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CompletedNCT03162367Updated Mar 18, 2020

Efficacy Of Autologous Epidermal Cell Suspension And Silver Sulfadiazine Ointment In Burn

A Phase 2 interventional study of Autologous epidermal cell suspension and Silver sulfadiazine ointment in Burn, sponsored by SCARM Institute, Tabriz, Iran. Completed at 1 site in Iran, Islamic Republic of. Open to participants aged 18 Years to 65 Years. Per ClinicalTrials.gov, last updated 2020-03-18.

Sponsored by SCARM Institute, Tabriz, Iran · Phase 2, Interventional, and Treatment

Phase
Phase 2
Study type
Interventional
Enrollment
40
Allocation
Non-randomized
Ages
18 Years to 65 Years
Sex
All
01

Study summary

Different medical treatments are available for treatment of skin burns such as skin grafts and silver sulfadiazine ointment. These treatments are used for second degree burn as routine technique in burns units. Cell therapy is a new approach for treatment of skin disease.In this study we use autologous epidermal cell suspension to achieve better cosmetic and functional results in treatment of second degree burn.

Read the detailed description

In this study 20 patients in Burn Department of Sina Hospital Tabriz, with at least 2 areas of second degree burn randomly choose. First skin samples are taken from normal skin by 4mm punch and send to the laboratory for preparation of epidermal cell suspension and this suspension is transferred to the one area of second degree burn in each patient and the other area in the same patient dresses by silver sulfadiazine ointment. The dressing changes are followed up for 12h for first assessment of therapy until 10th day after intervention. In the days of 5, 10, 15 and 20 the percentage of re-epithelization for each treatment will be measured. After 2 months, patients are visited and any complications check for scar formation, pain, post inflammatory hyper-pigmentation.

02

Conditions studied

  • Burn

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Keywords

  • Burn
  • Epidermal cell suspension
03

Who can participate

Ages eligible
18 Years to 65 Years
Sexes eligible
All
Accepts healthy volunteers
No

Inclusion criteria

  • Healthy patient with second degree burn admitted in burn unit

Exclusion criteria

Exclusion Criteria:

  • Pregnancy-lactation
  • Immunosuppression like receiving chemotherapy or radiotherapy
  • Patient unsatisfied to going on study
04

Study design

Phase
Phase 2
Primary purpose
Treatment
Allocation
Non-randomized
Intervention model
Parallel assignment
Masking
Double (Participant, Outcomes assessor)
Enrollment
40 participants (actual)

Study arms

  • Experimental
    Autologous epidermal cell suspension group

    Biological: Autologous epidermal cell suspension

  • Active comparator
    Silver sulfadiazine ointment group

    Drug: Silver sulfadiazine ointment

Interventions

  • BiologicalAutologous epidermal cell suspension

    First skin samples taken from normal skin by 4mm punch will be send to laboratory for preparation of epidermal cell suspension and this suspension transfer to one area of second degree burn

  • DrugSilver sulfadiazine ointment

    the area with second degree burn dresses by silver sulfadiazine ointment and dressing change each 12 hour till 10 day

05

What researchers measure

Primary outcomes

  1. Change in re-epithelization rate with autologous epidermal cell suspension graft from Baseline

    Measurement the percentage of re-epithelization ( 0-30%=poor; 30-60%=moderate; 60-90%=good; \>90%=complete)

    Time frame: up to 20 days

  2. Change in re-epithelization rate with silver sulfadiazine ointment from Baseline

    Measure percentage of re-epithelization ( 0-30%=poor; 30-60%=moderate; 60-90%=good; \>90%=complete)

    Time frame: up to 20 days

Secondary outcomes

  1. Scar formation

    Observe patient and take photo

    Time frame: After 2 month

  2. Post inflammatory hyperpigmentation

    Observe patient and take photo

    Time frame: After 2 month

06

Study locations

1 site
  • Sina hospital
    Tabriz, Iran, Islamic Republic of
07

References and documents

Publications

  • You HJ, Han SK. Cell therapy for wound healing. J Korean Med Sci. 2014 Mar;29(3):311-9. doi: 10.3346/jkms.2014.29.3.311. Epub 2014 Feb 27. PubMed 24616577 ↗
  • Zhao H, Chen Y, Zhang C, Fu X. Autologous epidermal cell suspension: A promising treatment for chronic wounds. J Tissue Viability. 2016 Feb;25(1):50-6. doi: 10.1016/j.jtv.2015.11.003. Epub 2015 Dec 8. PubMed 26706649 ↗
  • Mcheik JN, Barrault C, Levard G, Morel F, Bernard FX, Lecron JC. Epidermal healing in burns: autologous keratinocyte transplantation as a standard procedure: update and perspective. Plast Reconstr Surg Glob Open. 2014 Oct 7;2(9):e218. doi: 10.1097/GOX.0000000000000176. eCollection 2014 Sep. PubMed 25426401 ↗
  • Gardien KL, Marck RE, Bloemen MC, Waaijman T, Gibbs S, Ulrich MM, Middelkoop E; Dutch Outback Study Group1. Outcome of Burns Treated With Autologous Cultured Proliferating Epidermal Cells: A Prospective Randomized Multicenter Intrapatient Comparative Trial. Cell Transplant. 2016;25(3):437-48. doi: 10.3727/096368915X689569. Epub 2015 Sep 28. PubMed 26419871 ↗

Individual participant data

Plan to share: No

08

Registry details

Key details

Study ID
NCT03162367
Lead sponsor
SCARM Institute, Tabriz, Iran
Responsible party
Sponsor
First posted
May 22, 2017
Start date
Apr 10, 2019
Primary completion
Mar 15, 2020
Completion
Mar 15, 2020
Last update
Mar 18, 2020

Study contacts

Mohamad Reza Ranjkesh, MD, Dermatologist
study director · SCARM institute

Oversight

Data monitoring committee
Yes
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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