An interventional study of second masturbation in Embryo Development, sponsored by Universitair Ziekenhuis Brussel. Completed at 1 site in Belgium. Open to participants aged 18 Years and older, including healthy volunteers. Per ClinicalTrials.gov, last updated 2024-09-19.
Sponsored by Universitair Ziekenhuis Brussel · Not applicable, Interventional, and Diagnostic
The aim the investigators study is to analyze the embryo development if oocytes of the participants are injected with sperm from a longer or shorter (2 hours) abstinence duration.
Participants undergoing an IVF treatment, are asked to respect an abstinence duration of 2-7 days. This is to obtain a sufficient number of motile sperm. A recent investigation, analyzed the quality (number and motility) of a sperm sample produced after an abstinence duration of 4 to 7 days, followed by a second sperm sample 2 hours later. The second sample (after 2 hours) was able to produce sperm cells with a higher motility. Not only has this short abstinence duration of 2 hours a positive effect on the motility, it will also reduce the sperm DNA damage, which again has a positive effect on embryo development. Because participants need gametes of high quality to obtain embryos with a high quality, the investigators want to investigate what the effect is of abstinence duration on embryo development.
Exclusion Criteria:
Patients follow the normal abstinence duration as asked by the physician (2-7 days). This sperm will be used to inject half of the oocytes Intervention = second sperm sample
Other: second masturbation
Patients will be asked to produce a second sperm sample, 2 hours after the first. This sperm will be used to inject the other half of the oocytes. Intervention = second sperm sample
Other: second masturbation
Husbands will be asked to produce a second sperm sample in the masturbatorium
Embryo development (blastocyst stage)
embryo development 5/6 of the preimplantation development
Time frame: 1 year
Embryo development (cleavage stage)
embryo development on day 3 of the preimplantation development
Time frame: 1 year
Fertilization
number of fertilized oocytes per number of mature oocytes
Time frame: 1 year
Utilization rate
number of embryos used for transfer + cryopreserved for the patient
Time frame: 1 year
Sperm quality
concentration and motility
Time frame: 1 year
Sperm DNA fragmentation
analysis of non-viable sperm in the two samples
Time frame: 1 year
Clinical Pregnancies
pregnancies with a fetal heart beat
Time frame: 1 year
Plan to share: No — No data available for researchers others than the ones involved in the study
No publications or documents are linked to this record.
This study is completed, as verified in Sep 2024. You cannot join it, but the record below documents what was studied.
Get an email when the registry record changes — status, dates, results — or when someone posts here.
Sign in to followQuestions and observations about this study, from anyone following it. Not medical advice, and not a channel to the study team — their contact details are on the registry record.
Sign in to join the discussion. Reading takes no account; posting does. You choose a display name, and a pseudonym is the default.
Nothing here yet. If you are running this trial, taking part in it, or weighing whether to, this is the place to say so.
Universitair Ziekenhuis Brussel