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Active, not recruitingNCT03644563MOUTHUpdated Sep 11, 2025

Men and Women Offering Understanding of Throat HPV

An observational study in Oropharyngeal Cancer and Human Papilloma Virus, sponsored by Johns Hopkins University. Active, not recruiting at 1 site in United States. Open to participants aged 18 Years and older, including healthy volunteers. Per ClinicalTrials.gov, last updated 2025-09-11.

Sponsored by Johns Hopkins University · Observational

Study type
Observational
Model
Cohort
Time perspective
Prospective
Enrollment
1,500
Ages
18 Years and older
Sex
All
01

Study summary

This study will screen people for oncogenic oral Human Papillomavirus (HPV) infection and antibodies to form a cohort of people who may be at increased risk of HPV-oropharyngeal cancer (HPV-OPC). The investigators will follow these individuals prospectively to evaluate oncogenic oral HPV persistence, risk factors, and biomarkers for persistence.

Read the detailed description

This study will provide one of the first estimates of long-term oral HPV natural history, and the effect of biologic and behavioral risk factors, including HIV, on this natural history. Phase 1 of the study will screen approximately 1500 people for oncogenic oral HPV biomarkers. Phase 2 of the study will follow only those subjects with oncogenic oral HPV infection and/or HPV serum oncogene antibodies from Phase 1 (and those previously identified as having oncogenic oral HPV infection in a previous study) with annual follow-up for oncogenic oral HPV persistence.

Understanding persistent oncogenic oral HPV infection is the focus of this study. Understanding which factors drive oral HPV infection to become persistent or progress to malignancy is critical to determine who is at high risk for oropharyngeal cancer and may benefit from screening and prevention. It is presumed that persistent oncogenic oral HPV infections are necessary for progression to HPV-OPC.

The study is led by Dr. Amber D'Souza and Dr Carole Fakhry (Johns Hopkins) and participants are being enrolled in Baltimore MD (Johns Hopkins) and in New York (Mt. Sinai).

02

Conditions studied

  • Oropharyngeal Cancer
  • Human Papilloma Virus

Keywords

  • Oral HPV
  • Persistent infection
  • Oropharyngeal cancer (OPC)
  • HPV antibody
  • HPV Screening Study
03

Who can participate

Ages eligible
18 Years and older
Sexes eligible
All
Accepts healthy volunteers
Yes
Sampling method
Non-probability sample

Study population

Target sample size: 1325 (phase 1), \~250 (phase 2) Inclusion of women and minorities: Primary enrollment is restricted to men, given their higher risk of infection (see eligibility criteria). However, the investigators expect to enroll women with cervical dysplasia and partners of patients with HPV-related cancer (\~125 expected women enrollees). Enrollment is across many different sources (geographically, and by clinic type), and is inclusive of diverse racial and ethnic backgrounds. Enrollment is not stratified by race but the investigators expect to enroll at least 175 minorities in the study.

Eligibility criteria

Participants in the Phase 1 Screening

Inclusion criteria:

In order to be eligible to participate in this study, an individual must meet all of the following criteria:

  • 18 and older (enrollment at most sites will be restricted to 30 and older since this demographic has the highest prevalence of infection; however, individuals and/or partners of someone with an HPV-related cancer, for example, are at increased risk so will be enrolled in the larger age range).
  • Willing to be contacted to arrange follow-up visits, if determined to be eligible for phase 2
  • Ability to understand and the willingness to sign a written informed consent document

In addition, individuals must meet at least one of the following criteria:

  • Male, aged 30 and older, with 2 or more lifetime oral sex
  • History of anal or genital dysplasia or cancer
  • Partners of someone with an HPV-related cancer (HPV-positive OPC, anal cancer or genital cancer)
  • Known oncogenic HPV-positive biomarker from prior studies or testing, regardless of number of partners.

Exclusion criteria:

An individual who meets any of the following criteria will be excluded from participation in this study:

  • Presence of medical or psychiatric condition affecting ability to give voluntary, informed consent.
  • Participants who do not speak English cannot enroll because the consent and study survey are only available in English. However, if there is a hearing, literacy or minor fluency issue and the participant requests assistance from a family member or the study coordinator, this individual may still enroll and receive this assistance to ensure they fully hear and understand everything being asked.
  • History of head and neck cancer

Participants in the Phase 2 Follow-Up

Inclusion criteria:

  • Oncogenic oral HPV infection and/or HPV serum oncogenic antibodies detected in phase 1
  • Willingness to complete annual follow up visits

Exclusion criteria:

  • Unable to complete annual follow up visits
04

Study design

Observational model
Cohort
Time perspective
Prospective
Enrollment
1,500 participants (estimated)
Target follow-up
4 Years
Patient registry
Yes
Biospecimen retention
Samples with dna

Groups and cohorts

  • Follow-up

    Those subjects with oncogenic oral HPV infection and/or HPV oncogene serum antibodies from screening.

05

What researchers measure

Primary outcomes

  1. Oncogenic oral HPV infection present or absent in oral rinse sample

    To explore the effects of biologic (microbiome, oral immune response, biologic sex) risk factors for oncogenic oral HPV persistence

    Time frame: Baseline to end of four year follow-up and data abstraction/linkage.

Secondary outcomes

  1. HPV16 E6 antibodies present or absent in serum sample

    To explore whether E6 and E7 seropositivity (to HPV16 or any oncogenic HPV) are markers for oncogenic oral HPV persistence among high-risk groups

    Time frame: Baseline to end of four year follow-up and data abstraction/linkage.

06

Study locations

1 site
  • Johns Hopkins Hospital
    Baltimore, Maryland 21205, United States
07

References and documents

Publications

  • D'Souza G, Clemens G, Troy T, Castillo RG, Struijk L, Waterboer T, Bender N, Pierorazio PM, Best SR, Strickler H, Wiley DJ, Haddad RI, Posner M, Fakhry C. Evaluating the Utility and Prevalence of HPV Biomarkers in Oral Rinses and Serology for HPV-related Oropharyngeal Cancer. Cancer Prev Res (Phila). 2019 Oct;12(10):689-700. doi: 10.1158/1940-6207.CAPR-19-0185. Epub 2019 Aug 16. PubMed 31420362 ↗
  • D'Souza G, Tewari SR, Troy T, Waterboer T, Struijk L, Castillo R, Wright H, Shen M, Miles B, Johansson M, Robbins HA, Fakhry C. Prevalence of oral and blood oncogenic human papillomavirus biomarkers among an enriched screening population: Baseline results of the MOUTH study. Cancer. 2023 Aug 1;129(15):2373-2384. doi: 10.1002/cncr.34783. Epub 2023 Apr 9. PubMed 37032449 ↗
  • D'Souza G, Tewari SR, Troy T, Webster-Cyriaque J, Wiley DJ, Lahiri CD, Palella FJ, Gillison ML, Strickler HD, Struijk L, Waterboer T, Ho K, Kwait J, Lazar J, Weber KM, Fakhry C. Oncogenic Oral Human Papillomavirus Clearance Patterns over 10 Years. Cancer Epidemiol Biomarkers Prev. 2024 Apr 3;33(4):516-524. doi: 10.1158/1055-9965.EPI-23-1272. PubMed 38294704 ↗

Individual participant data

Plan to share: No

08

Registry details

Key details

Study ID
NCT03644563
Lead sponsor
Johns Hopkins University
Collaborators
Icahn School of Medicine at Mount Sinai, National Institute of Dental and Craniofacial Research (NIDCR)
Responsible party
Sponsor
First posted
Aug 23, 2018
Start date
Apr 3, 2017
Primary completion
Sep 2026 (estimated)
Completion
Jun 2028 (estimated)
Last update
Sep 11, 2025

Study contacts

Amber D'Souza, PhD
principal investigator · Bloomberg Johns Hopkins School of Public Health

Oversight

Data monitoring committee
Yes
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

Not currently enrolling

This study is active, not recruiting, as verified in Sep 2025. You cannot join it, but the record below documents what was studied.

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