An interventional study of Walking Protocol and Stair Climbing Protocol in Sedentary Behaviors, sponsored by Malatya Turgut Ozal University. Completed at 1 site in Turkey (Türkiye). Open to male participants aged 18 Years to 30 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2026-09-08.
Sponsored by Malatya Turgut Ozal University · Not applicable, Interventional, and Basic science
This study evaluated the acute physiological responses to three different exercise modalities in sedentary young men. Participants completed walking, stair climbing, and functional circuit exercise sessions in a randomized crossover design. Each exercise session lasted 20 minutes and was performed at a target intensity of 50% heart rate reserve (±5 beats/min), with at least 72 hours between sessions.
The study compared acute respiratory, metabolic, and neuromotor responses to the three exercise modalities. Pulmonary function was assessed using spirometry, blood lactate concentration was measured using capillary blood samples, and countermovement jump performance was assessed as an indicator of neuromuscular performance. Heart rate was continuously monitored during each exercise session.
Exclusion Criteria:
Participants completed the Walking Protocol first, followed by the Stair Climbing Protocol and then the Functional Circuit Protocol, with at least 72 hours between sessions.
Behavioral: Walking Protocol · Behavioral: Stair Climbing Protocol · Behavioral: Functional Circuit Protocol
Participants completed the Walking Protocol first, followed by the Functional Circuit Protocol and then the Stair Climbing Protocol, with at least 72 hours between sessions.
Behavioral: Walking Protocol · Behavioral: Stair Climbing Protocol · Behavioral: Functional Circuit Protocol
Participants completed the Stair Climbing Protocol first, followed by the Walking Protocol and then the Functional Circuit Protocol, with at least 72 hours between sessions.
Behavioral: Walking Protocol · Behavioral: Stair Climbing Protocol · Behavioral: Functional Circuit Protocol
Participants completed the Stair Climbing Protocol first, followed by the Functional Circuit Protocol and then the Walking Protocol, with at least 72 hours between sessions.
Behavioral: Walking Protocol · Behavioral: Stair Climbing Protocol · Behavioral: Functional Circuit Protocol
Participants completed the Functional Circuit Protocol first, followed by the Walking Protocol and then the Stair Climbing Protocol, with at least 72 hours between sessions.
Behavioral: Walking Protocol · Behavioral: Stair Climbing Protocol · Behavioral: Functional Circuit Protocol
Participants completed the Functional Circuit Protocol first, followed by the Stair Climbing Protocol and then the Walking Protocol, with at least 72 hours between sessions.
Behavioral: Walking Protocol · Behavioral: Stair Climbing Protocol · Behavioral: Functional Circuit Protocol
Participants performed a 20-minute walking exercise session at a target intensity of 50% heart rate reserve (HRR), maintained within ±5 beats/min of the individual target heart rate. Heart rate was continuously monitored throughout the exercise session.
Also known as: WP
Participants performed a 20-minute stair climbing exercise session at a target intensity of 50% heart rate reserve (HRR), maintained within ±5 beats/min of the individual target heart rate. Heart rate was continuously monitored throughout the exercise session.
Also known as: SP
Participants performed a 20-minute functional circuit exercise session at a target intensity of 50% heart rate reserve (HRR), maintained within ±5 beats/min of the individual target heart rate. The circuit consisted of chair sit-to-stand, reverse lunges, wall push-ups, high-knee marching, and low-impact mountain climbers performed on an elevated surface. Heart rate was continuously monitored throughout the exercise session.
Also known as: FCP
Change in Forced Expiratory Volume in One Second (FEV1)
Forced expiratory volume in one second (FEV1) was measured by spirometry before and 5 minutes after each exercise condition. The primary outcome was the change in FEV1 (ΔFEV1), calculated as post-exercise FEV1 minus pre-exercise FEV1 and expressed in liters (L). Measurements were obtained following standardized spirometry procedures.
Time frame: Baseline and 5 minutes after each exercise session
Change in Forced Vital Capacity (FVC)
Forced vital capacity (FVC) was measured by spirometry before and 5 minutes after each exercise condition. The outcome was the change in FVC (ΔFVC), calculated as post-exercise FVC minus pre-exercise FVC and expressed in liters (L).
Time frame: Baseline and 5 minutes after each exercise session
Change in FEV1/FVC Ratio
The FEV1/FVC ratio was assessed by spirometry before and 5 minutes after each exercise condition. The outcome was the change in FEV1/FVC ratio (ΔFEV1/FVC), calculated as the post-exercise value minus the pre-exercise value.
Time frame: Baseline and 5 minutes after each exercise session
Change in Peak Expiratory Flow (PEF)
Peak expiratory flow (PEF) was measured by spirometry before and 5 minutes after each exercise condition. The outcome was the change in PEF (ΔPEF), calculated as the post-exercise PEF minus the pre-exercise PEF and expressed in liters per second (L/s).
Time frame: Baseline and 5 minutes after each exercise session
Change in Blood Lactate Concentration
Capillary blood lactate concentration was measured before exercise and 3 minutes after each exercise condition. The outcome was the change in blood lactate concentration (ΔLactate), calculated as the post-exercise value minus the pre-exercise value and expressed in millimoles per liter (mmol/L).
Time frame: Baseline and 3 minutes after each exercise session
Change in Countermovement Jump Height (CMJ)
Countermovement jump (CMJ) height was assessed before and after each exercise condition. The outcome was the change in CMJ height (ΔCMJ), calculated as the post-exercise value minus the pre-exercise value and expressed in centimeters (cm).
Time frame: Baseline and immediately after post-exercise spirometry initiated at 5 minutes after each exercise session
Mean Heart Rate During Exercise
Heart rate was continuously monitored throughout each exercise condition. Mean heart rate was calculated across the exercise period and expressed in beats per minute (bpm).
Time frame: During each 20-minute exercise session
Maximum Heart Rate During Exercise
Heart rate was continuously monitored throughout each exercise condition. Maximum heart rate was defined as the highest heart rate recorded during the exercise period and expressed in beats per minute (bpm).
Time frame: During each 20-minute exercise session
Time Spent in the Target Heart Rate Range
Heart rate was continuously monitored throughout each exercise condition. The outcome was the percentage of exercise time during which heart rate remained within ±5 beats/min of the individualized target heart rate corresponding to 50% heart rate reserve (HRR)
Time frame: During each 20-minute exercise session
Plan to share: No — Individual participant data are not planned to be shared because no data-sharing plan was established as part of the original study protocol.
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Malatya Turgut Ozal University