An observational study in Athletes, Respiratory Infection and Cytokines, sponsored by Research Institute of Epidemiology, Microbiology and Infectious Diseases, Uzbekistan. Suspended. Open to participants aged 17 Years to 28 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2023-03-02.
Sponsored by Research Institute of Epidemiology, Microbiology and Infectious Diseases, Uzbekistan · Observational
The purpose of the study is to determine vitamin D (VD) levels and upper respiratory tract infections (URTI) morbidity among elite athletes engaged in water sports in Uzbekistan. Serum levels of 25(OH) VD and TNF-α, IFN-γ, IL-4 and IL-6 will be detected by ELISA technique among elite athletes and control population in spring and autumn.
Study center The prospective diagnostic study will be conducted on the basis of Uzbek State University of Physical Education and Sport and Research Institute of Epidemiology, Microbiology and Infectious Diseases, Tashkent, Uzbekistan during the period from January 2017 till January 2019.
Both informed and written consents will be obtained from the athletes and healthy individuals.
Study participants Aproximately 40 elite athletes engaged in water sport and 60 healthy individuals will be included to the study.
All the participants will be residents of Uzbekistan.
Serological tests Five milliliters of peripheral venous blood will be taken (after 8-12 hours of fasting) from each participant and will be collected into Human Tube Serum Gel - C/A for ELISA. All blood samples will be collected in August and January.
Serum levels of 25(OH) VD and TNF-α, IFN-γ, IL-4 and IL-6 will be detected by ELISA technique.
Enzyme immunoassay for the quantitative measurement of total 25-OH-Vitamin D (Vitamin D2 and D3) in human serum.
Solid phase enzyme-linked immunosorbent assay (ELISA) based on the competition principle. An unknown amount of antigen present in the sample and a fixed amount of enzyme labelled antigen compete for the binding sites of the antibodies coated onto the wells. After incubation the wells are washed to stop the competition reaction. After the substrate reaction the intensity of the developed colour is inversely proportional to the amount of the antigen in the sample. Results of samples can be determined directly using the standard curve.
A sandwich enzyme immunoassay for in vitro quantitative measurement of TNFa The microplate provided in this kit has been pre-coated with an antibody specific to TNFa. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to TNFa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNFa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TNFa in the samples is then determined by comparing the O.D. of the samples to the standard curve.
IFN-γ, IL-4 and IL-6 will be detected and measured by the same enzyme immunoassay method presented above.
Data analysis will be performed with the program Origin 6.1 (OriginLab, Northampton, MA).
1,031 studies on the registry are indexed under Respiratory Tract Infections; 144 are open to participants now.
This study's enrollment of 100 is below the median of 300 across 295 observational studies indexed under Respiratory Tract Infections.
Browse Respiratory Tract Infections studies →Research Institute of Epidemiology, Microbiology and Infectious Diseases, Uzbekistan is the lead sponsor of 8 studies on the registry; 1 is open to participants now.
Counted across the registry records on this site, refreshed daily.
Healthy individuals Swimmers to distance Synchronized swimmers
Exclusion Criteria:
Individuals without any complaints and chronic diseases
Diagnostic Test: Serologic tests of blood samples
Elite athletes - swimmers to distance of national team of Uzbekistan
Diagnostic Test: Serologic tests of blood samples
Elite athletes - swimmers of national team of Uzbekistan
Diagnostic Test: Serologic tests of blood samples
Five milliliters of peripheral venous blood will be (after 8-12 hours of fasting) from each participant and will be collected into Human Tube Serum Gel - C/A for ELISA. All blood samples will be collected in August and January. Serum levels of 25(OH) VD and TNF-α, IFN-γ, IL-4 and IL-6 will be detected by ELISA technique
Vitamin D level in elite athletes
In this study we expect to determine of serum level of vitamin D in elite athletes of Uzbekistan
Time frame: up to 36 months
Correlation between cytokine status (TNF-α, IFN-γ, IL-4 and IL-6 ) and vitamin D level among elite athletes
In this study we expect to find association between cytokine status and vitamin D level in elite athletes.
Time frame: up to 36 months
Incidence of upper respiratory infection among elite athletes with vitamin D deficiency/insufficiency/sufficiency
In this study we expect to find a role of vitamin D in development of upper respiratory infection among elite athletes.
Time frame: up to 36 months
No study locations are listed for this record.
Plan to share: Undecided
This study is suspended, as verified in Mar 2023. You cannot join it, but the record below documents what was studied.
Get an email when the registry record changes — status, dates, results — or when someone posts here.
Sign in to followQuestions and observations about this study, from anyone following it. Not medical advice, and not a channel to the study team — their contact details are on the registry record.
Sign in to join the discussion. Reading takes no account; posting does. You choose a display name, and a pseudonym is the default.
Nothing here yet. If you are running this trial, taking part in it, or weighing whether to, this is the place to say so.
Research Institute of Epidemiology, Microbiology and Infectious Diseases, Uzbekistan