Biomarker Study of PDR001 in Combination With MCS110 in Gastric Cancer

\<Background>

1. Current status of treatment options in advanced gastric cancer.

2. Current status of immunotherapy development in gastric cancer

   • Harnessing immune system to fight against cancer cells is one of highlighting strategy in new drug development in almost all kinds of solid tumor including gastric cancer.
   • In PD L1expressing gastric cancer, the anti-PD1 antibody monotherapy (pembrolizumab) shows the antitumor efficacy. (Bang YJ et al. 2015)
   • Anti-PDL1 antibody (Avelumab) used in the first-line maintenance setting or 2nd-line setting of biomarker-unselected gastric cancer also shows promising antitumor efficacy (Chung HC et al. ECCO/ESMO 2015, Oh DY et al. ASCO GI 2016)
   • There are many clinical trials using immune-oncology (IO) agents are ongoing in 1st-line setting, 2nd-line setting, 3rd-line setting and refractory setting. Several strategies are being used, that is, IO monotherapy in biomarker-selected population, IO+IO combination, IO+cytotoxic chemotherapy , and IO+other targeted agents to improve the efficacy of harnessing immune system.
   • Still, we don't know the exact biomarker for these IO agents in gastric cancer. Not all patients get the benefit with these agents.

3. The importance of tumor microenvironment

   • From the immunotherapy point of view, the tumor microenvironment (TME) should be considered for the successful immunotherapy.
   • In TME, many components working for antitumor immunity and intratumoral immunosuppression exist. (Pitt JM et al. 2016)
   • The most crucial and direct control of tumor cells is performed by CD4+ T helper cell and CD8+ cytotoxic T lymphocyte (CTL). Th1 response, characterized by T cell production of IFN-ɣ, TNF-α, IL-2 are considered to be the essential subset for tumor rejection. However, Th1 response also contributes to tumor escape via IFN-ɣ-mediated expression of the inhibitory checkpoint molecules PDL1 or via the selection of resistant clones through tumor immunoediting.
   • Long-term exposure to tumor antigen induces Th1 cells and other T cells that lack the typical poly-functional phenotype and that express inhibitory receptors such as PDL1. LAG-3, and TIM-3.
   • Other subsets of CD4+T cells inhibit antitumor immune responses. CD4+Tregs is the example. Tregs inhibit the antitumor activity of CTLs and NK cells either directly, or indirectly via APCs. In addition to Tregs, Th2 cells can also block Tcell-induced tumor rejection.
   • TME can also directly impair intratumoral T cell proliferation, acting as another mechanism for tumor evasion of immune surveillance. IDO production within the TME is the example. IDO produced by myeloid cells and cancer cells catabolizes tryptophan to generate kynurenine, which together promotes the conversion of naïve T cells to Tregs,inhibition of Treg reprogramming to potentially anti-tumoral Th cells, and increases MDSC functions through upregulation of IL-6 expression.

4. The role of polarized macrophage in TME

   • Macrophages are one of the major components of the TME, are recruited by chemokines such as M-CSF and chemokine C-C motif ligand 2, and are produced mainly be tumor cells (Wiktor-Jedrzejczak W, et al. 1996)
   • Macrophages are dynamic cells that can express different functional programs in response to microenvironmental signals (Mantovani A, et al. 2004)
   • Bacterial stimuli, IFN-ɣ and GM-CSF promote a proinflammatory M1-polarized phenotype, whereas M-CSF, IL-4, and IL-13 favor the generation of folate receptor b positive, IL-10 producing, immunosuppressive, M2-polarized macrophages. (Murray PJ, et al. 2011). M1 macrophages also amplify TH1 responses, providing a positive feedback loop in the antitumor response.
   • Macrophages that infiltrate tumor tissues, also termed tumor-associated macrophages (TAMs), can be driven by tumor-derived and T-cell derived cytokines to acquire such a polarized phenotype, and have a key role in subversion of adaptive immunity and in inflammatory circuits that promote tumor growth and progression. (Mantovani A, et al. 2002)
   • Fully polarized M1 and M2 macrophages are the extremes of a continuum of functional states; in contrast to this binary M1/M2 definition, there are several other distinct populations that share features of both types.( Ojalvo LS, et al. 2009)
   • The M2-polarized macrophages promote tumor progression and metastasis by activating circuits that regulate tumor growth, adaptive immunity, stroma formation, and angiogenesis, and can be used as prognostic indicators. ( Balkwill F, et al. 2001)

5. The role of polarized macrophage in gastric cancer

   • In gastric cancer, infiltration of polarized TAMs is an independent prognostic factor. (Zhang, et al. 2015)
   • The number of peritoneal macrophages with the M2 phenotype (CD68+CD163+ or CD68+CD204+) was significantly higher in gastric cancer patients with peritoneal dissemination than in those without peritoneal dissemination. (Yamaguchi et al. 2015)
   • Higher expression of M2-related messenger RNA (IL-10, VEGF-A, VEGF-C, MMP-1, amphiregulin), lower expression of M1-related messenger RNA (TNF-am CD80, CD86, IL-12p40) were confirmed in the TAM of gastric cancer.
   • Interestingly, macrophage co-culture with gastric cancer cells converted M1 phenotype into M2 phenotype. Moreover, the coexistence of MKN45 cells with M2 macrophages resulted in cancer cell proliferation and an acceleration of tumor growth in the xenograft model.
   • In our hospital, we investigated the prognostic significance of TAM in MSI-high gastric cancers using IHC. (Kim et al. 2015) CD68 and CD163 were used as markers for total infiltrating macrophages and M2-polarized macrophages, respectively. The density of CD68+ or CD163+ TAMs in four different areas (epithelial and stromal compartments of both the tumor center and invasive front) were analyzed in 143 cases of MSI-high advanced gastric cancers.

   In multivariate survival analysis, CD163+ TAMs in four combined areas, stromal and epithelial compartments of both tumor center and invasive front were independent prognostic indicator in MSI-high gastric cancers.

   • Polarized CD163+ TAM was also reported to be associated with increased angiogenesis and CXCL12 expression in gastric cancer (Park et al. 2015)
   • We investigated the role of cytokine and angiogenic factors in the gastric cancer (Ock et al. 2015, Ock et al. 2016). High serum level of M-CSF is associated with poor survival outcome in gastric cancer patients who received standard 1st-line chemotherapy.

6. Potential of combination of PD1 inhibitor and CSF-1 inhibitor

   • Blockade of colony-stimulating factor-1 (CSF-1) limits macrophage infiltration and improves response of mammary carcinomas to chemotherapy. ( Ruffell B et al. Cancer Cell 2014)
   • In BRAF mutant melanoma model, CSF1R inhibitor (PLX3397) reduced the recruitment of CD11bC Gr1lo and CD11bC Gr1int M2-like macrophages, but this was accompanied by an accumulation of CD11bC Gr1hi cells. PDL1 expression on remaining myeloid cells potentially dampened the antitumor efficacy of BRAF inhibitor (PLX3397) and PLX4720 in combination, since PD1/PDL1 axis blockade improved outcome. (Ngiow SF et al. 2015)
   • In pancreatic cancer model, inhibiting signaling by the myeloid growth factor receptor CSF1R can functionally reprogram macrophage responses that enhance antigen presentation and productive antitumor T-cell responses. (Zhu Y et al. 2014) However, CSF1R blockade also upregulated T-cell checkpoint molecules, including PDL1 and CTLA4, thereby restraining beneficial therapeutic effects.
   • In these cases, combination of CSF1R blockade and PD1 or CTLA4 inhibitor potently decreased tumor progression.

\<Trial design and plan> Each dosing interval will be 3 weeks.

1. Before 1st dosing of MCS110, 1st fresh tumor biopsy will be done.
2. After 1st dosing of MCS110 monotherapy, 2nd fresh tumor biopsy will be done
3. Then, combination of MCS110 with PDR001 will be initiated.
4. After 1st dosing of MCS110/PDR001 combination, 3rd fresh tumor biopsy will be done.
5. After that, the MCS110/PDR001 combination will be delivered to the patients until disease progression, intolerable toxicity, or patient's consent withdrawal.
6. At the time of disease progression, fresh tumor tissue will be obtained if possible (optional).
7. In each tumor biopsy time point, blood sampling will be accompanied.
8. Tumor response evaluation will be done every 2 cycles of MCS110/PDR001 combination treatment.

Translational research that will be performed. The description of biomarker changes will be the main purpose of this biomarker study. Therefore, the translational research is the main interest of this study.

The candidate biomarkers are as below, which was adopted from the MCS110Z2102 clinical trial. However, the exact analysis items will be chosen based on the tumor tissue amount and developing science during study period. The main focus will be on PDL1, TAM, TIL.