An interventional study of High nitrate isotonic drink and No nitrate isotonic drink in Exercise Performance, sponsored by Nanyang Technological University. Completed at 1 site in Singapore. Open to participants aged 21 Years to 40 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2024-04-09.
Sponsored by Nanyang Technological University · Not applicable, Interventional, and Other
Ingested beetroot contains inorganic nitrate which is later converted to nitric oxide (NO) in the bloodstream. NO is an important signaling molecule with several physiological functions in the body including acting as a vasodilator, expanding arteries and improving blood flow. Beetroot juice contain inorganic nitrate and consumption of beetroot has been shown to improve skeletal muscle contractility and metabolism enhancing high-intensity exercise performance. Isotonic sports drinks contain carbohydrates and sodium, which also improve exercise performance by providing exogenous glucose for energy to the working muscle and central nervous system and enabling faster absorption of fluid into the bloodstream. The ingestion of exogenous nitrate and isotonic-carbohydrate drink are recognized strategies for promoting exercise performance but whether they can be effectively combined has not been shown. This study will compare the salivary nitrite response, muscle oxygenation and time-trial performance after ingestion of two isotonic drinks with high in nitrate or with no nitrate.
Ingested beetroot contains inorganic nitrate (NO3-) which is later converted to nitric oxide (NO) in the bloodstream. NO is an important signaling molecule with several physiological functions in the body including acting as a vasodilator, expanding arteries and improving blood flow. These functions mean that beetroot juice has become of strong interest in the field of exercise and is a recommended supplement for athletes to improve performance in recent consensus statements by the International Olympic Committee on dietary supplements. Importantly, the consumption of beetroot has been shown to improve skeletal muscle contractility and metabolism, hence, enhancing high-intensity exercise performance . Isotonic sports drinks contain carbohydrates and sodium, which also improve exercise performance by providing exogenous glucose for energy to the working muscle and central nervous system and enabling faster absorption of fluid into the bloodstream. The ingestion of exogenous nitrate and isotonic-carbohydrate drink are recognized strategies for promoting exercise performance but whether they can be effectively combined has not been shown. This study will compare the salivary nitrite response, muscle oxygenation and time-trial performance after ingestion of two drinks either high in nitrate or with no nitrate. Participants will ingest the two drinks in a randomised, crossover double-blind trial. One hour after drink ingestion they will undertake high intensity intermittent exercise for 30 minutes followed by a 10 km time trial on a cycle ergometer. Time trial will be compared between trials as the main outcome. It is hypothesised that the drink high in nitrate will lead to faster time trial completion.
Nanyang Technological University is the lead sponsor of 48 studies on the registry; 14 are open to participants now.
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Exclusion Criteria:
High nitrate isotonic drink
Dietary Supplement: High nitrate isotonic drink
No nitrate isotonic drink
Dietary Supplement: No nitrate isotonic drink
High nitrate (12.9 mmol) drink with carbohydrate and minerals
Carbohydrate and mineral isotonic drink
10 km cycling time trial (endurance) performance
Time trial
Time frame: 1 hour after drink ingestion the time taken to complete 10 km on a stationary cycle ergometer will be assessed. Time to complete this is the primary outcome measure .
Salivary nitrate
Salivary nitrate concentration determined from strips
Time frame: Four samples over 2 hours
Near infra-red spectroscopy (NIRS)
Tissue saturation index
Time frame: During cycling ~1 hour
Plan to share: Yes — All published data will be placed into the Data Repository of Nanyang Technological University National Institute of Education for public access upon publication.
Supporting information: Analytic code
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This study is completed, as verified in Apr 2024. You cannot join it, but the record below documents what was studied.
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Nanyang Technological University