An interventional study of Biogaia L. reuteri Prodentis® lozenges and Hersheyland Ice Breakers Mints sugar free lozenges in White Spot Lesion, sponsored by Jordan University of Science and Technology. Completed at 1 site in Jordan. Open to participants aged 17 Years to 33 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2026-04-09.
Sponsored by Jordan University of Science and Technology · Not applicable, Interventional, and Prevention
The goal of this randomized clinical trial was to investigate the effect of probiotic lozenges on the development of white spot lesions (WSLs) compared to a positive control, and no treatment.
Background: Recently, the use of probiotics has emerged as a treatment for prevention of oral cavity diseases including periodontal problems, and initial caries. The objectives of this prospective randomized clinical trial was to investigate the effect of probiotic lozenges on the development of white spot lesions (WSLs) compared to a positive control, and no treatment. Methods: Quantitative light induced fluorescence (QLF) images were taken for three randomly allocated groups (intervention group, n=23; positive control, n=23; negative control, n=24) of patients undergoing orthodontic treatment in three separate occasions (T0: before the start of study; T1: at 6 weeks later; T2: at 3 months after the commencement of the study). Subjects in the intervention group were instructed to take one probiotic lozenge containing two strains of Lactobacillus reuteri twice daily. Sugar-free lozenges were used in the positive control group and no lozenges for the negative control group. The QLF images were analyzed using a dedicated software in order to quantitatively analyze each tooth. The primary outcomes measured were the number of newly developed lesions in each group, lesion area and amount of mineral loss (ΔF). Means and standard deviations were calculated using the Statistical Package for the Social Science (SPSS version 28, Chicago, IL, USA). Split plot ANOVA (SPANOVA) test for repeated measures was used to check differences within and between the three groups regarding lesion area and ΔF. To check which group is different from the others, Tukey's test was used.
Jordan University of Science and Technology is the lead sponsor of 88 studies on the registry; 7 are open to participants now.
Counted across the registry records on this site, refreshed daily.
Exclusion Criteria:
Participants received treatment with Biogaia L. reuteri Prodentis® lozenges
Dietary Supplement: Biogaia L. reuteri Prodentis® lozenges
Participants received Hersheyland Ice Breakers Mints sugar free lozenges
Dietary Supplement: Hersheyland Ice Breakers Mints sugar free lozenges
Participants received no lozenges or supplements
Participants were instructed to use the lozenges twice daily after brushing their teeth; once in the morning and once in the evening
Hersheyland Ice Breakers Mints sugar free lozenges
Incidence of white spot lesions (WSL)
Number of newly developed white spot lesions
Time frame: 3 months
Lesion area
Surface area of the WSL (in pixels)
Time frame: 3 months
ΔF%
Average lesion fluorescence loss (ΔF%) reflecting mineral loss in percent.
Time frame: 3 months
ΔFMax (%)
Deepest point in the lesion
Time frame: 3 months
ΔR30 (%)
Amount of plaque present
Time frame: 3 months
Plan to share: No — Data will be available upon request
This study is completed, as verified in Apr 2026. You cannot join it, but the record below documents what was studied.
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Jordan University of Science and Technology