CClinicalTrials.gg
CompletedNCT04833582Updated Apr 3, 2026

A Study of Azenosertib (ZN-c3) in Combination With Gemcitabine in Subjects With Osteosarcoma

A Phase 1 interventional study of Azenosertib and Gemcitabine in Osteosarcoma, sponsored by K-Group, Beta, Inc., a wholly owned subsidiary of Zentalis Pharmaceuticals, Inc. Completed at 17 sites in 2 countries. Open to participants aged 12 Years and older. Per ClinicalTrials.gov, last updated 2026-04-03.

Sponsored by K-Group, Beta, Inc., a wholly owned subsidiary of Zentalis Pharmaceuticals, Inc · Phase 1, Interventional, and Treatment

Phase
Phase 1
Study type
Interventional
Enrollment
31
Allocation
Not applicable
Ages
12 Years and older
Sex
All
01

Study summary

This is a phase 1/2 study of azenosertib (ZN-c3) in combination with gemcitabine in adult and pediatric subjects with relapsed or refractory osteosarcoma.

Read the detailed description

This is a phase 1/2 dose escalation and dose expansion study, evaluating the clinical activity and safety, pharmacodynamics, and pharmacokinetics of azenosertib (ZN-c3) in combination with gemcitabine in relapsed or refractory osteosarcoma.

02

Conditions studied

  • Osteosarcoma

Browse trials for

03

Who can participate

Ages eligible
12 Years and older
Sexes eligible
All
Accepts healthy volunteers
No

Inclusion criteria

  • Age ≥ 12 years at the time of informed consent
  • Bodyweight ≥ 40 kg
  • Histologically documented relapsed or metastatic osteosarcoma.
  • Must have measurable disease according to RECIST Guideline version 1.1 criteria.
  • Adequate hematologic and organ function.
  • Female subjects of childbearing potential and male subjects must agree to use an effective method of contraception per institutional standard prior to the first dose and for 6 months after study treatment discontinuation.
  • Willingness and ability to comply with scheduled visits, treatment plan, laboratory tests, and other study procedures.

Exclusion criteria

Exclusion Criteria:

  • Unresolved toxicity of Grade >1 attributed to prior therapies (excluding: Grade ≤2 neuropathy, alopecia, or skin pigmentation)
  • Prior therapy with a WEE1 inhibitor
  • A serious illness or medical condition(s).
  • Pregnant or lactating females. Females of childbearing potential with a positive serum pregnancy test \<14 days to Day 1.
  • Subjects with active (uncontrolled, metastatic) second malignancies or requiring therapy.
  • 12-lead ECG demonstrating a corrected QT interval using Fridericia's formula (QTcF) of >470 ms, except for subjects with atrioventricular pacemakers or other conditions (e.g., right bundle branch block) that render the QT measurement invalid.
  • History or current evidence of congenital or family history of long QT syndrome or Torsades de Pointes (TdP).
  • Taking medications with a known risk of TdP.
  • Administration of strong and moderate CYP3A4 inhibitors/inducers and strong and moderate P-gp inhibitors.
04

Study design

Phase
Phase 1
Primary purpose
Treatment
Allocation
Not applicable
Intervention model
Sequential assignment
Masking
None (open label)
Enrollment
31 participants (actual)

Study arms

  • Experimental
    Azenosertib in combination with Gemcitabine

    Azenosertib (ZN-c3) in combination with Gemcitabine

    Drug: Azenosertib · Drug: Gemcitabine

Interventions

  • DrugAzenosertib

    Azenosertib is an investigational drug.

    Also known as: ZN-c3

  • DrugGemcitabine

    Gemcitabine is an approved drug

    Also known as: Gemzar

05

What researchers measure

Primary outcomes

  1. Incidence of dose-limiting toxicities (DLT) in DLT evaluable subjects and the incidence and severity of adverse events.

    Time frame: Through Cycle 1 (21 days) Phase 1

  2. Event-free survival (EFS) at 18 weeks per RECIST (Response Evaluation Criteria in Solid Tumors) Guideline version 1.1.

    EFS at 18 weeks is defined as time from study enrollment until date of disease progression, or detection of disease at a previously uninvolved site, or date of death of the subjects at 18 weeks.

    Time frame: During phase 2, at 18 weeks

Secondary outcomes

  1. Event-free survival (EFS) per RECIST Guideline version 1.1.

    EFS is defined as time from study enrollment until date of last contact, date of disease progression, or detection of disease at a previously uninvolved site, or date of death.

    Time frame: At 12 months

  2. Median overall survival (OS) and OS at 12 months per RECIST Guideline version 1.1.

    OS is defined as the time from date of first dosing until the date of death.

    Time frame: At 12 months

  3. The frequency and severity of adverse events (AEs) and laboratory abnormalities per the National Cancer Institute Common Terminology (NCI CTCAE) version 5.0.lities.

    Time frame: Through completion, approximately 42 months

  4. Plasma pharmacokinetics (PK) maximum concentration (Cmax).

    Time frame: Through completion, approximately 42 months

  5. Plasma PK time to maximum concentration (Tmax).

    Time frame: Through completion, approximately 42 months

  6. Area under the plasma concentration versus timepoint curve (AUC last).

    Time frame: Through completion, approximately 42 months

  7. Terminal half-life of the plasma PK concentration.

    Time frame: Through completion, approximately 42 months

06

Study locations

17 sites
  • Site 0106
    Los Angeles, California 90095, United States
  • Site 0124
    Oakland, California 94609, United States
  • Site 0195
    Santa Monica, California 90403, United States
  • University of Florida College of Medicine
    Gainesville, Florida 32610, United States
  • Site 0105
    New York, New York 10065, United States
  • Site 0107
    Cincinnati, Ohio 45229, United States
  • Site 0123
    Portland, Oregon 97239, United States
  • Site 0193
    Memphis, Tennessee 38105, United States
  • Site 0197
    Nashville, Tennessee 37332, United States
  • Site 0103
    Houston, Texas 77030, United States
  • Site 0188
    Richmond, Virginia 23298, United States
  • Site 0122
    Seattle, Washington 98195, United States
  • Site 3604
    Bordeaux, 33000, France
  • Site 3601
    Lyon, 69008, France
  • Site 3602
    Marseille, 13385, France
  • Site 3606
    Paris, 75248, France
  • Site 3605
    Toulouse, 31100, France
07

Registry details

Key details

Study ID
NCT04833582
Lead sponsor
K-Group, Beta, Inc., a wholly owned subsidiary of Zentalis Pharmaceuticals, Inc
Responsible party
Sponsor
First posted
Apr 6, 2021
Start date
Aug 1, 2021
Primary completion
Aug 30, 2023
Completion
Mar 30, 2024
Last update
Apr 3, 2026

Oversight

Data monitoring committee
Yes
FDA-regulated drug
Yes
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

Not currently enrolling

This study is completed, as verified in Mar 2026. You cannot join it, but the record below documents what was studied.

Follow this study

Get an email when the registry record changes — status, dates, results — or when someone posts here.

Sign in to follow

Discussion

Questions and observations about this study, from anyone following it. Not medical advice, and not a channel to the study team — their contact details are on the registry record.

Sign in to join the discussion. Reading takes no account; posting does. You choose a display name, and a pseudonym is the default.

Nothing here yet. If you are running this trial, taking part in it, or weighing whether to, this is the place to say so.

Start the discussion