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CompletedNCT03401541Updated Jun 4, 2020

25-Hydroxyvitamin D Pharmacokinetic Study

An Early Phase 1 interventional study of Calcifediol and Calciferol in Vitamin D Deficiency and Fat Malabsorption, sponsored by Boston University. Completed at 1 site in United States. Open to participants aged 18 Years and older, including healthy volunteers. Per ClinicalTrials.gov, last updated 2020-06-04.

Sponsored by Boston University · Early Phase 1, Interventional, and Treatment

Phase
Early Phase 1
Study type
Interventional
Enrollment
16
Allocation
Randomized
Ages
18 Years and older
Sex
All
01

Study summary

To evaluate the pharmacokinetic parameters of orally administered 25-hydroxyvitamin D3 [25(OH)D3] and vitamin D3 from the corresponding serum concentration-time curves in healthy adults and adults with a history of intestinal malabsorption.

Read the detailed description

Patients with a history of intestinal malabsorption (n=10) and healthy volunteers (n=10); (of both sexes and all ethnicities) will be recruited in this randomized double-blind crossover study. All subjects will be prescreened for 25(OH)D levels to include subjects with 25(OH)D\<30 ng/ml. The subjects will be randomized to receive a single oral dose of 900 µg of either vitamin D3 or 25(OH)D3. Blood samples will be taken at baseline and at 2,4,6,8,12 hours and days 1,2,3, 7 and 14. After a washout period of at least 14 days (2 weeks) the subjects will receive in a double blinded manner a single oral dose of 900 µg of either 25(OH)D3 or vitamin D3 (depending on which one they took in the randomization) any time after the washout. Blood samples will be taken at baseline and at 2,4,6,8,12 hours and days 1,2,3,7, 14.

Serum levels of calcium, phosphorus, albumin, creatinine, intact parathyroid hormone (iPTH) will be obtained at baseline and at day 14 (or within a reasonable time if unable to come in on the exact day). Vitamin D and 25(OH)D will be determined on all the blood samples collected at the various times.

02

Conditions studied

  • Vitamin D Deficiency
  • Fat Malabsorption

Keywords

  • Calcifediol (25(OH)D)
  • Calciferol (Vitamin D)
03

Who can participate

Ages eligible
18 Years and older
Sexes eligible
All
Accepts healthy volunteers
Yes

Inclusion criteria

  • Adult male or female with history of intestinal fat-malabsorption with confirmed diagnosis
  • Taking vitamin D (ergocalciferol or cholecalciferol). Subjects taking vitamin D supplement of more than 2000 IUs (50 micrograms)/day) that may interfere with study endpoints must be willing and able to discontinue use of these supplements for the duration of the study and allow for at least a 14-day washout prior to prescreening and enrollment.
  • Women of child bearing potential must be on birth control and not pregnant based on a negative pregnancy test at baseline for each of the 2 pharmacokinetic studies.
  • Serum total 25(OH)D \< 30 ng/mL

Exclusion criteria

Exclusion Criteria:

  • Unwilling to discontinue use of supplemental dose of vitamin D2 (ergocalciferol) or vitamin D3 (cholecalciferol) daily equivalent (2,000 IU/50 μg) 1 week or less prior to randomization and during the study
  • On medications that can affect vitamin D metabolism including steroids such as prednisone, anti-seizure medications and medications to treat HIV.
  • Tanning in a tanning bed at least one week before the study and throughout the duration of this study.
  • Anyone anticipating going on holiday (where exposure to sun is imminent) 1 week before or during the entire study.
  • Participation in the study or any reason which, in the opinion of the investigator makes adherence to a treatment or follow-up schedule unlikely
  • History of elevated serum calcium
  • Chronic hepatic or renal failure
  • Subjects with a history of an adverse reaction to orally administered vitamin D.
  • Inability to give informed consent
04

Study design

Phase
Early Phase 1
Primary purpose
Treatment
Allocation
Randomized
Intervention model
Crossover assignment
Masking
Double (Participant, Investigator)
Enrollment
16 participants (actual)

Study arms

  • Experimental
    Fat-Mal, calcifediol then calciferol

    Participants with diagnosed fat malabsorption syndrome will initially receive one capsule of calcifediol for the first round of blood draws and then receive one capsule of calciferol for the second round.

    Drug: Calcifediol · Drug: Calciferol

  • Experimental
    Fat-Mal, calciferol then calcifediol

    Participants with diagnosed fat malabsorption syndrome will initially receive one capsule of calciferol for the first round of blood draws and then receive one capsule of calcifediol for the second round.

    Drug: Calcifediol · Drug: Calciferol

  • Experimental
    Non Fat-Mal, calcifediol then calciferol

    Participants that do not have a diagnosed fat malabsorption syndrome will initially receive one capsule of calcifediol for the first round of blood draws and thenreceive one capsule of calciferol for the second round.

    Drug: Calcifediol · Drug: Calciferol

  • Experimental
    Non Fat-Mal, calciferol then calcifediol

    Participants that do not have a diagnosed fat malabsorption syndrome will initially receive one capsule of calciferol for the first round of blood draws and then receive one capsule of calcifediol for the second round.

    Drug: Calcifediol · Drug: Calciferol

Interventions

  • DrugCalcifediol

    One capsule of 900 micrograms of 25(OH)D

    Also known as: 25(OH)D

  • DrugCalciferol

    One capsule of 900 micrograms of Vitamin D

    Also known as: Vitamin D

05

What researchers measure

Primary outcomes

  1. Change in Serum vitamin D status

    The serum vitamin D status (pg/mL) will be determined at multiple time points secondary to receiving a single dose of either calciferol or calcifediol. We will obtain 11 time points after each drug administration which will allow us to observe the fluctuation in vitamin D status after receiving the study drug.

    Time frame: 2 weeks after administration

  2. Change in 25-Hydroxyvitamin D Status

    The serum 25(OH)D status (ng/mL) will be determined at multiple time points secondary to receiving a single dose of either calciferol or calcifediol. We will obtain 11 time points after each drug administration which will allow us to observe the fluctuation in 25(OH)D status after receiving the study drug.

    Time frame: 2 weeks after administration

Secondary outcomes

  1. Serum Calcium Levels

    Calcium levels will be drawn at baseline and at the end of each intervention period. This measure will allow us to ensure no change to baseline levels after receiving the study drug.

    Time frame: Baseline and completion of each intervention period (2 weeks)

  2. Serum Phosphorus Levels

    Phosphorus levels will be drawn at baseline and at the end of each intervention period. This measure will allow us to ensure no change to baseline levels after receiving the study drug.

    Time frame: Baseline and completion of each intervention period (2 weeks)

  3. Serum Albumin Levels

    Albumin levels will be drawn at baseline and at the end of each intervention period. This measure will allow us to ensure no change to baseline levels after receiving the study drug.

    Time frame: Baseline and completion of each intervention period (2 weeks)

  4. Serum Creatinine levels

    Creatinine levels will be drawn at baseline and at the end of each intervention period. This measure will allow us to ensure no change to baseline levels after receiving the study drug.

    Time frame: Baseline and completion of each intervention period (2 weeks)

  5. Serum intact parathyroid hormone levels

    iPTH levels will be drawn at baseline and at the end of each intervention period. This measure will allow us to ensure no change to baseline levels after receiving the study drug.

    Time frame: Baseline and completion of each intervention period (2 weeks)

06

Study locations

1 site
  • Solomon Carter Fuller Mental Health Center
    Boston, Massachusetts 02118, United States
07

References and documents

Publications

  • Charoenngam N, Kalajian TA, Shirvani A, Yoon GH, Desai S, McCarthy A, Apovian CM, Holick MF. A pilot-randomized, double-blind crossover trial to evaluate the pharmacokinetics of orally administered 25-hydroxyvitamin D3 and vitamin D3 in healthy adults with differing BMI and in adults with intestinal malabsorption. Am J Clin Nutr. 2021 Sep 1;114(3):1189-1199. doi: 10.1093/ajcn/nqab123. PubMed 34008842 ↗

Individual participant data

Plan to share: Yes — Data will be available upon reasonable request to mfholick@bu.edu.

Supporting information: Study protocol, Sap, Csr

08

Registry details

Key details

Study ID
NCT03401541
Lead sponsor
Boston University
Responsible party
Sponsor
First posted
Jan 17, 2018
Start date
Oct 1, 2018
Primary completion
Apr 1, 2019
Completion
Nov 1, 2019
Last update
Jun 4, 2020

Study contacts

Michael F Holick, MD, PhD
principal investigator · Boston University

Oversight

Data monitoring committee
Yes
FDA-regulated drug
Yes
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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