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CompletedNCT05841407Updated Apr 9, 2024

Beetroot Supplementation and High Intensity Exercise

An interventional study of High nitrate isotonic drink and No nitrate isotonic drink in Exercise Performance, sponsored by Nanyang Technological University. Completed at 1 site in Singapore. Open to participants aged 21 Years to 40 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2024-04-09.

Sponsored by Nanyang Technological University · Not applicable, Interventional, and Other

Phase
Not applicable
Study type
Interventional
Enrollment
24
Allocation
Randomized
Ages
21 Years to 40 Years
Sex
All
01

Study summary

Ingested beetroot contains inorganic nitrate which is later converted to nitric oxide (NO) in the bloodstream. NO is an important signaling molecule with several physiological functions in the body including acting as a vasodilator, expanding arteries and improving blood flow. Beetroot juice contain inorganic nitrate and consumption of beetroot has been shown to improve skeletal muscle contractility and metabolism enhancing high-intensity exercise performance. Isotonic sports drinks contain carbohydrates and sodium, which also improve exercise performance by providing exogenous glucose for energy to the working muscle and central nervous system and enabling faster absorption of fluid into the bloodstream. The ingestion of exogenous nitrate and isotonic-carbohydrate drink are recognized strategies for promoting exercise performance but whether they can be effectively combined has not been shown. This study will compare the salivary nitrite response, muscle oxygenation and time-trial performance after ingestion of two isotonic drinks with high in nitrate or with no nitrate.

Read the detailed description

Ingested beetroot contains inorganic nitrate (NO3-) which is later converted to nitric oxide (NO) in the bloodstream. NO is an important signaling molecule with several physiological functions in the body including acting as a vasodilator, expanding arteries and improving blood flow. These functions mean that beetroot juice has become of strong interest in the field of exercise and is a recommended supplement for athletes to improve performance in recent consensus statements by the International Olympic Committee on dietary supplements. Importantly, the consumption of beetroot has been shown to improve skeletal muscle contractility and metabolism, hence, enhancing high-intensity exercise performance . Isotonic sports drinks contain carbohydrates and sodium, which also improve exercise performance by providing exogenous glucose for energy to the working muscle and central nervous system and enabling faster absorption of fluid into the bloodstream. The ingestion of exogenous nitrate and isotonic-carbohydrate drink are recognized strategies for promoting exercise performance but whether they can be effectively combined has not been shown. This study will compare the salivary nitrite response, muscle oxygenation and time-trial performance after ingestion of two drinks either high in nitrate or with no nitrate. Participants will ingest the two drinks in a randomised, crossover double-blind trial. One hour after drink ingestion they will undertake high intensity intermittent exercise for 30 minutes followed by a 10 km time trial on a cycle ergometer. Time trial will be compared between trials as the main outcome. It is hypothesised that the drink high in nitrate will lead to faster time trial completion.

02

Conditions studied

  • Exercise Performance
03

In context

Lead sponsor

Nanyang Technological University is the lead sponsor of 48 studies on the registry; 14 are open to participants now.

Counted across the registry records on this site, refreshed daily.

04

Who can participate

Ages eligible
21 Years to 40 Years
Sexes eligible
All
Accepts healthy volunteers
Yes

Inclusion criteria

  • Age: 21 - 40 years
  • Healthy and injury-free (e.g., no cardiovascular disease, diabetes mellitus, orthopaedic impairment that interferes with moderate-to-vigorous exercise)
  • Blood pressure \< 130/90 mmHg
  • Physically active: Engaging in a minimum of 150 minutes of moderate-intensity exercise or 75 minutes of vigorous-intensity physical activity each week.
  • Recreational cyclist (Minimum 1 hour cycling per week)
  • Body mass index: 18.5-25 kg/m2
  • Non-smoker
  • Asian ethnicity by self identification

Exclusion criteria

Exclusion Criteria:

  • Any allergies to beetroot or isotonic drink.
  • Asthma.
  • Any report of personal health issues that may compromise the individual's ability to undertake exercise safely.
  • Any recent Covid vaccination (within last 2 weeks).
05

Study design

Phase
Not applicable
Primary purpose
Other
Allocation
Randomized
Intervention model
Crossover assignment
Masking
Double (Participant, Investigator)
Enrollment
24 participants (actual)

Study arms

  • Experimental
    High nitrate

    High nitrate isotonic drink

    Dietary Supplement: High nitrate isotonic drink

  • Active comparator
    No nitrate

    No nitrate isotonic drink

    Dietary Supplement: No nitrate isotonic drink

Interventions

  • Dietary supplementHigh nitrate isotonic drink

    High nitrate (12.9 mmol) drink with carbohydrate and minerals

  • Dietary supplementNo nitrate isotonic drink

    Carbohydrate and mineral isotonic drink

06

What researchers measure

Primary outcomes

  1. 10 km cycling time trial (endurance) performance

    Time trial

    Time frame: 1 hour after drink ingestion the time taken to complete 10 km on a stationary cycle ergometer will be assessed. Time to complete this is the primary outcome measure .

Secondary outcomes

  1. Salivary nitrate

    Salivary nitrate concentration determined from strips

    Time frame: Four samples over 2 hours

  2. Near infra-red spectroscopy (NIRS)

    Tissue saturation index

    Time frame: During cycling ~1 hour

07

Study locations

1 site
  • Human Bioenergetics Laboratory, National Institute of Education, Nanyang Technological University
    Singapore, 637616, Singapore
08

References and documents

Individual participant data

Plan to share: Yes — All published data will be placed into the Data Repository of Nanyang Technological University National Institute of Education for public access upon publication.

Supporting information: Analytic code

No publications or documents are linked to this record.

09

Updates

Tracking since Sep 25, 2026
No changes since tracking began. The registry record was last updated on Apr 9, 2024, before this site started recording changes on Sep 25, 2026. Its history is on ClinicalTrials.gov ↗
10

Registry details

Key details

Study ID
NCT05841407
Lead sponsor
Nanyang Technological University
Responsible party
Stephen Burns (Associate Professor, Nanyang Technological University) — Principal investigator
First posted
May 3, 2023
Start date
Mar 15, 2023
Primary completion
Mar 14, 2024
Completion
Mar 14, 2024
Last update
Apr 9, 2024

Study contacts

Stephen Burns, PhD
principal investigator · Nanyang Technological University

Oversight

FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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This study is completed, as verified in Apr 2024. You cannot join it, but the record below documents what was studied.

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