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RecruitingNCT06723925Updated Mar 12, 2026

Neonatal Screening of Biotinidase Deficiency: Genotype-phenotype Correlation and Clinical Follow-up

An observational study in Biotinidase Deficiency, sponsored by IRCCS Azienda Ospedaliero-Universitaria di Bologna. Recruiting at 1 site in Italy. Open to participants aged Up to 36 Months. Per ClinicalTrials.gov, last updated 2026-03-12.

Sponsored by IRCCS Azienda Ospedaliero-Universitaria di Bologna · Observational

Study type
Observational
Model
Cohort
Time perspective
Other
Enrollment
180
Ages
Up to 36 Months
Sex
All
01

Study summary

Retro-prospective, single-centre, observational study conducted at the Endocrine-Metabolic Diseases Center of the Pediatrics Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Italy.

The study involves children born in Emilia-Romagna region, Italy, from January 2016 to December 2020 with biotinidase deficiency identified through Neontal Screening at the Endocrine-Metabolic Diseases Center of the Pediatrics Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Italy. The primary aim of this study is to assess the incidence of biotinidase decificiency in this cohort of patients and the possible correlation between the genotype and the biochemical and clinical phenotype of this cohort of patients.

Read the detailed description

The study consists of the retrospective collection and analysis of clinical, biochemical and genetic data of pediatric patients who were taken in charge for Biotinidase Deficiency at the Endocrine-Metabolic Diseases Center of the Pediatrics Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Italy, following Neonatal Screening positivity.

For this cohort of patients, a clinical evaluation is planned annually after the diagnosis of Biotinidase Deficiency for the identification of possible long-term complications. A clinical follow-up of at least 36 months is expected.

According to clinical practice, parents of pediatric patients with Biotinidase Deficiency identified through Neonatal Screening will undergo molecular genetic analysis for specific familial mutations of the BTD gene, but will not be followed up.

02

Conditions studied

  • Biotinidase Deficiency

Keywords

  • Biotinidase Deficiency
03

Who can participate

Ages eligible
Up to 36 Months
Sexes eligible
All
Accepts healthy volunteers
No
Sampling method
Non-probability sample

Study population

Pediatric patients with Biotinidase Deficiency (and their parents) identified through Neonatal Screening from January 2016 to December 2020 at the Endocrine-Metabolic Diseases Center of the Pediatrics Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Italy.

Inclusion criteria

FOR PEDIATRIC PATIENTS

  • Neonatal Screening test result of Residual biotinidase Enzyme Activity \<50% carried out from January 2016 to December 2019 at the Endocrine-Metabolic Diseases Center of the Pediatrics Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Italy;
  • Neonatal Screening test result of Residual biotinidase Enzyme Activity \<30% carried out from January 2020 to December 2020 at the Endocrine-Metabolic Diseases Center of the Pediatrics Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Italy;
  • Obtaining informed consent from parents or legal guardian of pediatric patients.

FOR PARENTS

  • Being a parent of a paediatric patient enrolled in the study;
  • Availability of parental data;
  • Obtaining informed consent.

Exclusion criteria

EXCLUSION CRITERIA:

  • Subjects with known chromosomal abnormalities or complex syndromes.
04

Study design

Observational model
Cohort
Time perspective
Other
Enrollment
180 participants (estimated)
Patient registry
No
05

What researchers measure

Primary outcomes

  1. BTD gene mutation

    allele1, allele2 mutations

    Time frame: baseline

  2. Residual biotinidase Enzymatic Activity

    percentage %

    Time frame: baseline

  3. Biotin replacement therapy

    mg/die

    Time frame: baseline

  4. Presence of Sintomatology

    ocular, dermatological, neuropsychiatric symptoms

    Time frame: annually after the diagnosis of Biotinidase Deficiency up to 3 yaers

06

Study locations

1 of 1 sites recruiting
  • IRCCS Azienda Ospedaliero-Universitaria di Bologna
    Bologna, Bologna 40138, Italy
    Recruiting
07

Registry details

Key details

Study ID
NCT06723925
Lead sponsor
IRCCS Azienda Ospedaliero-Universitaria di Bologna
Responsible party
Rita Ortolano (MD, IRCCS Azienda Ospedaliero-Universitaria di Bologna) — Principal investigator
First posted
Dec 9, 2024
Start date
Apr 21, 2021
Primary completion
Oct 31, 2026 (estimated)
Completion
Dec 31, 2026 (estimated)
Last update
Mar 12, 2026

Study contacts

Rita Ortolano, MD
Contact
rita.ortolano@aosp.bo.it
00390512144816
Rita Ortolano, MD
principal investigator · IRCCS Azienda Ospedaliero-Universitaria di Bologna

Oversight

Data monitoring committee
No
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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