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CompletedNCT04829630RESIST-3Updated Mar 9, 2023

Immunity Persistence After Abridged Intradermal Rabies PEP

An interventional study of rabies vaccine in Rabies, sponsored by Institut Pasteur. Completed at 2 sites in 2 countries. Per ClinicalTrials.gov, last updated 2023-03-09.

Sponsored by Institut Pasteur · Not applicable, Interventional, and Other

Phase
Not applicable
Study type
Interventional
Enrollment
180
Allocation
Not applicable
Sex
All
01

Study summary

After exposure, rabies can be prevented in almost 100% of cases by the administration of sufficient and timely post-exposure prophylaxis (PEP). PEP is based on wound cleansing, antisepsis, administration of rabies vaccine as well as rabies immunoglobulin, if reviewed. However, anti-rabies PEP remains too often out of financial and / or geographic access, especially for poor and / or rural populations in endemic countries who remain the most exposed to the risk of contracting rabies. Two major studies planned in Cambodia between 2014 and 2018 - the RESIST 0/1 clinical - epidemiological study and the RESIST-2 study on the antibody response to the vaccine - provided the basis that allowed a change in international recommendations on PPE. Since April 2018, the new "IPC protocol" of three sessions of reduced double doses (0.1 mL x 2) administered intradermally (ID) over one week has replaced the already very effective "TRC protocol" of four sessions over one month which was the reference dose-sparing protocol for endemic countries until 2018. It remains to be determined whether the IPC protocol (3 sessions / 1 week) confers long-term immunity equivalent to that obtained after a TRC ID protocol (4 sessions / 1 month). This question is of importance to public health decision-makers and clinical teams in endemic countries who would hesitate to switch to the abbreviated IPC protocol.

Read the detailed description

This is a multicenter, randomized, non-inferior clinical trial with samples for examinations and vaccination booster.

To achieve this study, persons who previously received PEP and of any age (including children), gender or medical condition (including pregnant women) and whichever the status of the dog which bit at least 2, 5 or 10 years ago will be contacted and invited to participate to the study.

This study will take place in two centers :

  • The rabies prevention center at Institut Pasteur du Cambodge
  • The rabies prevention center at Institut Pasteur de Madagascar. The study will be coordinated by Institut Pasteur in Paris which will subsequently conduct the analysis in collaboration with researchers at Institut Pasteur du Cambodge and Institut Pasteur de Madagascar.

This study will take place in two visits :

  • the first visit (day 0) : after receiving consent from the participants, the member of the investigating team will perform the blood sample, followed by a single session of two intradermal doses of 0.1 mL. The investigators will also carry out measurements and complete the questionnaire with the participant.
  • the second visit (day 7) : a follow-up visit will be performed by a member of the investigating team who will perform a second blood sample to all the participants to the study. The investigator will complete a second paper CRF with the participants to document any unwanted effects which may have occurred during the seven days between the inclusion visit and the follow-up visit.
02

Conditions studied

  • Rabies

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Keywords

  • rabies
  • vaccine
  • Post-exposure prophylaxis
  • Persistent immunity
  • Short protocol
03

Who can participate

Ages eligible
Child (0–17), Adult (18–64), Older adult (65+)
Sexes eligible
All
Accepts healthy volunteers
Yes

Inclusion criteria

  • person Identified in IP Cambodia or IP Madagascar records for having received ID PEP by 4 sessions/1 month or 3 sessions/1 week at least two, five or 10 years earlier, with no rabies boosting since that time;
  • person Who received information adapter to his/her age and who signed the consent form (or his/her legal tutors)

Exclusion criteria

Exclusion Criteria:

  • person who received Rabies vaccine booster since the earlier PEP
  • person with allergy or other severe unwanted effect at the time of the earlier PEP
04

Study design

Phase
Not applicable
Primary purpose
Other
Allocation
Not applicable
Intervention model
Single group
Masking
None (open label)
Enrollment
180 participants (actual)

Study arms

  • Other
    subjects who received three vs. four ID PEP at least two, five or 10 years earlier

    * 30 subjects who received three ID PEP at least two years earlier in Cambodia * 30 subjects who received three ID PEP at least five years earlier in Cambodia * 30 subjects who received three ID PEP at least 10 years earlier in Cambodia * 30 subjects who received four ID PEP at least two years earlier in Madagascar * 30 subjects who received four ID PEP at least five years earlier in Madagascar * 30 subjects who received four ID PEP at least 10 years earlier in Madagascar

    Biological: rabies vaccine

Interventions

  • Biologicalrabies vaccine

    * day 0 : questionnaire, blood sampling and vaccine booster (a single session of two intradermal doses of 0.1 mL) * day 7 : questionnaire and blood sampling

05

What researchers measure

Primary outcomes

  1. Antibody titers measured (FAVN, RFFIT) and cellular immunity markers one week after a single session of ID rabies vaccine boosting in the two groups

    Absence of statistically significant difference between antibody titers Fluorescent Antibody Virus Neutralization test (FAVN) and Rapid Fluorescent Foci Inhibition Test (RFFIT) and cellular immunity markers one week after a single session of ID rabies vaccine boosting in persons who have received a 3 sessions/1 week vs. 4 sessions/ 1 month ID PEP at least 2, 5 or 10 years earlier.

    Time frame: 1 year

Secondary outcomes

  1. Antibody titers measured by FAVN and RFFIT and immune response before a single session of ID rabies vaccine boosting in the two groups

    Equivalence of antibody titers and immune response before a single session of ID rabies vaccine boosting in persons who have received a 3 sessions/1 week vs. 4 sessions/ 1 month ID PEP at least 2, 5 or 10 years earlier.

    Time frame: 1 year

  2. Antibody titers measured by FAVN and RFFIT in all subjects for comparison

    Equivalence of antibody titers measured using FAVN compared to the RFFIT used as reference.

    Time frame: 1 year

06

Study locations

2 sites
  • Institut Pasteur du Cambodge
    Phnom Penh, Cambodia
  • Institut Pasteur de Madagascar
    Antananarivo, BP 1274, Madagascar
07

Registry details

Key details

Study ID
NCT04829630
Lead sponsor
Institut Pasteur
Collaborators
Institut Pasteur du Cambodge, Institut Pasteur de Madagascar
Responsible party
Sponsor
First posted
Apr 2, 2021
Start date
Oct 20, 2021
Primary completion
Feb 1, 2023
Completion
Feb 1, 2023
Last update
Mar 9, 2023

Study contacts

Perrine Parize, MD
study director · Institut Pasteur

Oversight

Data monitoring committee
No
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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