Investigation of the Interplay Between Local Oral Inflammation, Microbiota and Systemic Immune Responses.

1. Males and females, between 18-65 years of age;
2. Availability for the duration of the study;
3. ASA 1 Good general health (absence of any self-reported systemic disease or condition that, in the opinion of the Principal Investigator, might constitute a risk to the subject while participating in the study. Examples include heart problems, valve/hip replacements, etc);
4. Willingness to provide information related to their medical history;
5. Minimum of 20 uncrowned permanent natural teeth (excluding third molars and crowns);
6. Informed Consent Form signed.

Exclusion Criteria:

1. Subjects with more that 2 sites with pocket depth of > 4 mm and those presenting purulent exudate, tooth mobility, and/or extensive loss of periodontal attachment or alveolar bone;
2. Oral pathology or a history of allergy to testing products;
3. Subject using anticonvulsants, antihistamines, antidepressants, sedatives, tranquilizers, anti-inflammatory medication or daily analgesics within one month prior to the start of the study or scheduled to start such intake during the course of the study; to be defined by the dental examiner;
4. Subject participating in any other clinical study;
5. Subject pregnant or breastfeeding;
6. Subject allergic to oral care products and personal care consumer products
7. Extended use of antibiotics or therapeutic mouthwash (eg: Chlorhexidine) any time during the three months prior to entry into the study;
8. Ongoing use of medications known to affect the gingival tissues (i.e. calcium channel blockers, phenytoin, cyclosporine)
9. Current smokers (or users of tobacco and vaping products) and subjects with a history of alcohol or drug abuse;
10. An existing medical condition which prohibits eating or drinking for periods up to 4 hours.
11. Individuals with orthodontic bands or removable partial dentures.
12. Dental prophylaxis in the previous 3 months prior to the baseline examination