An interventional study of 20-Meter Shuttle Run Test and Wingate Anaerobic Test in Sedentary Behavior, Acute Exercise and Endocrine Response, sponsored by Assoc. Prof. Dr. Özgür EKEN. Recruiting at 1 site in Turkey (Türkiye). Open to female participants aged 18 Years to 24 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2026-10-27.
Sponsored by Assoc. Prof. Dr. Özgür EKEN · Not applicable, Interventional, and Basic science
This study aims to compare acute thyroid hormone and cortisol responses to aerobic and anaerobic exercise in sedentary women. Thirty sedentary women aged 18-25 years will voluntarily participate in the study. Participants will complete a 20-meter shuttle run test as the aerobic exercise protocol and a 30-second Wingate test as the anaerobic exercise protocol in separate testing sessions. Blood samples will be collected immediately before and after each exercise protocol. Serum thyroid-stimulating hormone (TSH), triiodothyronine (T3), thyroxine (T4), and cortisol concentrations will be determined using chemiluminescence-based laboratory methods. Changes in hormone concentrations will be compared between the aerobic and anaerobic exercise conditions.
This interventional study is designed to compare the acute endocrine responses elicited by aerobic and anaerobic exercise in sedentary women. A total of 30 healthy sedentary women aged 18-25 years will voluntarily participate. Individuals who have engaged in regular exercise or sports training, experienced a serious upper- or lower-extremity injury during the previous six months, or had a neurological, auditory, visual, or vestibular disorder during the previous year will not be included. Before participation, all individuals will be informed about the study procedures, provide written informed consent, and undergo medical screening.
Each participant will complete aerobic and anaerobic exercise protocols in separate testing sessions. The aerobic exercise protocol will consist of a progressive 20-meter shuttle run test. Participants will run continuously between two lines positioned 20 meters apart, following prerecorded audio signals. Running speed will progressively increase as the interval between signals decreases. The test will end when a participant fails to reach the designated line before the signal on two consecutive occasions.
The anaerobic exercise protocol will consist of a 30-second Wingate test performed on a Monark cycle ergometer connected to computerized analysis software. Following an unloaded acceleration period of approximately 3-4 seconds, an external resistance equivalent to 65 g/kg of body mass will be applied. Participants will be instructed to pedal against this resistance at maximal effort for 30 seconds.
Four venous blood samples will be collected from each participant: immediately before and after the aerobic exercise protocol and immediately before and after the anaerobic exercise protocol. Each sample will consist of approximately 8 mL of blood collected from an antecubital vein by qualified healthcare professionals under medical supervision. The samples will be centrifuged at 3,500 revolutions per minute to separate serum and plasma. Serum thyroid-stimulating hormone (TSH), triiodothyronine (T3), thyroxine (T4), and cortisol concentrations will be measured using chemiluminescence-based laboratory methods.
Exercise testing will be conducted at the Hasan Doğan School of Physical Education and Sports Laboratory at Karabuk University. Laboratory analyses will be performed at the Public Health Laboratory of the Karabuk Provincial Health Directorate. Pre-to-post-exercise changes in hormone concentrations will be evaluated within each exercise condition and compared between the aerobic and anaerobic exercise protocols.
1,273 studies on the registry are indexed under Sedentary Behavior; 300 are open to participants now.
This study's planned enrollment of 14 is below the median of 60 across 1,076 interventional studies indexed under Sedentary Behavior.
Browse Sedentary Behavior studies →This is the only study on the registry with Assoc. Prof. Dr. Özgür EKEN as lead sponsor.
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Informed consent
Before you join, the study team explains what taking part involves — its purpose, what happens, the risks and possible benefits — and answers your questions, so you can decide freely. Consent is usually recorded on a form (the informed consent form, ICF) signed by you or, where the law allows, by someone legally authorised to decide for you.
You can keep asking questions during the study, you are told of new information that matters, and you can choose to leave at any time.
Explained by clinicaltrials.gg, not part of the registry record. The study team decides who can take part.
Participants will complete both a progressive 20-meter shuttle run test and a 30-second Wingate anaerobic test in separate testing sessions. Venous blood samples will be collected immediately before and after each exercise protocol to assess acute changes in serum thyroid-stimulating hormone (TSH), triiodothyronine (T3), thyroxine (T4), and cortisol concentrations. Each participant will serve as her own control.
Behavioral: 20-Meter Shuttle Run Test · Behavioral: Wingate Anaerobic Test
Participants will perform a progressive 20-meter shuttle run test by running continuously between two lines positioned 20 meters apart in time with prerecorded audio signals. The initial running speed will progressively increase as the interval between audio signals decreases. The test will end when the participant fails to reach the designated line before the signal on two consecutive occasions.
Participants will perform a 30-second Wingate anaerobic test on a Monark cycle ergometer connected to computerized analysis software. Following an unloaded acceleration phase of approximately 3-4 seconds, a resistance corresponding to 65 g/kg of body mass will be applied. Participants will be instructed to pedal against this resistance at maximal effort for 30 seconds. Peak power, mean power, minimum power, and fatigue index will be recorded.
Change in Serum Cortisol Concentration
Time frame: Immediately before and immediately after each exercise protocol
Plan to share: No — Individual participant data will not be shared because no data-sharing plan was included in the approved study protocol or informed consent process.
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