An interventional study of Exercise: 80% 1 RM with 2 minutes break and Exercise: 80% 1RM with1 minute break in BDNF, Irisin and Resistance Exercise, sponsored by University of Ljubljana. Active, not recruiting at 1 site in Slovenia. Open to male participants aged 18 Years to 30 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2025-12-16.
Sponsored by University of Ljubljana · Not applicable, Interventional, and Basic science
The aim of this study is to investigate different resistance training loads on BNDF and irisin levels in platelets, blood serum and plasma.
In this study, the investigators will examine how three resistance training protocols acutely affect BDNF levels (in serum, plasma, platelet count, and BDNF per platelet) and irisin levels in blood (plasma). The resistance training protocols will differ in intensity, rest intervals between sets and repetition execution. Blood samples will be collected from participants at four time points: before the start of the protocol, 5 minutes after completion of the final set, 1 hour after completion of the final set, and 3 hours after completion of the final set. In addition, blood lactate levels will be monitored at the same time points. Heart rate will also be recorded (immediately before the start of the protocol and 30 seconds after completion of the final set). A control protocol will also be implemented, in which blood will be collected at the same time points. Data will be collected across five visits to the kinesiology laboratory at the University of Ljubljana, Faculty of Sport. Each visit will last approximately 4 hours, except for the first visit where 1RM will be measured.
University of Ljubljana is the lead sponsor of 42 studies on the registry; 12 are open to participants now.
Counted across the registry records on this site, refreshed daily.
Exclusion Criteria:
All participants will do 1 control protocol and 3 resistance exercises protocols. Order of protocols will be randomized.
Other: Exercise: 80% 1 RM with 2 minutes break · Other: Exercise: 80% 1RM with1 minute break · Other: Exercise: accenuated eccentric exercise · Other: Control
Participant will do resistance exercise at 80% 1RM with 2 minutes break, 4 sets of each exercise. Included exercises are lat pull, bench press and leg press.
Also known as: resistance exercise
Participant will do resistance exercise at 80% 1RM with 1 minutes break, 4 sets of each exercise. Included exercises are lat pull, bench press and leg press.
Also known as: resistance exercise
Participant will do accenuated eccentric resistance exercise at 80% 1RM in concetric part of movement and 110% 1RM in eccentric part of movement with 2 minutes break, 4 sets of each exercise. Included exercises are lat pull, bench press and leg press.
Timeline of events will be same as in exercise protocol (blood withdrawal and Stroop test), except participants will not do any kind of exercise protocol, instead they will be seating.
plasma BDNF
BDNF will be determined in blood plasma with ELISA kit.
Time frame: pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.
Irisin
Irisin will be measured in blood plasma.
Time frame: pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.
serum BDNF
BDNF will be determined in blood serum with ELISA kit.
Time frame: pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.
Platelet count
Platelet count in blood will be done.
Time frame: pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.
BDNF per platelet
The amount of BDNF per platelet will be calculated.
Time frame: pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.
Lactate
Lactate will be measured in blood.
Time frame: pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.
Stroop test
Stroop test will be used as an assessment of cognitive functions. The number of read word will be assessed.
Time frame: pre, 15 minutes post exercise.
This study is active, not recruiting, as verified in Dec 2025. You cannot join it, but the record below documents what was studied.
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University of Ljubljana