CClinicalTrials.gg
CompletedNCT04543149Updated Jan 12, 2021

Immune Cells in Patients With Granulomatous Mastitis

An observational study in Granulomatous Mastitis, sponsored by Istanbul Training and Research Hospital. Completed at 1 site in Turkey. Open to female participants aged 18 Years to 80 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2021-01-12.

Sponsored by Istanbul Training and Research Hospital · Observational

Study type
Observational
Model
Case-control
Time perspective
Prospective
Enrollment
35
Ages
18 Years to 80 Years
Sex
Female
01

Study summary

Granulomatous mastitis is a rare, chronic, benign inflammatory disease of the breast that plays a role in many varying etiologies, including infectious and non-infectious causes. Etiological reasons were examined in various studies, but neither etiology nor definite criteria for diagnosis were found. Our aim in this study is to examine the role of immunophenotyping in differentiating the etiology of granulomatous mastitis and using it as a prognostic marker.

Read the detailed description

Granulomatous mastitis is a rare, chronic, benign inflammatory disease of the breast that plays a role in many varying aetiology, including infectious and non-infectious causes. This disease can clinically and radiographically mimic breast cancer, which can pose a diagnostic challenge as well as concern during evaluation. A nonspecific inflammatory response, 'granuloma' is defined as necrosis or infiltration of other inflammatory leukocytes, with or without mononuclear phagocyte collection. It is thought to be caused by infectious agents or foreign substances that activate the immune system, leading to granuloma formation. Some of the known inflammatory etiologies of granulomatous mastitis are autoimmune diseases such as tuberculosis (most commonly caused by Mycobacterium tuberculosis), sarcoidosis, fungal infection and granulomatosis with polyangiitis and giant cell arteritis. These diseases cannot be distinguished clinically, pathologically, and radiographically from idiopathic granulomatous mastitis.In this study, we aimed to investigate lymphocyte subtypes in patients with granulomatous mastitis and to evaluate the differences in cell populations and its usability as a predictive marker in diagnosis.

02

Conditions studied

  • Granulomatous Mastitis

Keywords

  • Mastitis
  • immunophenotyping
  • Flow-cytometry
03

Who can participate

Ages eligible
18 Years to 80 Years
Sexes eligible
Female
Accepts healthy volunteers
Yes
Sampling method
Non-probability sample

Study population

In case group patients with granulomatous mastitis will be included to the study. During diagnasis if the patient is diagnosed with tuberculosis, she will be excluded from the study.

Also healty volunteers who don't have breast lesions are control group.

Inclusion criteria

  • Patients with granulomatous mastitis

Exclusion criteria

Exclusion Criteria:

  • Cancer Patients
  • Patients with known immunodeficiency
  • Pregnancy
  • Patient diagnosed with tuberculosis
04

Study design

Observational model
Case-control
Time perspective
Prospective
Enrollment
35 participants (actual)
Patient registry
No

Groups and cohorts

  • Case

    Patients with idiopathic granulomatous mastitis

    Diagnostic Test: Flow cytometric immunophenotyping

  • Control

    Healthy volunteers

    Diagnostic Test: Flow cytometric immunophenotyping

Interventions

  • Diagnostic testFlow cytometric immunophenotyping

    To evaluate T cell subsets and monocyte

05

What researchers measure

Primary outcomes

  1. To evaluate T cell subsets and monocyte ratio

    CD3 (+) lymphocytes, CD4 (+) lymphocytes, CD 8 (+) lymphocytes, CD 3(-)/CD16 (+) NK cell ratio, CD14 (+) lymphocytes, and HLA-DR positivity on CD14 (+) cells

    Time frame: 3 months

06

Study locations

1 site
  • Istanbul Training and Research Hospital
    Istanbul, 34371, Turkey
07

References and documents

Publications

  • Barreto DS, Sedgwick EL, Nagi CS, Benveniste AP. Granulomatous mastitis: etiology, imaging, pathology, treatment, and clinical findings. Breast Cancer Res Treat. 2018 Oct;171(3):527-534. doi: 10.1007/s10549-018-4870-3. Epub 2018 Jul 3. PubMed 29971624 ↗
  • Skandarajah A, Marley L. Idiopathic granulomatous mastitis: a medical or surgical disease of the breast? ANZ J Surg. 2015 Dec;85(12):979-82. doi: 10.1111/ans.12929. Epub 2014 Nov 26. PubMed 25424519 ↗

Individual participant data

Plan to share: Undecided

08

Registry details

Key details

Study ID
NCT04543149
Lead sponsor
Istanbul Training and Research Hospital
Responsible party
Ufuk Oguz Idiz (Assoc. Prof. MD, Istanbul Training and Research Hospital) — Principal investigator
First posted
Sep 9, 2020
Start date
Aug 1, 2020
Primary completion
Oct 1, 2020
Completion
Oct 10, 2020
Last update
Jan 12, 2021

Study contacts

Ufuk Oguz Idiz, Assoc.Prof.
principal investigator · Istanbul Training and Research Hospital

Oversight

Data monitoring committee
Yes
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

Not currently enrolling

This study is completed, as verified in Jan 2021. You cannot join it, but the record below documents what was studied.

Follow this study

Get an email when the registry record changes — status, dates, results — or when someone posts here.

Sign in to follow

Discussion

Questions and observations about this study, from anyone following it. Not medical advice, and not a channel to the study team — their contact details are on the registry record.

Sign in to join the discussion. Reading takes no account; posting does. You choose a display name, and a pseudonym is the default.

Nothing here yet. If you are running this trial, taking part in it, or weighing whether to, this is the place to say so.

Start the discussion