CClinicalTrials.gg
CompletedNCT03285295Updated Oct 9, 2019Results posted

Abbott Laboratories Alinity s Blood Screening Assays - Clinical Evaluation Protocol

An interventional study of Alinity s HBsAg and Alinity s HBsAg Confirmatory Assay and Alinity s HTLV I/II Assay in Healthy, sponsored by Abbott Diagnostics Division. Completed at 5 sites in United States. Open to participants aged 16 Years and older, including healthy volunteers. Per ClinicalTrials.gov, last updated 2019-10-09.

Sponsored by Abbott Diagnostics Division · Not applicable, Interventional, and Screening

Phase
Not applicable
Study type
Interventional
Enrollment
106,881
Allocation
Not applicable
Ages
16 Years and older
Sex
All
01

Study summary

The objective of this study is to demonstrate the performance and intended use of each of the Alinity s investigational assays in a donor screening environment using clinical specimens to evaluate assay performance characteristics. A comparison of assay performance will be done versus the Food and Drug Administration (FDA) licensed assays. The data will be used to support regulatory submissions and/or publications.

Read the detailed description

This study evaluates Alinity s investigational assays using the Alinity s System. The Alinity s System is a high-volume, automated, blood-screening analyzer that is designed to determine the presence of specific antigens and antibodies by using chemiluminescent microparticle immunoassay (CMIA) detection technology. The system performs high-throughput routine and stat processing that features continuous access and automated retesting.

The Alinity s System is used for infectious disease marker testing in blood-screening and plasma laboratories for the following assays: Anti-HBc, Anti-HCV, HTLV I/II, Chagas, HBsAg, HBsAg Confirmatory and HIV Ag/Ab Combo.

02

Conditions studied

  • Healthy
03

Who can participate

Ages eligible
16 Years and older
Sexes eligible
All
Accepts healthy volunteers
Yes

Inclusion criteria

  • Healthy donors who consented to participate in the study.

Exclusion criteria

Exclusion Criteria:

  • For testing with the Alinity s Chagas assay, exclude donors that have been screened on a previous donation using a licensed test for antibodies to T cruzi.

Note: A subject may participate at different times during the study for separate assay studies, but each subject should be represented only once for each assay.

The protocol does not define an age limit. A donor must be at least 17 years old to donate to the general blood supply, or 16 years old with parental/guardian consent, if allowed by state law.

04

Study design

Phase
Not applicable
Primary purpose
Screening
Allocation
Not applicable
Intervention model
Single group
Masking
None (open label)
Enrollment
106,881 participants (actual)

Study arms

  • Experimental
    Screening

    All subjects will be tested with at least one of the investigational Alinity s assays (Anti-HBc, Anti-HCV, HTLV I/II, Chagas, HBsAg, HBsAg Confirmatory, HIV Ag/Ab Combo) on Alinity s system.

    Device: Alinity s HBsAg and Alinity s HBsAg Confirmatory Assay · Device: Alinity s HTLV I/II Assay · Device: Alinity s Anti-HCV Assay · Device: Alinity s HIV Ag/Ab Combo Assay · Device: Alinity s Anti-HBc Assay · Device: Alinity s Chagas Assay

Interventions

  • DeviceAlinity s HBsAg and Alinity s HBsAg Confirmatory Assay

    For all donor specimens with investigational Alinity s HBsAg and Alinity s HBsAg Confirmatory results that are discordant with final status after supplemental testing, an attempt will be made to obtain a follow-up whole blood specimen approximately 4 to 6 weeks after initial donation for further analysis.

  • DeviceAlinity s HTLV I/II Assay

    For all donor specimens with investigational Alinity s HTLV I/II results that are discordant with final status after supplemental testing, an attempt will be made to obtain a follow-up whole blood specimen approximately 4 to 6 weeks after initial donation for further analysis.

  • DeviceAlinity s Anti-HCV Assay

    For all donor specimens with investigational Alinity s Anti-HCV results that are discordant with final status after supplemental testing, an attempt will be made to obtain a follow-up whole blood specimen approximately 4 to 6 weeks after initial donation for further analysis.

  • DeviceAlinity s HIV Ag/Ab Combo Assay

    For all donor specimens with investigational Alinity s HIV Ag/Ab Combo results that are discordant with final status after supplemental testing, an attempt will be made to obtain a follow-up whole blood specimen approximately 4 to 6 weeks after initial donation for further analysis.

  • DeviceAlinity s Anti-HBc Assay

    For all donor specimens with investigational Alinity s Anti-HBc results that are discordant with final status after supplemental testing, an attempt will be made to obtain a follow-up whole blood specimen approximately 4 to 6 weeks after initial donation for further analysis.

  • DeviceAlinity s Chagas Assay

    For all donor specimens with investigational Alinity s Chagas results that are discordant with final status after supplemental testing, an attempt will be made to obtain a follow-up whole blood specimen approximately 4 to 6 weeks after initial donation for further analysis.

05

What researchers measure

Primary outcomes

  1. Alinity s HBsAg Assay Specificity

    Total of 16993 serum and plasma specimens from whole blood donors as well as plasmapheresis specimens were tested with Alinity s HBsAg Assay. Repeatedly reactive specimens were further tested with Alinity s HBsAg Confirmatory and supplemental assays, if required.

    Time frame: 10 months

  2. Alinity s HBsAg and HBsAg Confirmatory Assay Sensitivity

    Total of 432 specimens from subjects known to be HBsAg positive were tested with Alinity s HBsAg and HBsAg Confirmatory assay.

    Time frame: 10 months

  3. Alinity s HTLV I/II Assay Specificity

    Total of 15877 serum and plasma specimens from whole blood donors specimens were tested with Alinity s HTLV I/II Assay. Repeatedly reactive specimens were further tested with Alinity s HTLV I/II and supplemental assays, if required.

    Time frame: 10 months

  4. Alinity s HTLV I/II Assay Sensitivity

    Total of 706 specimens from subjects known to be HTLV positive were tested with Alinity s HTLV I/II assay. The population consists of the following: Anti-HTLV I Positive n = 461 Anti-HTLV II positive n = 141 Anti-HTLV III positive - Undifferentiated n = 4 Individual with HTLV I/II Associated Diseases n = 100

    Time frame: 10 months

  5. Alinity s Anti-HCV Assay Specificity

    Total of 16,999 serum and plasma specimens from whole blood donors as well as plasmapheresis specimens were tested with Alinity s Anti-HCV assay. Repeatedly reactive specimens were tested further with supplemental assays, if required.

    Time frame: 10 months

  6. Alinity s Anti-HCV Assay Sensitivity

    Total of 402 specimens from subjects known to be Anti-HCV positive were tested with Alinity s Anti-HCV assay.

    Time frame: 10 months

  7. Alinity s HIV Ag/Ab Combo Assay Specificity

    Total of 16996 serum and plasma specimens from whole blood donors as well as plasmapheresis specimens were tested with Alinity s HIV Ag/Ab Combo Assay. Repeatedly reactive specimens were further tested with supplemental assays, if required.

    Time frame: 10 months

  8. Alinity s HIV Ag/Ab Combo Assay Sensitivity

    Total of 1336 specimens from subjects known to be HIV-1/2 positive were tested with Alinity s HIV Ag/Ab Combo assay.

    Time frame: 10 months

  9. Alinity s Anti-HBc Assay Specificity

    Total of 15877 serum and plasma specimens from whole blood donor specimens were tested with Alinity s Anti-HBc Assay. Repeatedly reactive specimens were further tested with supplemental assays, if required.

    Time frame: 10 months

  10. Alinity s Anti-HBc Assay Sensitivity

    Total of 404 specimens from individuals with hepatitis B infection were tested with investigational Alinity s Anti-HBc assay. The population consists of the following: Acute Hepatitis B infection n = 28 Chronic Hepatitis B infection n = 97 Recovered Hepatitis B infection n = 279

    Time frame: 10 months

  11. Alinity s Chagas Assay Specificity

    Total of 15804 serum and plasma specimens from whole blood donors specimens were tested with Alinity s Chagas Assay. Repeatedly reactive specimens were further tested with Alinity s Chagas and supplemental assays, if required.

    Time frame: 10 months

  12. Alinity s Chagas Assay Sensitivity

    Total of 320 specimens from subjects known to be Chagas positive were tested with investigational Alinity s Chagas assay.

    Time frame: 10 months

Secondary outcomes

  1. Alinity s HBsAg Assay Increased Risk of HBV Infection

    Total of 403 specimens from subjects known to be at increased risk for HBV infection were tested with investigational Alinity s HBsAg assay. Sensitivity and Specificity not applicable.

    Time frame: 10 months

  2. Alinity s HBsAg Assay Recovered HBV Infection

    Total of 51 specimens from subjects classified as recovered HBV infection were tested with investigational Alinity s HBsAg assay. Sensitivity/Specificity calculation for this population not applicable.

    Time frame: 10 months

  3. Alinity s HTLV Assay Increased Risk of HTLV Infection

    Total of 502 specimens from subjects known to be at increased risk for HTLV I/II infection were tested with investigational Alinity s HTLV I/II assay. Sensitivity and Specificity not applicable.

    Time frame: 10 months

  4. Alinity s HTLV I/II Assay Endemics

    Total of 509 specimens from subjects collected from areas known to be endemic for HTLV I/II infection were tested with investigational Alinity s HTLV I/II assay. Sensitivity and Specificity not applicable.

    Time frame: 10 months

  5. Alinity s Anti-HCV Assay Increased Risk for HCV

    Total of 407 specimens from subjects known to be at increased risk for HCV infection were tested with investigational Alinity s Anti-HCV assay.

    Time frame: 10 months

  6. Alinity s HIV Ag/Ab Combo Assay Increased Risk of HIV-1/2

    Total of 605 specimens from subjects known to be at increased risk for HIV-1/2 infection were tested with investigational Alinity s HIV Ag/Ab Combo assay. Sensitivity and Specificity not applicable.

    Time frame: 10 months

  7. Alinity s HIV Ag/Ab Combo Assay Endemics

    Total of 535 specimens from subjects collected from areas known to be endemic for HIV infection were tested with investigational Alinity s HIV Ag/Ab Combo assay.

    Time frame: 10 months

  8. Alinity s Anti-HBc Assay Increased Risk for HBV

    Total of 403 specimens from subjects known to be at increased risk for HBV infection were tested with investigational Alinity s Anti-HBc assay.

    Time frame: 10 months

  9. Alinity s Chagas Assay Endemics

    Total of 615 specimens from subjects collected from areas known to be endemic for Chagas infection were tested with investigational Alinity s Chagas assay

    Time frame: 10 months

06

Results

Posted Oct 9, 2019

Participant flow

Alinity s HBsAg: 17987 Alinity s HTLV I/II: 17685 Alinity s Anti-HCV: 17904 Alinity s HIV Ag/Ab Combo: 19571 Alinity s Anti-HBc: 16776 Alinity s Chagas: 16958

Alinity s HBsAg
Participant flow — Alinity s HBsAg
MilestoneSpecificitySensitivityIncreased Risk PopulationEndemic PopulationHBV Recovered Population
Started17095438403051
Completed16993432403051
Not completed1026000
Withdrew: Protocol violation110000
Withdrew: No result on comparator assay170000
Withdrew: No results generated746000
Alinity s HBsAg Confirmatory
Participant flow — Alinity s HBsAg Confirmatory
MilestoneSpecificitySensitivityIncreased Risk PopulationEndemic PopulationHBV Recovered Population
Started8432300
Completed8432300
Not completed00000
Alinity s HBsAg Follow-Up
Participant flow — Alinity s HBsAg Follow-Up
MilestoneSpecificitySensitivityIncreased Risk PopulationEndemic PopulationHBV Recovered Population
Started00000
Completed00000
Not completed00000
Alinity s HTLV I/II
Participant flow — Alinity s HTLV I/II
MilestoneSpecificitySensitivityIncreased Risk PopulationEndemic PopulationHBV Recovered Population
Started159687065025090
Completed158777065025090
Not completed910000
Withdrew: Protocol violation60000
Withdrew: No result on comparator assay150000
Withdrew: No results generated700000
Alinity s HTLV I/II Follow-Up
Participant flow — Alinity s HTLV I/II Follow-Up
MilestoneSpecificitySensitivityIncreased Risk PopulationEndemic PopulationHBV Recovered Population
Started120000
Completed80000
Not completed40000
Withdrew: Lost to follow-up40000
Alinity s Anti-HCV
Participant flow — Alinity s Anti-HCV
MilestoneSpecificitySensitivityIncreased Risk PopulationEndemic PopulationHBV Recovered Population
Started1709540240700
Completed1699940240700
Not completed960000
Withdrew: Protocol violation120000
Withdrew: No results on comparator assay130000
Withdrew: No results generated710000
Alinity s Anti-HCV Follow-Up
Participant flow — Alinity s Anti-HCV Follow-Up
MilestoneSpecificitySensitivityIncreased Risk PopulationEndemic PopulationHBV Recovered Population
Started200000
Completed20000
Not completed180000
Withdrew: Lost to follow-up180000
Alinity s HIV Ag/Ab Combo
Participant flow — Alinity s HIV Ag/Ab Combo
MilestoneSpecificitySensitivityIncreased Risk PopulationEndemic PopulationHBV Recovered Population
Started1709513366055350
Completed1699613366055350
Not completed990000
Withdrew: Protocol violation110000
Withdrew: No results on comparator assay160000
Withdrew: No results generated720000
Alinity s HIV Ag/Ab Combo Follow-Up
Participant flow — Alinity s HIV Ag/Ab Combo Follow-Up
MilestoneSpecificitySensitivityIncreased Risk PopulationEndemic PopulationHBV Recovered Population
Started150000
Completed40000
Not completed110000
Withdrew: Lost to follow-up110000
Alinity s Anti-HBc
Participant flow — Alinity s Anti-HBc
MilestoneSpecificitySensitivityIncreased Risk PopulationEndemic PopulationHBV Recovered Population
Started1596840540300
Completed1587740440300
Not completed911000
Withdrew: Protocol violation80000
Withdrew: No results on comparator assay131000
Withdrew: No results generated700000
Alinity s Anti-HBc Follow-Up
Participant flow — Alinity s Anti-HBc Follow-Up
MilestoneSpecificitySensitivityIncreased Risk PopulationEndemic PopulationHBV Recovered Population
Started160000
Completed20000
Not completed140000
Withdrew: Lost to follow-up140000
Alinity s Chagas
Participant flow — Alinity s Chagas
MilestoneSpecificitySensitivityIncreased Risk PopulationEndemic PopulationHBV Recovered Population
Started1602332006150
Completed1580432006150
Not completed2190000
Withdrew: Protocol violation760000
Withdrew: No results on comparator assay380000
Withdrew: No results generated1050000
Alinity s Chagas Follow-Up
Participant flow — Alinity s Chagas Follow-Up
MilestoneSpecificitySensitivityIncreased Risk PopulationEndemic PopulationHBV Recovered Population
Started140000
Completed10000
Not completed130000
Withdrew: Lost to follow-up130000

Outcome measures

PrimaryAlinity s HBsAg Assay Specificity

Total of 16993 serum and plasma specimens from whole blood donors as well as plasmapheresis specimens were tested with Alinity s HBsAg Assay. Repeatedly reactive specimens were further tested with Alinity s HBsAg Confirmatory and supplemental assays, if required.

Time frame:
10 months
Reported as:
Count of participants · Participants
Alinity s HBsAg Assay Specificity
ParticipantsAlinity s HBsAg Assay Specificity
Alinity s HBsAg Nonreactive HBsAg Negative16985
Alinity s HBsAg Repeatedly Reactive HBsAg Negative6
Alinity s HBsAg Positive2
Statistical analysis
  • Alinity s HBsAg Assay Specificity · Binomial Distribution · Clinical specificity (%): 99.96 · 95% CI 99.92 to 99.99Specificity sample size is a minimum of 15,000 donors.
PrimaryAlinity s HBsAg and HBsAg Confirmatory Assay Sensitivity

Total of 432 specimens from subjects known to be HBsAg positive were tested with Alinity s HBsAg and HBsAg Confirmatory assay.

Time frame:
10 months
Reported as:
Count of participants · Participants
Alinity s HBsAg and HBsAg Confirmatory Assay Sensitivity
ParticipantsAlinity s HBsAg and HBsAg Confirmatory Assay Sensitivity
Alinity s HBsAg Nonreactive0
Alinity s HBsAg Reactive Confirmed432
Alinity s HBsAg Reactive Not Confirmed0
Statistical analysis
  • Alinity s HBsAg and HBsAg Confirmatory Assay Sensitivity · Sensitivity · Point estimate: 100.00 · 95% CI 99.15 to 100.00
PrimaryAlinity s HTLV I/II Assay Specificity

Total of 15877 serum and plasma specimens from whole blood donors specimens were tested with Alinity s HTLV I/II Assay. Repeatedly reactive specimens were further tested with Alinity s HTLV I/II and supplemental assays, if required.

Time frame:
10 months
Reported as:
Count of participants · Participants
Alinity s HTLV I/II Assay Specificity
ParticipantsAlinity s HTLV I/II Assay Specificity
Alinity s HTLV Nonreactive HTLV Neg15864
Alinity s HTLV Repeatedly Reactive HTLV Neg2
HTLV Positive or Indeterminate11
Statistical analysis
  • Alinity s HTLV I/II Assay Specificity · Binomial Distribution · Clinical specificity (%): 99.99 · 95% CI 99.95 to 100.00
PrimaryAlinity s HTLV I/II Assay Sensitivity

Total of 706 specimens from subjects known to be HTLV positive were tested with Alinity s HTLV I/II assay. The population consists of the following: Anti-HTLV I Positive n = 461 Anti-HTLV II positive n = 141 Anti-HTLV III positive - Undifferentiated n = 4 Individual with HTLV I/II Associated Diseases n = 100

Time frame:
10 months
Reported as:
Count of participants · Participants
Alinity s HTLV I/II Assay Sensitivity
ParticipantsAlinity s HTLV I/II Assay Sensitivity
Alinity s HTLV I/II Nonreactive0
Alinity s HTLV I/II Repeatedly Reactive706
Statistical analysis
  • Alinity s HTLV I/II Assay Sensitivity · Sensitivity · Point estimate: 100.00 · 95% CI 99.48 to 100.00
PrimaryAlinity s Anti-HCV Assay Specificity

Total of 16,999 serum and plasma specimens from whole blood donors as well as plasmapheresis specimens were tested with Alinity s Anti-HCV assay. Repeatedly reactive specimens were tested further with supplemental assays, if required.

Time frame:
10 months
Reported as:
Count of participants · Participants
Alinity s Anti-HCV Assay Specificity
ParticipantsAlinity s Anti-HCV Assay Specificity
Alinity s Anti-HCV Nonreactive HCV Negative16975
Alinity s Anti-HCV Repeatedly Reactive HCV Neg14
HCV Positive or Indeterminate10
Statistical analysis
  • Alinity s Anti-HCV Assay Specificity · Binomial Distribution · Clinical specificity (%): 99.92 · 95% CI 99.86 to 99.95
PrimaryAlinity s Anti-HCV Assay Sensitivity

Total of 402 specimens from subjects known to be Anti-HCV positive were tested with Alinity s Anti-HCV assay.

Time frame:
10 months
Reported as:
Count of participants · Participants
Alinity s Anti-HCV Assay Sensitivity
ParticipantsAlinity s Anti-HCV Assay Sensitivity
Alinity s Anti-HCV Nonreactive0
Alinity s Anti-HCV Repeatedly Reactive402
Statistical analysis
  • Alinity s Anti-HCV Assay Sensitivity · Sensitivity · Point estimate: 100.00 · 95% CI 99.09 to 100.00
PrimaryAlinity s HIV Ag/Ab Combo Assay Specificity

Total of 16996 serum and plasma specimens from whole blood donors as well as plasmapheresis specimens were tested with Alinity s HIV Ag/Ab Combo Assay. Repeatedly reactive specimens were further tested with supplemental assays, if required.

Time frame:
10 months
Reported as:
Count of participants · Participants
Alinity s HIV Ag/Ab Combo Assay Specificity
ParticipantsAlinity s HIV Ag/Ab Combo Assay Specificity
Alinity s HIV Nonreactive HIV Negative16981
Alinity s HIV Repeatedly Reactive HIV Negative13
HIV Indeterminate2
Statistical analysis
  • Alinity s HIV Ag/Ab Combo Assay Specificity · Binomial Exact · Clinical specificity (%): 99.92 · 95% CI 99.87 to 99.96
PrimaryAlinity s HIV Ag/Ab Combo Assay Sensitivity

Total of 1336 specimens from subjects known to be HIV-1/2 positive were tested with Alinity s HIV Ag/Ab Combo assay.

Time frame:
10 months
Reported as:
Count of participants · Participants
Alinity s HIV Ag/Ab Combo Assay Sensitivity
ParticipantsAlinity s HIV Ag/Ab Combo Assay Sensitivity
Alinity s HIV Ag/Ab Combo Nonreactive0
Alinity s HIV Ag/Ab Combo Repeatedly Reactive Pos1336
Alinity s HIV Ag/Ab Combo Repeatedly Reactive Neg0
Statistical analysis
  • Alinity s HIV Ag/Ab Combo Assay Sensitivity · Sensitivity · Point estimate: 100.00 · 95% CI 99.72 to 100.00
PrimaryAlinity s Anti-HBc Assay Specificity

Total of 15877 serum and plasma specimens from whole blood donor specimens were tested with Alinity s Anti-HBc Assay. Repeatedly reactive specimens were further tested with supplemental assays, if required.

Time frame:
10 months
Reported as:
Count of participants · Participants
Alinity s Anti-HBc Assay Specificity
ParticipantsAlinity s Anti-HBc Assay Specificity
Alinity s Anti-HBc Nonreactive Anti-HBc Negative15833
Alinity s Anti-HBc Repeatedly Reactive AntiHBc Neg16
Anti-HBc Positive28
Statistical analysis
  • Alinity s Anti-HBc Assay Specificity · Binomial Exact · Clinical specificity (%): 99.90 · 95% CI 99.84 to 99.94
PrimaryAlinity s Anti-HBc Assay Sensitivity

Total of 404 specimens from individuals with hepatitis B infection were tested with investigational Alinity s Anti-HBc assay. The population consists of the following: Acute Hepatitis B infection n = 28 Chronic Hepatitis B infection n = 97 Recovered Hepatitis B infection n = 279

Time frame:
10 months
Reported as:
Count of participants · Participants
Alinity s Anti-HBc Assay Sensitivity
ParticipantsAlinity s Anti-HBc Assay Sensitivity
Alinity s Anti-HBc Nonreactive0
Alinity s Anti-HBc Repeatedly Reactive Positive404
Alinity s Anti-HBc Repeatedly Reactive Negative0
Statistical analysis
  • Alinity s Anti-HBc Assay Sensitivity · Sensitivity · Point estimate: 100.00 · 95% CI 99.09 to 100.00
PrimaryAlinity s Chagas Assay Specificity

Total of 15804 serum and plasma specimens from whole blood donors specimens were tested with Alinity s Chagas Assay. Repeatedly reactive specimens were further tested with Alinity s Chagas and supplemental assays, if required.

Time frame:
10 months
Reported as:
Count of participants · Participants
Alinity s Chagas Assay Specificity
ParticipantsAlinity s Chagas Assay Specificity
Alinity s Chagas Nonreactive Chagas Negative15787
Alinity s Chagas Repeatedly Reactive Chagas Neg3
Chagas Positive or Indeterminate14
Statistical analysis
  • Alinity s Chagas Assay Specificity · Binomial Exact · Clinical specificity (%): 99.98 · 95% CI 99.94 to 100.00
PrimaryAlinity s Chagas Assay Sensitivity

Total of 320 specimens from subjects known to be Chagas positive were tested with investigational Alinity s Chagas assay.

Time frame:
10 months
Reported as:
Count of participants · Participants
Alinity s Chagas Assay Sensitivity
ParticipantsAlinity s Chagas Assay Sensitivity
Alinity s Chagas Nonreactive0
Alinity s Chagas Repeatedly Reactive Positive320
Alinity s Chagas Repeatedly Reactive Negative0
Statistical analysis
  • Alinity s Chagas Assay Sensitivity · Sensitivity · Point estimate: 100.00 · 95% CI 98.85 to 100.00
SecondaryAlinity s HBsAg Assay Increased Risk of HBV Infection

Total of 403 specimens from subjects known to be at increased risk for HBV infection were tested with investigational Alinity s HBsAg assay. Sensitivity and Specificity not applicable.

Time frame:
10 months
Reported as:
Count of participants · Participants
Alinity s HBsAg Assay Increased Risk of HBV Infection
ParticipantsAlinity s HBsAg Assay Increased Risk of HBV Infection
Alinity s HBsAg Nonreactive Negative400
Alinity s HBsAg Nonreactive Positive0
Alinity s HBsAg Reactive Confirmed3
Alinity s HBsAg Reactive Not Confirmed0
SecondaryAlinity s HBsAg Assay Recovered HBV Infection

Total of 51 specimens from subjects classified as recovered HBV infection were tested with investigational Alinity s HBsAg assay. Sensitivity/Specificity calculation for this population not applicable.

Time frame:
10 months
Reported as:
Count of participants · Participants
Alinity s HBsAg Assay Recovered HBV Infection
ParticipantsAlinity s HBsAg Assay Recovered HBV Infection
Alinity s HBsAg Nonreactive Negative51
Alinity s HBsAg Nonreactive Positive0
Alinity s HBsAg Reactive Confirmed0
Alinity s HBsAg Reactive Not Confirmed0
SecondaryAlinity s HTLV Assay Increased Risk of HTLV Infection

Total of 502 specimens from subjects known to be at increased risk for HTLV I/II infection were tested with investigational Alinity s HTLV I/II assay. Sensitivity and Specificity not applicable.

Time frame:
10 months
Reported as:
Count of participants · Participants
Alinity s HTLV Assay Increased Risk of HTLV Infection
ParticipantsAlinity s HTLV Assay Increased Risk of HTLV-I/II Infection
Alinity s HTLV I/II Nonreactive Negative491
Alinity s HTLV I/II Nonreactive Positive0
Alinity s HTLV I/II Nonreactive Indeterminate0
Alinity s HTLV I/II Reactive Indeterminate0
Alinity s HTLV I/II Reactive Positive11
Alinity s HTLV I/II Reactive Negative0
SecondaryAlinity s HTLV I/II Assay Endemics

Total of 509 specimens from subjects collected from areas known to be endemic for HTLV I/II infection were tested with investigational Alinity s HTLV I/II assay. Sensitivity and Specificity not applicable.

Time frame:
10 months
Reported as:
Count of participants · Participants
Alinity s HTLV I/II Assay Endemics
ParticipantsAlinity s HTLV I/II Assay Endemics
Alinity s HTLV I/II Nonreactive Negative495
Alinity s HTLV I/II Nonreactive Positive0
Alinity s HTLV I/II Nonreactive Indeterminate4
Alinity s HTLV I/II Reactive Indeterminate0
Alinity s HTLV I/II Reactive Positive10
Alinity s HTLV I/II Reactive Negative0
SecondaryAlinity s Anti-HCV Assay Increased Risk for HCV

Total of 407 specimens from subjects known to be at increased risk for HCV infection were tested with investigational Alinity s Anti-HCV assay.

Time frame:
10 months
Reported as:
Count of participants · Participants
Alinity s Anti-HCV Assay Increased Risk for HCV
ParticipantsAlinity s Anti-HCV Assay Increased Risk for HCV
Alinity s Anti-HCV Nonreactive Negative316
Alinity s Anti-HCV Noreactive Positive0
Alinity s Anti-HCV Nonreactive Indeterminate0
Alinity s Anti-HCV Reactive Indeterminate0
Alinity s Anti-HCV Reactive Positive90
Alinity s Anti-HCV Reactive Negative1
Statistical analysis
  • Alinity s Anti-HCV Assay Increased Risk for HCV · Sensitivity · Point estimate: 100.00 · 95% CI 95.98 to 100.00
SecondaryAlinity s HIV Ag/Ab Combo Assay Increased Risk of HIV-1/2

Total of 605 specimens from subjects known to be at increased risk for HIV-1/2 infection were tested with investigational Alinity s HIV Ag/Ab Combo assay. Sensitivity and Specificity not applicable.

Time frame:
10 months
Reported as:
Count of participants · Participants
Alinity s HIV Ag/Ab Combo Assay Increased Risk of HIV-1/2
ParticipantsAlinity s HIV Ag/Ab Combo Assay Increased Risk for HIV-1/2
Alinity s HIV Ag/Ab Combo Nonreactive Negative582
Alinity s HIV Ag/Ab Combo Nonreactive Positive0
Alinity s HIV Ag/Ab Comb Nonreactive Indeterminate0
Alinity s HIV Ag/Ab Combo Reactive Indeterminate0
Alinity s HIV Ag/Ab Combo Reactive Positive21
Alinity s HIV Ag/Ab Combo Reactive Negative2
SecondaryAlinity s HIV Ag/Ab Combo Assay Endemics

Total of 535 specimens from subjects collected from areas known to be endemic for HIV infection were tested with investigational Alinity s HIV Ag/Ab Combo assay.

Time frame:
10 months
Reported as:
Count of participants · Participants
Alinity s HIV Ag/Ab Combo Assay Endemics
ParticipantsAlinity s HIV Ag/Ab Combo Assay Endemics
Alinity s HIV Ag/Ab Combo Nonreactive Negative472
Alinity s HIV Ag/Ab Combo Nonreactive Positive0
Alinity s HIV Ag/Ab Comb Nonreactive Indeterminate2
Alinity s HIV Ag/Ab Combo Reactive Indeterminate10
Alinity s HIV Ag/Ab Combo Reactive Positive49
Alinity s HIV Ag/Ab Combo Reactive Negative2
Statistical analysis
  • Alinity s HIV Ag/Ab Combo Assay Endemics · Sensitivity · Point estimate: 100.00 · 95% CI 92.75 to 100.00
SecondaryAlinity s Anti-HBc Assay Increased Risk for HBV

Total of 403 specimens from subjects known to be at increased risk for HBV infection were tested with investigational Alinity s Anti-HBc assay.

Time frame:
10 months
Reported as:
Count of participants · Participants
Alinity s Anti-HBc Assay Increased Risk for HBV
ParticipantsAlinity s Anti-HBc Assay Increased Risk for HBV
Alinity s Anti-HBc Nonreactive Negative319
Alinity s Anti-HBc Nonreactive Positive0
Alinity s Anti-HBc Reactive Positive70
Alinity s Anti-HBc Reactive Negative14
Statistical analysis
  • Alinity s Anti-HBc Assay Increased Risk for HBV · Sensitivity · Point estimate: 100.00 · 95% CI 94.87 to 100.00
SecondaryAlinity s Chagas Assay Endemics

Total of 615 specimens from subjects collected from areas known to be endemic for Chagas infection were tested with investigational Alinity s Chagas assay

Time frame:
10 months
Reported as:
Count of participants · Participants
Alinity s Chagas Assay Endemics
ParticipantsAlinity s Chagas Assay Endemics
Alinity s Chagas Nonreactive Negative466
Alinity s Chagas Noreactive Positive2
Alinity s Chagas Nonreactive Indeterminate0
Alinity s Chagas Reactive Indeterminate0
Alinity s Chagas Reactive Positive146
Alinity s Chagas Reactive Negative1
Statistical analysis
  • Alinity s Chagas Assay Endemics · Sensitivity · Point estimate: 98.65 · 95% CI 95.20 to 99.84

Adverse events

Collected over Subject adverse events related to blood collection during the 3-6 months of donor follow-up specimen collection.. Non-serious events are listed at a 0% frequency threshold.

Adverse event summary by group
GroupDeathsSeriousOther
Alinity s HBsAg Follow-Up Specimen Collection———
Alinity s HTLV I/II Follow-Up Specimen Collection0/8 (0%)0/8 (0%)0/8 (0%)
Alinity s Anti-HCV Follow-Up Specimen Collection0/2 (0%)0/2 (0%)0/2 (0%)
Alinity s HIV Ag/Ab Combo Follow-Up Specimen Collection0/4 (0%)0/4 (0%)0/4 (0%)
Alinity s Anti-HBc Follow-Up Specimen Collection0/2 (0%)0/2 (0%)0/2 (0%)
Alinity s Chagas Follow-Up Specimen Collection0/1 (0%)0/1 (0%)0/1 (0%)

Baseline characteristics

Donors and pedigree

Age, Customized
Age, Customized(Participants)SpecificitySensitivityIncreased Risk PopulationEndemic PopulationHBV Recovered PopulationTotal
≥16 years — Alinity s HBsAg1699343240305117879
≥16 years — Alinity s HTLV I/II15877706502509017594
≥16 years — Alinity s Anti-HCV169994024070017808
≥16 years — Alinity s HIV Ag/Ab Combo169961336605535019472
≥16 years — Alinity s Anti-HBc158774044030016684
≥16 years — Alinity s Chagas158043200615016739
Sex/Gender, Customized
Sex/Gender, Customized(Participants)SpecificitySensitivityIncreased Risk PopulationEndemic PopulationHBV Recovered PopulationTotal
Alinity s HBsAg Gender — Male0312288023623
Alinity s HBsAg Gender — Female092115028235
Alinity s HBsAg Gender — Unknown169932800017021
Alinity s HTLV I/II Gender — Male01823353160833
Alinity s HTLV I/II Gender — Female02901671930650
Alinity s HTLV I/II Gender — Unknown1587723400016111
Alinity s Anti-HCV Gender — Male024129100532
Alinity s Anti-HCV Gender — Female016111600277
Alinity s Anti-HCV Gender — Unknown16999000016999
Alinity s HIV Ag/Ab Combo Gender — Male089142027901590
Alinity s HIV Ag/Ab Combo Gender — Female02221852560663
Alinity s HIV Ag/Ab Combo Gender — Unknown1699622300017219
Alinity s Anti-HBc Gender — Male023028800518
Alinity s Anti-HBc Gender — Female016511500280
Alinity s Anti-HBc Gender — Unknown15877900015886
Alinity s Chagas Gender — Male010402160320
Alinity s Chagas Gender — Female010203990501
Alinity s Chagas Gender — Unknown1580411400015918
Race/Ethnicity, Customized
Race/Ethnicity, Customized(Participants)SpecificitySensitivityIncreased Risk PopulationEndemic PopulationHBV Recovered PopulationTotal
Alinity s HBsAg Race/Ethnicity — Asian02430128
Alinity s HBsAg Race/Ethnicity — Black or African American012324406373
Alinity s HBsAg Race/Ethnicity — White04579013137
Alinity s HBsAg Race/Ethnicity — Hispanic or Latino0337102106
Alinity s HBsAg Race/Ethnicity — American Indian or Alaskan Native003003
Alinity s HBsAg Race/Ethnicity — Other02330228
Alinity s HBsAg Race/Ethnicity — Unknown16993184002717204
Alinity s HTLV I/II Race/Ethnicity — Asian02640030
Alinity s HTLV I/II Race/Ethnicity — Black or African American0631543050522
Alinity s HTLV I/II Race/Ethnicity — White011425600370
Alinity s HTLV I/II Race/Ethnicity — Hispanic or Latino0224872040515
Alinity s HTLV I/II Race/Ethnicity — American Indian or Alaskan Native051006
Alinity s HTLV I/II Race/Ethnicity — Other070007
Alinity s HTLV I/II Race/Ethnicity — Unknown1587726700016144
Alinity s Anti-HCV Race/Ethnicity — Asian002002
Alinity s Anti-HCV Race/Ethnicity — Black or African American09523900334
Alinity s Anti-HCV Race/Ethnicity — White01410000114
Alinity s Anti-HCV Race/Ethnicity — Hispanic or Latino012620074
Alinity s Anti-HCV Race/Ethnicity — American Indian or Alaskan Native001001
Alinity s Anti-HCV Race/Ethnicity — Other001001
Alinity s Anti-HCV Race/Ethnicity — Unknown1699928120017282
Alinity s HIV Ag/Ab Combo Race/Ethnicity — Asian045009
Alinity s HIV Ag/Ab Combo Race/Ethnicity — Black or African American036440453501303
Alinity s HIV Ag/Ab Combo Race/Ethnicity — White028611500401
Alinity s HIV Ag/Ab Combo Race/Ethnicity — Hispanic or Latino04308100511
Alinity s HIV Ag/Ab Combo Race/Ethnicity — American Indian or Alaskan Native050005
Alinity s HIV Ag/Ab Combo Race/Ethnicity — Other040004
Alinity s HIV Ag/Ab Combo Race/Ethnicity — Unknown1699624300017239
Alinity s Anti-HBc Race/Ethnicity — Asian053008
Alinity s Anti-HBc Race/Ethnicity — Black or African American04024400284
Alinity s Anti-HBc Race/Ethnicity — White0397900118
Alinity s Anti-HBc Race/Ethnicity — Hispanic or Latino04710075
Alinity s Anti-HBc Race/Ethnicity — American Indian or Alaskan Native013004
Alinity s Anti-HBc Race/Ethnicity — Other01830021
Alinity s Anti-HBc Race/Ethnicity — Unknown1587729700016174
Alinity s Chagas Race/Ethnicity — Asian000000
Alinity s Chagas Race/Ethnicity — Black or African American000000
Alinity s Chagas Race/Ethnicity — White0109010
Alinity s Chagas Race/Ethnicity — Hispanic or Latino020505760781
Alinity s Chagas Race/Ethnicity — American Indian or Alaskan Native00030030
Alinity s Chagas Race/Ethnicity — Other000000
Alinity s Chagas Race/Ethnicity — Unknown1580411400015918
Region of Enrollment
Region of Enrollment(Participants)SpecificitySensitivityIncreased Risk PopulationEndemic PopulationHBV Recovered PopulationTotal
United States — Alinity s HBsAg1699304030017396
United States — Alinity s HTLV I/II15877000015877
United States — Alinity s Anti-HCV1699904070017406
United States — Alinity s HIV Ag/Ab Combo1699606050017601
United States — Alinity s Anti-HBc1587704030016280
United States — Alinity s Chagas1580411400015918
United States — Not collected032805020513833
Haiti — Alinity s HBsAg000000
Haiti — Alinity s HTLV I/II0002040204
Haiti — Alinity s Anti-HCV000000
Haiti — Alinity s HIV Ag/Ab Combo000000
Haiti — Alinity s Anti-HBc000000
Haiti — Alinity s Chagas000000
Haiti — Not collected000000
Africa — Alinity s HBsAg000000
Africa — Alinity s HTLV I/II0001000100
Africa — Alinity s Anti-HCV000000
Africa — Alinity s HIV Ag/Ab Combo0005350535
Africa — Alinity s Anti-HBc000000
Africa — Alinity s Chagas000000
Africa — Not collected000000
Mexico — Alinity s HBsAg000000
Mexico — Alinity s HTLV I/II000000
Mexico — Alinity s Anti-HCV000000
Mexico — Alinity s HIV Ag/Ab Combo000000
Mexico — Alinity s Anti-HBc000000
Mexico — Alinity s Chagas0901000109
Mexico — Not collected000000
South America — Alinity s HBsAg000000
South America — Alinity s HTLV I/II0002050205
South America — Alinity s Anti-HCV000000
South America — Alinity s HIV Ag/Ab Combo000000
South America — Alinity s Anti-HBc000000
South America — Alinity s Chagas019703160513
South America — Not collected000000
Central America — Alinity s HBsAg000000
Central America — Alinity s HTLV I/II000000
Central America — Alinity s Anti-HCV000000
Central America — Alinity s HIV Ag/Ab Combo000000
Central America — Alinity s Anti-HBc000000
Central America — Alinity s Chagas0001990199
Central America — Not collected000000
07

Study locations

5 sites
  • Biotest Pharmaceuticals Corporation
    Boca Raton, Florida 33431, United States
  • American Red Cross National Testing Laboratory
    Saint Louis, Missouri 63108, United States
  • CSL Plasma Inc.
    Knoxville, Tennessee 37932, United States
  • QualTex Laboratories
    San Antonio, Texas 78201, United States
  • Bloodworks Northwest
    Renton, Washington 98057, United States
08

References and documents

Study documents

  • Informed consent form · Aug 17, 2017
  • Informed consent form · Nov 17, 2017
  • Protocol and statistical analysis plan · Apr 24, 2018
  • Informed consent form · Aug 23, 2017
  • Informed consent form · Nov 17, 2017

Documents are hosted by the registry — open the source record to download them.

Individual participant data

Plan to share: No

09

Registry details

Key details

Study ID
NCT03285295
Lead sponsor
Abbott Diagnostics Division
Responsible party
Sponsor
First posted
Sep 18, 2017
Start date
Sep 15, 2017
Primary completion
May 23, 2018
Completion
Jun 21, 2018
Results posted
Oct 9, 2019
Last update
Oct 9, 2019

Study contacts

Nancy R Haley, M.D.
principal investigator · Bloodworks Northwest
Thomas S Jones, Ph.D.
principal investigator · QualTex Laboratories
Susan S Ganz, M.D.
principal investigator · Biotest Pharmaceuticals Corporation
Toby L Simon, M.D.
principal investigator · CSL Plasma Inc.
Susan L Stramer, Ph.D.
principal investigator · American Red Cross National Testing Laboratory

Oversight

Data monitoring committee
No
FDA-regulated drug
No
FDA-regulated device
Yes
View the source record on ClinicalTrials.gov ↗

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