcomes (PRO) Questionnaire

    36-Item Short Form Health Survey (SF-36)

    Time frame: Day 30 (end of study)

  4. Left Ventricular Ejection fraction

    Left ventricular ejection fraction measurement from echocardiogram will be used to assess cardiac function prior to discharge and 30 days post primary PCI

    Time frame: Day 2 and Day 30

  5. Left Ventricular End-systolic Volume

    Left Ventricular Volume measurement from echocardiogram will be used to assess cardiac function prior to discharge and 30 days post primary PCI

    Time frame: Day 2 and Day 30

  6. Left Ventricular Function

    Left Ventricular Function measurement from echocardiogram will be used to assess cardiac function prior to discharge and 30 days post primary PCI

    Time frame: Day 2 and Day 30

  7. Fractional shortening

    Fractional shortening will be assessed via echocardiogram prior to discharge and at Day 30.

    Time frame: Day 2 and 30

06

Study locations

3 of 8 sites recruiting
  • Nepean Hospital
    Kingswood, New South Wales 2747, Australia
    Recruiting
  • Liverpool Hospital
    Liverpool, New South Wales 2170, Australia
    Not yet recruiting
  • Royal Adelaide Hospital
    Adelaide, South Australia 5000, Australia
    Not yet recruiting
  • Northern Health
    Epping, Victoria 3076, Australia
    Recruiting
  • Peninsula University Hospital
    Frankston, Victoria 3199, Australia
    Not yet recruiting
  • Sunshine Hospital
    Saint Albans, Victoria 3021, Australia
    Not yet recruiting
  • Sir Charles Gairdner Hospital
    Nedlands, Western Australia 6009, Australia
    Recruiting
  • Royal Perth Hospital
    Perth, Western Australia 6000, Australia
    Not yet recruiting
07

Registry details

Key details

Study ID
NCT07362446
Lead sponsor
Nyrada Pty Ltd
Responsible party
Sponsor
First posted
Jan 23, 2026
Start date
Jul 2, 2026
Primary completion
Jun 30, 2027 (estimated)
Completion
Sep 30, 2027 (estimated)
Last update
Sep 14, 2026

Study contacts

Alexandra Suchowerska Director, Clinical Operations and Regulatory Affairs, PhD
Contact
Protect-MI@Nyrada.com
+61 294-983-390

Oversight

Data monitoring committee
No
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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