An observational study in Evaluate the Fluorescence of Natural Tooth Following Dehydration, sponsored by Marmara University. Active, not recruiting at 1 site in Turkey (Türkiye). Open to participants aged 18 Years to 35 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2025-12-22.
Sponsored by Marmara University · Observational
A cheek retractor will be placed to ensure consistent isolation throughout the procedure. Dehydration will be assessed at baseline, at the 5th minute, and at the 10th minute using three different evaluation techniques. At each time point, digital photographs will be captured under two distinct fluorescence light sources to document changes visually. In addition, fluorescence images will be obtained using the QLF (Quantitative Light-Induced Fluorescence) device to provide objective, quantitative data. All images will be recorded and analyzed digitally to evaluate dehydration-related changes in fluorescence. The aim of our study is to clinically evaluate the changes in the fluorescence properties of natural tooth following dehydration.
According to the power analysis, the required number of teeth was 69, with 95% statistical power and a 5% alpha error level. The study will be conducted at the Clinic of the Department of Restorative Dentistry, Marmara University Faculty of Dentistry. Individuals with caries-free teeth, no enamel defects, no restorations on tooth surfaces, no visible plaque, and adequate cognitive ability to read and understand the informed consent form will be included in the study. Those with teeth exhibiting discoloration or with systemic conditions or medication use that may cause structural changes on the tooth surfaces will be excluded. Maxillary and mandibular anterior teeth will be evaluated.
A cheek retractor will be placed in the patients' mouths, and baseline, 5th-minute, and 10th-minute dehydration will be evaluated using three different techniques. Digital photographs will be taken at baseline and post-procedure under two different fluorescence light sources (Ashur plaque indicator (405 nm), and Led lights (365 nm)), and the images obtained using the QLF (Quantitative Light-Induced Fluorescence- 405 nm) device will be recorded. Analyses will be carried out digitally. Images will be captured with a Sony Alpha 7iii (Sony, Japan) digital camera equipped with a 90-mm macro lens. Camera settings will be adjusted for the LED lights (1/125, f/22, ISO 8000) and for the Ashur device (1/125, f/22, ISO 25600). To ensure standardization, the LED lights will be stabilized on the flash brackets at a 45° angle on both sides, and all images will be captured in a dark room. QLF images will be obtained with its own camera. The photographs and the QLF images will be analyzed using Adobe Photoshop software, and color changes will be determined based on ∆E values. For each method ∆E values will be calculated individually, there will be 3 different ∆E values (baseline and 5th-minute, baseline and 10th-minute, 5th-minute and 10th-minute). For ∆E calculations, the CIEDE2000 formula will be used, employing the measured L*, a*, b* and L*, c*, h* values obtained from baseline, 5th-minute and 10th-minute images. For the digital measurements, the intraobserver variability will be assessed, and after obtaining a high level of agreement, the study will be initiated. In statistical analyses, normality will be assessed using the Shapiro-Wilk test; repeated-measures ANOVA will be used if normal distribution is present, and the Friedman test will be applied if normality is not observed. A significance level of p\<0.05 will be accepted.
The aim of this clinical study is to clinically evaluate the changes in the fluorescence properties of natural teeth following dehydration.
The hypotheses of the study (H1) are as follows:
201 studies on the registry are indexed under Dehydration; 28 are open to participants now.
This study's planned enrollment of 10 is below the median of 105 across 46 observational studies indexed under Dehydration.
Browse Dehydration studies →Marmara University is the lead sponsor of 599 studies on the registry; 138 are open to participants now.
Counted across the registry records on this site, refreshed daily.
Individuals with caries-free teeth, no enamel defects, no restorations on tooth surfaces, no visible plaque, and adequate cognitive ability to read and understand the informed consent form will be included in the study. Those with teeth exhibiting discoloration or with systemic conditions or medication use that may cause structural changes on the tooth surfaces will be excluded. Maxillary and mandibular anterior teeth will be evaluated.
Go through the criteria one by one — yes, no or not sure — and take the list to your doctor or the study team. Kept in this browser only.
Exclusion Criteria:
Underlined terms in the criteria open these too. Explained by clinicaltrials.gg, not part of the registry record. The study team decides who can take part.
Before you join, the study team explains what taking part involves — its purpose, what happens, the risks and possible benefits — and answers your questions, so you can decide freely. Consent is usually recorded on a form (the informed consent form, ICF) signed by you or, where the law allows, by someone legally authorised to decide for you.
You can keep asking questions during the study, you are told of new information that matters, and you can choose to leave at any time.
Informed consent
Before you join, the study team explains what taking part involves — its purpose, what happens, the risks and possible benefits — and answers your questions, so you can decide freely. Consent is usually recorded on a form (the informed consent form, ICF) signed by you or, where the law allows, by someone legally authorised to decide for you.
You can keep asking questions during the study, you are told of new information that matters, and you can choose to leave at any time.
Explained by clinicaltrials.gg, not part of the registry record. The study team decides who can take part.
Dehydration of teeth will be assessed at baseline, at the 5th minute, and at the 10th minute using three different evaluation techniques.
Diagnostic Test: Digital photograph with Ashur plaque indicator · Diagnostic Test: Digital photographs with the Led lights (365 nm) · Diagnostic Test: The QLF (Quantitative Light-Induced Fluorescence- 405 nm) images
Digital photographs will be taken at baseline and post-procedure under the fluorescence light source (Ashur plaque indicator (405 nm). Images will be captured with a Sony Alpha 7iii (Sony, Japan) digital camera equipped with a 90-mm macro lens. Camera settings will be adjusted for the Ashur device (1/125, f/22, ISO 25600). To ensure standardization all images will be captured in a dark room.
Digital photographs will be taken at baseline and post-procedure under the fluorescence light source (Led lights (365 nm). Images will be captured with a Sony Alpha 7iii (Sony, Japan) digital camera equipped with a 90-mm macro lens. Camera settings will be adjusted for the LED lights (1/125, f/22, ISO 8000). To ensure standardization, the LED lights will be stabilized on the flash brackets at a 45° angle on both sides, and all images will be captured in a dark room.
The images obtained using the QLF (Quantitative Light-Induced Fluorescence- 405 nm) device will be recorded. QLF images will be obtained with its own camera.
Digital photograph with Ashur plaque indicator
Digital photographs will be taken at baseline and post-procedure under the fluorescence light source (Ashur plaque indicator (405 nm). Images will be captured with a Sony Alpha 7iii (Sony, Japan) digital camera equipped with a 90-mm macro lens. Camera settings will be adjusted for the Ashur device (1/125, f/22, ISO 25600). To ensure standardization all images will be captured in a dark room.
Time frame: baseline
Digital photographs with the Led lights (365 nm)
Digital photographs will be taken at baseline and post-procedure under the fluorescence light source (Led lights (365 nm). Images will be captured with a Sony Alpha 7iii (Sony, Japan) digital camera equipped with a 90-mm macro lens. Camera settings will be adjusted for the LED lights (1/125, f/22, ISO 8000). To ensure standardization, the LED lights will be stabilized on the flash brackets at a 45° angle on both sides, and all images will be captured in a dark room.
Time frame: baseline
The QLF (Quantitative Light-Induced Fluorescence- 405 nm) images
The images obtained using the QLF (Quantitative Light-Induced Fluorescence- 405 nm) device will be recorded. QLF images will be obtained with its own camera. All images will be captured in a dark room.
Time frame: baseline
Digital photograph with Ashur plaque indicator
Digital photographs will be taken at baseline and post-procedure under the fluorescence light source (Ashur plaque indicator (405 nm). Images will be captured with a Sony Alpha 7iii (Sony, Japan) digital camera equipped with a 90-mm macro lens. Camera settings will be adjusted for the Ashur device (1/125, f/22, ISO 25600). To ensure standardization all images will be captured in a dark room.
Time frame: at 5th minute
Digital photographs with the Led lights (365 nm)
Digital photographs will be taken at baseline and post-procedure under the fluorescence light source (Led lights (365 nm). Images will be captured with a Sony Alpha 7iii (Sony, Japan) digital camera equipped with a 90-mm macro lens. Camera settings will be adjusted for the LED lights (1/125, f/22, ISO 8000). To ensure standardization, the LED lights will be stabilized on the flash brackets at a 45° angle on both sides, and all images will be captured in a dark room.
Time frame: at 5th minute
The QLF (Quantitative Light-Induced Fluorescence- 405 nm) images
The images obtained using the QLF (Quantitative Light-Induced Fluorescence- 405 nm) device will be recorded. QLF images will be obtained with its own camera. All images will be captured in a dark room.
Time frame: at 5th minute
Digital photograph with Ashur plaque indicator
Digital photographs will be taken at baseline and post-procedure under the fluorescence light source (Ashur plaque indicator (405 nm). Images will be captured with a Sony Alpha 7iii (Sony, Japan) digital camera equipped with a 90-mm macro lens. Camera settings will be adjusted for the Ashur device (1/125, f/22, ISO 25600). To ensure standardization all images will be captured in a dark room.
Time frame: at 10th minute
Digital photographs with the Led lights (365 nm)
Digital photographs will be taken at baseline and post-procedure under the fluorescence light source (Led lights (365 nm). Images will be captured with a Sony Alpha 7iii (Sony, Japan) digital camera equipped with a 90-mm macro lens. Camera settings will be adjusted for the LED lights (1/125, f/22, ISO 8000). To ensure standardization, the LED lights will be stabilized on the flash brackets at a 45° angle on both sides, and all images will be captured in a dark room.
Time frame: at 10th minute
The QLF (Quantitative Light-Induced Fluorescence- 405 nm) images
The images obtained using the QLF (Quantitative Light-Induced Fluorescence- 405 nm) device will be recorded. QLF images will be obtained with its own camera. All images will be captured in a dark room.
Time frame: at 10th minute
Plan to share: Yes — Data may be shared after the publication of the study
Supporting information: Study protocol, Statistical analysis plan, Informed consent form, Clinical study report
No publications or documents are linked to this record.
Ran from Apr 2025 to primary completion in Jun 2025, 1 month.
Of 19 observational studies under Dehydration, half reached primary completion within 18 months of starting (the median), counting those still running; a quarter within 6 and three quarters within 30. This study's 1 month is below that.
How these figures are worked out, and which studies are left out of them.
This study is active, not recruiting, as verified in Dec 2025. You cannot join it, but the record below documents what was studied.
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