An observational study in Inflammation, Iron Deficiency Anemias and Red Blood Cell, sponsored by Poznan University of Physical Education. Completed at 1 site in Poland. Open to male participants aged 18 Years to 24 Years. Per ClinicalTrials.gov, last updated 2025-03-20.
Sponsored by Poznan University of Physical Education · Observational
The data of 16 trained men from the Polish Youth National Rowing. All athletes presented as medically fit, with no underlying health problems. In this cohort, baseline serum ferritin levels were considered, and the athletes were divided into two groups:
The rowing test was performed at the beginning of the preparatory phase of the annual training cycle (April), which is characterized by high volume and training load. After a 5-min of individual warm-up, the athletes performed a 2000-m exercise test on a rowing ergometer (Concept II, USA). Blood samples took from the cubital vein at three time points: before stress test (pre-exercise), one minute after the end of the test (post-exercise), and after a 1-hour recovery period (1h recovery).
Measurements ferritin, transferrin, hepcidin and interleukin-6 (IL-6) levels were determined using ELISA kits (SunRed Biotechnology Company). The iron levels were determined on the SPECTROstar Nano reader.
The data of 16 trained men from the Polish Youth National Rowing. All athletes presented as medically fit, with no underlying health problems. In this cohort, baseline serum ferritin levels were considered, and the athletes were divided into two groups:
The rowing test was performed at the beginning of the preparatory phase of the annual training cycle (April), which is characterized by high volume and training load. After a 5-min of individual warm-up, the athletes performed a 2000-m exercise test on a rowing ergometer (Concept II, USA). Blood samples took from the cubital vein at three time points: before stress test (pre-exercise), one minute after the end of the test (post-exercise), and after a 1-hour recovery period (1h recovery).
Measurement: All determined parameters will be measured with the available equipment in the ZWKF laboratory in Gorzów Wielkopolski and using commercial assay kits. Measurements will be performed by the project contractors.
Polyethylene tubes (2.7ml) containing dipotassium ethylenediaminetetraacetic acid (EDTAK2) anticoagulant will be used for the following tests:
(a) complete blood count (7 parameters) determined on the MYTHIC 18 hematology analyzer (Orphee Medical, Geneva, Switzerland). Red blood cell indices: RBC (Red Blood Cells), HGB (Hemoglobin), HCT (Hematocrit), MCV (Mean Corpuscular Volume), MCH (Mean Corpuscular Hemoglobin), MCHC (Mean Corpuscular Hemoglobin Concentration), RDW (Red Blood cells Distribution Width).
Polyethylene clotting activator tubes (9 ml) will be centrifuged to separate the morphotic elements from the serum using a centrifuge (3000 rpm for 10 min). The serum will be pipetted into several Eppendorf tubes, which will then be frozen (temp. -80 °C). All the following biochemical parameters will be determined from the extracted serum:
using the ELISA method by the test manufacturer's instructions. The designations include the flowing parameters:
Youth National Rowing Team of Poland The athletes' diets were completely analyzed before each exercise test by a nutritionist. Participants, with the help of a dietician who was available during meals on test days, filled out food diaries. The amount of energy, protein, carbohydrates, fats and fiber were then analyzed using a commercially available program.
The experiment was conducted in accordance with the Declaration of Helsinki. The study protocol was approved by the Ethical Committee of the Medical University of Poznan (decision no. 685/23 in 2023).
644 studies on the registry are indexed under Anemia, Iron-Deficiency; 105 are open to participants now.
This study's enrollment of 16 is below the median of 200 across 113 observational studies indexed under Anemia, Iron-Deficiency.
Browse Anemia, Iron-Deficiency studies →Poznan University of Physical Education is the lead sponsor of 90 studies on the registry; 27 are open to participants now.
Counted across the registry records on this site, refreshed daily.
Youth National Rowing Team of Poland
Exclusion Criteria:
Ferritin
Concentration of ferritin \[ng/mL\]. ELISA method by the test manufacturer's instructions
Time frame: At rest (before the test), directly after the test, and after a 1-hour recovery period.
Iron
The iron level \[µg/dL\] will be determined using the colorimetric method on the SPECTROstar Nano reader.
Time frame: At rest (before the test), directly after the test, and after a 1-hour recovery period.
Interleukin-6 (Il-6)
Concentration of IL-6 \[ng/L\]. ELISA method by the test manufacturer's instructions
Time frame: At rest (before the test), directly after the test, and after a 1-hour recovery period.
Hepcidin
Concentration of hepcidin \[ng/mL\]. ELISA method by the test manufacturer's instructions
Time frame: At rest (before the test), directly after the test, and after a 1-hour recovery period.
Transferrin
Concentration of transferrin \[mg/mL\]. ELISA method by the test manufacturer's instructions
Time frame: At rest (before the test), directly after the test, and after a 1-hour recovery period.
The Blood morphology - RBC (Red Blood Cells)
RBC (Red Blood Cells) \[106 × µL-1\] determined on the MYTHIC 18 hematology analyzer (Orphee Medical, Geneva, Switzerland).
Time frame: At rest (before the test), directly after the test, and after a 1-hour recovery period.
The Blood morphology- HGB (Hemoglobin)
HGB (Hemoglobin) \[g/dL\] determined on the MYTHIC 18 hematology analyzer (Orphee Medical, Geneva, Switzerland).
Time frame: At rest (before the test), directly after the test, and after a 1-hour recovery period.
The Blood morphology- HCT (Hematocrit)
HCT (Hematocrit) \[%\]determined on the MYTHIC 18 hematology analyzer (Orphee Medical, Geneva, Switzerland).
Time frame: At rest (before the test), directly after the test, and after a 1-hour recovery period.
The Blood morphology- MCV (Mean Corpuscular Volume)
MCV (Mean Corpuscular Volume)\[fl\] determined on the MYTHIC 18 hematology analyzer (Orphee Medical, Geneva, Switzerland).
Time frame: At rest (before the test), directly after the test, and after a 1-hour recovery period.
The Blood morphology- MCH (Mean Corpuscular Hemoglobin)
MCH (Mean Corpuscular Hemoglobin) \[pg\] determined on the MYTHIC 18 hematology analyzer (Orphee Medical, Geneva, Switzerland).
Time frame: At rest (before the test), directly after the test, and after a 1-hour recovery period.
The Blood morphology- MCHC (Mean Corpuscular Hemoglobin Concentration)
MCHC (Mean Corpuscular Hemoglobin Concentration) \[g x dL-1\] determined on the MYTHIC 18 hematology analyzer (Orphee Medical, Geneva, Switzerland).
Time frame: At rest (before the test), directly after the test, and after a 1-hour recovery period.
The Blood morphology- RDW (Red Blood cells Distribution Width)
RDW (Red Blood cells Distribution Width) \[%\] determined on the MYTHIC 18 hematology analyzer (Orphee Medical, Geneva, Switzerland).
Time frame: At rest (before the test), directly after the test, and after a 1-hour recovery period.
Antropometric characteristic - height
Prior to the exercise test, the investigators measured the anthropometric parameters, including height (Seca 213 Hamburg, Deutschland) \[cm\]
Time frame: Day 1 after overall fast
Antropometric characteristic - weight
Prior to the exercise test, the investigators measured the anthropometric parameters, including weight (Tanita BC 418 MA, Tanita Corporation, Tokyo, Japan) \[kg\]
Time frame: Day 1 after overall fast
Food record - energy
Participants will prepare a food record. The results will be calculated using the dietetykpro program: energy \[kcal\]
Time frame: Day before the Day 1, in the morning at Day 1
Food record - protein
Participants will prepare a food record. The results will be calculated using the dietetykpro program: protein \[g\]
Time frame: Day before the Day 1, in the morning at Day 1
Food record - carobhydrates
Participants will prepare a food record. The results will be calculated using the dietetykpro program: carbohydrates \[g\]
Time frame: Day before the Day 1, in the morning at Day 1
Food record - fiber
Participants will prepare a food record. The results will be calculated using the dietetykpro program: fiber \[g\]
Time frame: Day before the Day 1, in the morning at Day 1
Food record - fat
Participants will prepare a food record. The results will be calculated using the dietetykpro program: fat \[g\]
Time frame: Day before the Day 1, in the morning at Day 1
Plan to share: No
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This study is completed, as verified in Mar 2025. You cannot join it, but the record below documents what was studied.
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Poznan University of Physical Education