An interventional study of High-intensity interval training and Moderate training in Healthy, sponsored by Norwegian University of Science and Technology. Terminated at 1 site in Norway. Open to male participants aged 70 Years to 75 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2019-05-20.
Sponsored by Norwegian University of Science and Technology · Not applicable, Interventional, and Basic science
The purpose of the study was to investigate whether regular moderate- or high-intensity interval training can change the DNA methylation in a long-lasting state, and whether these changes affect the gene expression. In addition, the investigators will determine whether the intensity of the training could be an affecting factor of the possible DNA-methylation change as well. The null hypothesis was that no such exercise-induced changes occur.
Norwegian University of Science and Technology is the lead sponsor of 487 studies on the registry; 52 are open to participants now.
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The High-intensity interval training group was instructed to complete exercise sessions of 4x4-minutes intervals at 85-95% of maximal heart rate, 3 times a week for 8 weeks. This type of exercise include 10 minutes warm-up, followed by four intervals of four minutes high-intensity (85-95% of HRmax), with three minutes active breaks (70% of HRmax) between each interval, and ending with seven minutes cool-down. An exercise session last for 42 minutes, where the participants should breathe heavily and feel exhausted four times.
Behavioral: High-intensity interval training
The moderate training group was instructed to complete exercise sessions of 50 minutes at 70% of maximal heart rate, 3 times a week for 8 weeks.
Behavioral: Moderate training
The control group followed the baseline and follow-up tests, and was asked to live normally and not change their dietary pattern or exercise habits during their participation in this project. Based on the well-known beneficial effects of exercise on general health, it was regarded unethical to demand the control group to desist from any types of physical activity during the project period. They got no further follow-up on exercise.
Interval training 3 times a week for 8 weeks
Moderate training 3 times a week for 8 weeks
DNA-methylation
From the skeletal muscle biopsies RNA and DNA was isolated, DNA was further bi-sulfite converted and DNA-methylation was analyzed using the Illumina HumanMethylation450 BeadChip. The RNA was further used for gene expression analyses by real-time RTqPCR.
Time frame: 8 weeks
Gene Expression
Time frame: 8 weeks
Maximal Aerobic Capacity
measured on treadmill at baseline and after eight weeks, also biopsies from Vastus lateralis were sampled.
Time frame: 8 weeks
Plan to share: No
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This study is terminated, as verified in May 2019. You cannot join it, but the record below documents what was studied.
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Norwegian University of Science and Technology