An interventional study of Milk Protein Concentrate in Muscle Protein Synthesis and Protein Metabolism, sponsored by University of Illinois at Urbana-Champaign. Completed at 1 site in United States. Open to male participants aged 18 Years to 35 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2019-10-10.
Sponsored by University of Illinois at Urbana-Champaign · Not applicable, Interventional, and Basic science
The anabolic action of 'fast' whey protein on the regulation of postprandial muscle protein synthesis has been established to be short-lived in healthy young adults. Our aim was assess the time course of anabolic signaling events and stimulation of muscle protein synthesis rates (MPS) after ingestion of a food source that represents a more typical meal-induced pattern of aminoacidemia, namely milk protein concentrate, in healthy young males.
University of Illinois at Urbana-Champaign is the lead sponsor of 188 studies on the registry; 29 are open to participants now.
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Seven young men (age: 22±1 y) will undergo repeated blood and biopsy sampling during primed continuous L-\[ring-2H5\]phenylalanine and L-\[1-13C\]leucine tracer infusions, and ingested 38 g of L-\[1-13C\]phenylalanine- and L-\[1-13C\]leucine-labeled milk protein concentrate
Dietary Supplement: Milk Protein Concentrate
The macronutrient composition and energy of the milk protein beverage provided to participants is 38 g protein (3.46 g leucine), 4.17 g carbohydrate, and 1.4 g fat. The milk protein met all chemical and bacteriologic specifications for human consumption. The L-\[1-13C\]phenylalanine and L-\[1-13C\]leucine enrichments in the milk protein concentrate averaged 38.3 and 10.8 mole percent excess (MPE), respectively.
Fractional synthesis rates of myofibrillar protein
Myofibrillar protein synthesis rates will be assessed during the postabsorptive period for 3 hours, and every 1-2 hours during the 5 hours after protein ingestion. This will allow us assess the timecourse of muscle protein synthesis after eating.
Time frame: 8 hours
Phosphorylation of muscle anabolic signaling
Phosphorylation of anabolic signaling pathways (mTORC1, AKT, and P70S6K) involved in the stimulation of myofibrillar protein synthesis rates will be assessed during the postabsorptive period for 3 hours, and every 1-2 hours during the 5 hours after protein ingestion.
Time frame: 8 hours
Exogenous rate of phenylalanine appearance
Dietary derived phenylalanine availability will be measured during the 5 h postprandial phase to determine its relationship with the fractional synthesis rates of myofibrillar protein and phosphorylation of muscle anabolic signaling
Time frame: 5 hours
Plan to share: No
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University of Illinois at Urbana-Champaign