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CompletedNCT03438942WiMKoUpdated Apr 5, 2022

Influence of Iron and Foliate Transporters on Bioavailability of These Micronutrients in the Organism

An interventional study of Folic acid and iron supplementation and Control group in Nutrition Disorders, sponsored by Poznan University of Life Sciences. Completed at 1 site in Poland. Open to female participants aged 18 Years to 35 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2022-04-05.

Sponsored by Poznan University of Life Sciences · Not applicable, Interventional, and Diagnostic

Phase
Not applicable
Study type
Interventional
Enrollment
200
Allocation
Non-randomized
Ages
18 Years to 35 Years
Sex
Female
01

Study summary

The aim of the study is to determine the factors affecting the bioavailability of iron and folic acid during the simultaneous use of iron supplements and folic acid supplements in non-pregnant women of childbearing age.

Read the detailed description

Scientists in the field of nutrition and gynecology and obstetrics have been paying attention to the need of use iron and folate supplements in women of childbearing age. Increased intake of micronutrients in the diet or the use of supplementation does not always bring the expected results, what depends on factors that affect the bioavailability of minerals and vitamins. On the one hand these factors are related to the properties of food intake, e.g. to the presence of other nutrients that can increase or decrease the absorption of folates and iron (eg vitamin C, phytates), and on the other hand the functioning of the body itself, which to a certain extent is determined by genetic factors (e.g., genetically determined reduced activity of methylenetetrahydrofolate reductase [MTHFR], which reduces the bioavailability of folic acid).

The study aims:

  • assessment of the effect of simultaneous supplementation with iron and folate on:

    • parameters of the iron metabolism
    • parameters of the folate metabolism
  • the estimation of gene polymorphisms encoding proteins transporting iron and folate
  • obtaining information whether the polymorphism of genes encoding proteins transporting iron and folate is related to the effectiveness of the iron and folic acid supplementation used in non-pregnant women of childbearing age.

In 200 non-pregnant women of childbearing age a blood level of iron and folic acid will be determined. Subjects with low level of blood iron and folic acid will be supplemented with oral iron and folic acid for 3 months. Subjects with proper level of blood iron and folic acid will be a control group. At baseline and at completion of the study (after 3 months) fasting blood will be collected and abovementioned parameters will be estimated.

02

Conditions studied

  • Nutrition Disorders

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Keywords

  • iron
  • folate
  • childbearing age
  • bioavailability
03

Who can participate

Ages eligible
18 Years to 35 Years
Sexes eligible
Female
Accepts healthy volunteers
Yes

Inclusion criteria

  • age 18 to 35 years
  • stable body weight (\< 3 kg self-reported change during the previous three months)
  • written informed consent to participate in the study,
  • regular menstrual period;
  • regular diet

Exclusion criteria

Exclusion Criteria:

  • history of use of any dietary supplements within the one month prior to the study containing iron or folic acid
  • clinically relevant acute or chronic inflammatory disease in the respiratory, gastrointestinal or genitourinary system or in the mouth, throat, paranasal sinuses and / or connective tissue disease, arthritis;
  • simultaneous participation in a study that affects body weight or use of diet / medication / nutritional behaviors affecting body weight;
  • a history of infection in the month prior to the study
  • nicotine, drug or alcohol abuse
  • vegetarian diet;
  • pregnancy or lactation
  • other condition that, in the opinion of the investigators, would make participation not in the best interest of the patient or could prevent, limit, or confound the study results
04

Study design

Phase
Not applicable
Primary purpose
Diagnostic
Allocation
Non-randomized
Intervention model
Parallel assignment
Masking
None (open label)
Enrollment
200 participants (actual)

Study arms

  • Experimental
    Folic acid and iron supplementation

    Individuals with low level of blood folic acid and iron- will receive folic acid and iron supplementation daily, for 3 months

    Dietary Supplement: Folic acid and iron supplementation

  • Active comparator
    Control group

    Individuals with proper level of blood folic acid and iron- will not receive folic acid and iron supplementation daily, for 3 months

    Dietary Supplement: Control group

Interventions

  • Dietary supplementFolic acid and iron supplementation

    Individuals with low level of blood folic acid and iron- will receive folic acid and iron supplementation daily, for 3 months

  • Dietary supplementControl group

    Individuals with proper level of blood folic acid and iron- will not receive folic acid and iron supplementation daily, for 3 months

05

What researchers measure

Primary outcomes

  1. folic acid concentrations at baseline

    blood folic acid concentrations at baseline

    Time frame: At the baseline

  2. folic acid concentrations after 3 months of treatment

    blood folic acid concentrations after 3 months of treatment

    Time frame: after 3 months of treatment

  3. iron concentrations at baseline

    blood iron concentrations at baseline

    Time frame: At the baseline

  4. iron concentrations after 3 months of treatment

    blood ron concentrations after 3 months of treatment

    Time frame: after 3 months of treatment

Secondary outcomes

  1. blood pressure

    blood pressure

    Time frame: At the baseline

  2. body mass

    body mass

    Time frame: At the baseline

  3. body height

    body height

    Time frame: At the baseline

  4. Body % fat

    Fat tissue % estimated with air displacement plethysmography

    Time frame: At the baseline

  5. Body % muscle

    Muscle tissue % estimated with BodPod (air displacement plethysmography)

    Time frame: At the baseline

  6. ferritin at the baseline

    serum ferritin concentration at the baseline

    Time frame: At the baseline

  7. ferritin after 3 months of treatment

    serum ferritin concentration after 3 months of treatment

    Time frame: after 3 months of treatment

  8. hepcidin at the baseline

    serum hepcidin concentration at the baseline

    Time frame: At the baseline

  9. hepcidin after 3 months of treatment

    serum hepcidin concentration after 3 months of treatment

    Time frame: after 3 months of treatment

  10. homocysteine at the baseline

    serum homocysteine concentration at the baseline

    Time frame: At the baseline

  11. homocysteine after 3 months of treatment

    serum homocysteine concentration after 3 months of treatment

    Time frame: after 3 months of treatment

  12. total iron-binding capacity (TIBC) at the baseline

    Blood total iron-binding capacity at the baseline

    Time frame: At the baseline

  13. TIBC after 3 months of treatment

    Blood total iron-binding capacity after 3 months of treatment

    Time frame: after 3 months of treatment

  14. divalent metal transporter 1 (DMT1) gene polymorphisms

    polymorphisms of gene encoding divalent metal transporter 1

    Time frame: At the baseline

  15. transferrin receptor 2 (TfR2) gene polymorphisms

    polymorphisms of gene encoding transferrin receptor 2

    Time frame: At the baseline

  16. proton-coupled folate transporter (PCFT) gene polymorphisms

    polymorphisms of gene encoding proton-coupled folate transporter

    Time frame: At the baseline

  17. reduced folate carrier (RFC) gene polymorphisms

    polymorphisms of gene encoding reduced folate carrier

    Time frame: At the baseline

06

Study locations

1 site
  • Poznan University of Life Sciences
    Poznań, 60-624, Poland
07

Registry details

Key details

Study ID
NCT03438942
Lead sponsor
Poznan University of Life Sciences
Responsible party
Joanna Suliburska (assistant professor, Poznan University of Life Sciences) — Principal investigator
First posted
Feb 20, 2018
Start date
Sep 30, 2018
Primary completion
Sep 30, 2021
Completion
Dec 30, 2021
Last update
Apr 5, 2022

Study contacts

Joanna Suliburska, Ass Prof
principal investigator · Institute of Human Nutrition and Dietetics, Poznań University of Life Sciences

Oversight

Data monitoring committee
No
FDA-regulated drug
No
FDA-regulated device
No
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