An observational study in Tooth, Impacted, sponsored by Central Hospital, Nancy, France. Status unknown. Open to participants aged 13 Years to 17 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2017-06-23.
Sponsored by Central Hospital, Nancy, France · Observational
The main limitation in bone regeneration is the lack of vascularization of the newly shaped tissue. The main objective of this project is to check if the simultaneous differentiation of dental mesenchymal stem cells toward osteoblastic and endothelial lineage permits to obtain a new pre-vascularized tissue engineered bone construct.
We will then evaluate the boosting effect of the conditionned medium on cell differentiation and production of a prevascularized bone construct.
This project aims to develop a new pre-vascularized tissue engineered bone construct, using human cells of a simple and non invasive tissue source: dental pulp.
We will isolate mesenchymal stem cells from dental tissue of wisdom teeth, extracted from patients aged between 13 and 17 years old.
The objectives are: (i) to assess in combination the endothelial differentiation and osteoblastic differentiation of dental pulp stem cells (immature third molar); (ii) to study the boosting effect of the conditionned medium on cell differentiation and production of a prevascularized bone construct.
The differentiation will be monitored by evaluation of biosynthesized matrix, bone mineralization (immunohistochimie, Westen Blot), histological response (Von Kossa, Alizarin Red) and presence and quantification of pseudo-vascular structures (angiogenesis assay), compared to reference value (commercial differentiation medium).
200 studies on the registry are indexed under Tooth, Impacted; 21 are open to participants now.
This study's planned enrollment of 40 is below the median of 60 across 31 observational studies indexed under Tooth, Impacted.
Browse Tooth, Impacted studies →Central Hospital, Nancy, France is the lead sponsor of 796 studies on the registry; 187 are open to participants now.
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Patients aged between 13 and 17 years, who will be operated for wisdom tooth avulsion
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Exclusion Criteria:
teeth collection
Bone mineralization
Measured parameter is equal or superior to reference value (commercial differentiation medium)
Time frame: Day 28 of cell culture
presence and quantification of pseudo-vascular structures
Measured parameter is equal or superior to reference value (commercial differentiation medium)
Time frame: Day 28 of cell culture
No study locations are listed for this record.
Plan to share: No
No publications or documents are linked to this record.
This study is status unknown, as verified in Jun 2017. You cannot join it, but the record below documents what was studied.
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Central Hospital, Nancy, France →