An interventional study of Normal and Moderate in Healthy, sponsored by University of Toronto. Completed at 1 site in Canada. Open to male participants aged 18 Years to 50 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2019-03-29.
Sponsored by University of Toronto · Not applicable, Interventional, and Basic science
Protein requirements in individuals who participate in endurance-based exercise training have been suggested to be greater than the current recommended dietary allowance (RDA).
Our recent study using the minimally invasive indicator amino acid oxidation (IAAO) technique have suggested that protein requirements in young men are at least 30% higher than the recommended protein intake.
The present study will investigate the impact of protein sufficiency on protein metabolism and performance during intensified training periods as a means to further our understanding of the nutritional requirements for the endurance athlete.
University of Toronto is the lead sponsor of 397 studies on the registry; 59 are open to participants now.
Counted across the registry records on this site, refreshed daily.
Exclusion Criteria:
participants will receive the controlled-diet containing 0.8 g protein /kg/day and the test drink containing 0.14 g protein/kg/d.
Dietary Supplement: Normal · Other: Controlled-diet
participants will receive the controlled-diet containing 1.20 g protein /kg/day and the test drink containing 0.40 g protein/kg/d.
Dietary Supplement: Moderate · Other: Controlled-diet
participants will receive the controlled-diet containing 1.83 g protein /kg/day and the test drink containing 1.03 g protein/kg/d.
Dietary Supplement: High · Other: Controlled-diet
test drink which contains low amount of amino acids (0.14 g/kg/day)
test drink which contains moderate amount of amino acids (0.40 g/kg/day)
test drink which contains high amount of amino acids (1.03 g/kg/day)
the diet containing 0.8 g protein /kg/day
Change in 24h whole body protein balance
Protein balance (mg/kg/d; protein synthesis - protein breakdown) will be measured using \[15N\]Glycine method on day 1 and day 4 to determine the change over the intensified training period to compare between protein levels
Time frame: Difference between day 1 and day 4 whole body protein balance
Change from baseline physical performance (5 km time trial) 5days after the beginning of training
Before and after 4 days of intensified training period, Duration (seconds) for completing 5-km running on treadmill will be measured.
Time frame: 5days after the beginning of training
change from baseline Physical performance (muscle strength) 5days after the beginning of training
Maximal strength will be assessed by a maximal isometric voluntary contraction (MVC), which will be tested for the knee-extensors using a custom-made knee-extension apparatus after 4 days intensified training.
Time frame: 5days after the beginning of training
change from baseline Physical Performance (muscle power) 5days after the beginning of training
Peak lower body power will be measured by force platform after 4days-intensified training.
Time frame: 5days after the beginning of training
Creatine Phosphate Kinase activity on day 5
Muscle damage markers (blood Creatine Phosphate Kinase activity, IU/L) will be compared between the experimental arms 5days after the beginning of training
Time frame: 5days after the beginning of training
Myoglobin concentration on day 5
Muscle damage markers (blood Mb Concentration, mg/mL) will be compared between the experimental arms 5days after the beginning of training
Time frame: 5days after the beginning of training
IL-6 concentration on day 5
inflammatory markers (IL-6 concentration, pg/ml) will be compared between the experimental arms 5days after the beginning of training
Time frame: 5days after the beginning of training
CRP concentration on day 5
inflammatory markers (CRP concentration, mg/L) will be compared between the experimental arms 5days after the beginning of training
Time frame: 5days after the beginning of training
amino acid concentration on day 5
amino acid concentration (mmol/mL) will be compared between the experimental arms 5days after the beginning of training
Time frame: 5days after the beginning of training
Plan to share: No
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This study is completed, as verified in Mar 2019. You cannot join it, but the record below documents what was studied.
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