A Phase 1 interventional study of Transcranial Photobiomodulation in Healthy Volunteers (HV), Transcranial Photobiomodulation and Cortical Excitability, sponsored by Hospital de Clinicas de Porto Alegre. Not yet recruiting at 1 site in Brazil. Open to participants aged 18 Years to 35 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2026-07-28.
Sponsored by Hospital de Clinicas de Porto Alegre · Phase 1, Interventional, and Device feasibility
This study aims to investigate the frequency-dependent effects of near-infrared (NIR) transcranial photobiomodulation (tPBM) on human cortical excitability and fine motor performance. A cohort of 20 healthy adult volunteers (aged 18-35 years) will participate in a randomized, double-blind, sham-controlled, single-center study utilizing a within-subject, five-arm crossover design. Each participant will undergo five distinct experimental conditions separated by a strict washout period: active Continuous Wave (CW) tPBM, active pulsed tPBM at three neural oscillation frequencies (10 Hz, 40 Hz, and 100 Hz), and an inactive Sham comparator. All interventions will be delivered using a clinical-grade near-infrared device.
To isolate the specific effects of pulse frequency from overall energy delivery, the total energy dose (cumulative Joules) and total exposure time will be held perfectly constant across all active arms. The pulsed conditions will keeps the average irradiance and cumulative fluence identical to the continuous wave mode. Cortical excitability will be assessed via single- and paired-pulse Transcranial Magnetic Stimulation (TMS) protocols over the primary motor cortex (M1). Fine motor performance will be quantified using a smartphone-based Finger Tapping Test (FTT). Safety and systemic parameters will be tracked using the Systematic Assessment for Treatment Emergent Events - Systematic Inquiry (SAFTEE-SI) and vital signs. The central hypothesis is that distinct modulation frequencies will differentially influence corticospinal excitability and motor execution, independent of the total energy delivered.
Transcranial photobiomodulation (tPBM) has emerged as a promising non-invasive tool to modulate neural activity through the absorption of near-infrared (NIR) photons by mitochondrial cytochrome c oxidase (CCO). However, whether the biological effects on the human cortex are driven solely by cumulative energy delivery or are significantly modulated by pulse frequency remains a critical question in neurophysiology. This study implements a rigorous within-subject crossover design to systematically isolate the effects of pulse frequency from total dosimetric parameters. Participants and Screening: A sample of 20 healthy volunteers (aged 18-35) will be recruited. Potential candidates will undergo a strict screening protocol to ensure safety and baseline homogeneity. Exclusion criteria include any contraindications to magnetic fields assessed by the Transcranial Magnetic Stimulation Adult Safety Screen (TASS; Rossi et al., 2021), psychiatric conditions according to DSM-5, a history of neurological disorders, the use of psychotropic medications within the last 12 months, or prior participation in an interventional neuromodulation study within the preceding 6 months. Experimental Design \& Intervention: Enrolled participants will complete five experimental sessions in a randomized, counterbalanced order to eliminate carryover or sequence effects, separated by a mandatory washout period to ensure the return of cortical excitability to baseline. In each session, a clinical-grade near-infrared system will be applied over the primary motor cortex (M1) hot spot. The five experimental arms consist of:
Continuous Wave (CW) active tPBM at baseline irradiance. 10 Hz near-infrared active tPBM. 40 Hz near-infrared active tPBM. 100 Hz near-infrared active tPBM. Sham Comparator (inactive control). Physical Calibration and Blinding: To ensure flawless dosimetric matching, total energy dose (Joules) and application duration will be kept constant across all active arms. The active pulsed interventions (10 Hz, 40 Hz, and 100 Hz) will utilize a 50% duty cycle with a peak irradiance exactly twice that of the Continuous Wave (CW) mode, thereby ensuring that the average irradiance (mW/cm2) and cumulative fluence (J/cm2) remain strictly identical to the CW. For the Sham condition, the device will emit a minimal, sub-therapeutic visual output of visible red light. This serves as a visual guide mimic to maintain complete participant blinding without transferring active near-infrared photons to the cortex (0 J active NIR dose). Double-blinding will be enforced for the participant and investigator. Alphanumeric codes will mask the active protocols on the user interface, and participants will wear opaque safety goggles. Outcome Measures: Multiple neurophysiological, behavioral, and safety endpoints will be collected immediately pre-intervention (baseline) and post-intervention. Neurophysiology (TMS): Motor Evoked Potential (MEP) amplitude (primary excitability index); Intracortical Facilitation (ICF); Short-Interval Intracortical Inhibition (SICI); and Cortical Silent Period (CSP). Behavioral Performance (FTT): Evaluated via a dedicated Android application tracking total number of taps, variability of the inter-tap interval (vITI), spatial resultant sum (Σ\|\|Δr\|\|), and the 95% confidence ellipse area (X,Y).
Safety and Hemodynamics: Systemic tolerability will be recorded via the Systematic Assessment for Treatment Emergent Events - Systematic Inquiry (SAFTEE-SI). Hemodynamic variations will be closely monitored through independent analyses of Systolic Blood Pressure (SBP), Diastolic Blood Pressure (DBP), and Heart Rate (HR). Data will be processed using Linear Mixed-Effects Models to account for the repeated-measures structure of the crossover design.
Hospital de Clinicas de Porto Alegre is the lead sponsor of 450 studies on the registry; 61 are open to participants now.
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Exclusion Criteria:
Active near-infrared (NIR) transcranial photobiomodulation delivered in Continuous Wave (CW) mode using the NIR device, providing a constant baseline irradiance to achieve the target total energy dose (J).
Combination Product: Transcranial Photobiomodulation
Active NIR light delivered in pulsed mode at a frequency of 10 Hz. It matched across frequencies to ensure that the average irradiance (mW/cm2) and total energy dose (J) remain identical to the CW baseline within the same exposure time.
Combination Product: Transcranial Photobiomodulation
Active NIR light delivered in pulsed mode at a frequency of 40 Hz. It matched across frequencies to ensure that the average irradiance (mW/cm2) and total energy dose (J) remain identical to the CW baseline within the same exposure time.
Combination Product: Transcranial Photobiomodulation
Active NIR light delivered in pulsed mode at a frequency of 100 Hz. It matched across frequencies to ensure that the average irradiance (mW/cm2) and total energy dose (J) remain identical to the CW baseline within the same exposure time.
Combination Product: Transcranial Photobiomodulation
Inactive transcranial photobiomodulation (tPBM) delivered near-infrared device. To ensure successful participant blinding, the device will be programmed to deliver a minimal, non-therapeutic power output of visible red light (660 nm), which serves as a visual guide mimic but delivers negligible energy to the cortical tissue (0 J of active near-infrared light). Total exposure time, acoustic signals, and equipment interface will be identical to the active arms.
Combination Product: Transcranial Photobiomodulation
Transcranial photobiomodulation (tPBM) is a non-invasive, non-thermal neuromodulatory modality that utilizes low-power coherent (laser) or non-coherent (light-emitting diodes, LEDs) light sources within the red (lambda = 600-700 nm) and near-infrared (NIR; lambda = 700-1100 nm) spectral windows to modulate cortical function. Structurally tailored to penetrate superficial anatomical barriers-including the scalp, skull, and meninges-tPBM delivers photons directly to the cerebral cortex.
Motor Evoked Potential (MEP) Amplitude - TMS
Single-pulse TMS will be applied over the primary motor cortex (M1) hotspot to elicit Motor Evoked Potentials (MEPs) recorded via electromyography (EMG) from the target muscle (e.g., first dorsal interosseous, FDI). Peak-to-peak MEP amplitude (mV) will be measured at a stimulation intensity adjusted to evoke a baseline response of approximately 1 mV. This outcome reflects overall baseline corticospinal excitability and its modulation following the tPBM protocol.
Time frame: Immediately before the intervention (pre-intervention) and 20 minutes after the intervention (post-intervention)
Intracortical Facilitation (ICF) - TMS
Evaluated using a paired-pulse TMS protocol consisting of a subthreshold conditioning stimulus (80% of resting motor threshold, RMT) followed by a suprathreshold test stimulus (120% RMT) at a long interstimulus interval (ISI) of 10 ms. The outcome is expressed as the ratio of the conditioned MEP amplitude to the unconditioned test MEP amplitude. ICF is primarily mediated by cortical glutamatergic circuits and NMDA receptor activity.
Time frame: Immediately before the intervention (pre-intervention) and 20 minutes after the intervention (post-intervention)
Short-Interval Intracortical Inhibition (SICI) - TMS
Assessed via a paired-pulse TMS paradigm using a subthreshold conditioning stimulus (80% RMT) followed by a suprathreshold test stimulus (120% RMT) at a short interstimulus interval (ISI) of 3 ms. The resulting SICI value is quantified as the percentage of inhibition of the conditioned MEP relative to the unconditioned test MEP. This parameter indexes local intracortical inhibitory interneuron activity mediated by GABA\_A receptors.
Time frame: Immediately before the intervention (pre-intervention) and 20 minutes after the intervention (post-intervention)
Cortical Silent Period (CSP) - TMS
Induced by applying a single suprathreshold TMS pulse (120% RMT) over the M1 hotspot while the participant maintains a stable, isometric voluntary contraction of the target muscle (e.g., 20% of maximum voluntary contraction). The CSP duration (ms) is measured from the onset of the MEP to the return of rectified background EMG activity. CSP duration provides a precise marker of interhemispheric and intracortical inhibition mediated by GABA\_B receptors.
Time frame: Immediately before the intervention (pre-intervention) and 20 minutes after the intervention (post-intervention)
Total Number of Taps - FTT
The cumulative count of valid screen contacts executed by the participant's index finger within a fixed, standardized testing interval (e.g., 30 seconds). This metric serves as a behavioral index of maximal motor execution speed and tapping frequency.
Time frame: Immediately before the intervention (pre-intervention) and immediately after the intervention (post-intervention)
Variability of the Inter-Tap Interval (vITI) - FTT
Calculated as the standard deviation (or coefficient of variation) of the temporal intervals between consecutive screen contacts (in milliseconds). This outcome quantifies the temporal rhythmic precision and stability of the central motor program.
Time frame: Immediately before the intervention (pre-intervention) and immediately after the intervention (post-intervention)
Spatial Resultant Sum (Σ||Δr||) - FTT
The cumulative Euclidean distance calculated across all sequential tap coordinates on the 2D Android screen interface. This parameter reflects spatial dispersion and motor drift, tracking the continuous precision of the targeted finger-pointing trajectory.
Time frame: Immediately before the intervention (pre-intervention) and immediately after the intervention (post-intervention)
95% Confidence Ellipse Area (X,Y) - FTT
Computed as the total geometric surface area (in squared millimeters, mm2) of the bivariate error ellipse that encompasses 95% of the coordinates of all performed taps on the horizontal (X) and vertical (Y) axes. This spatial metric quantifies overall motor accuracy and targeting consistency.
Time frame: Immediately before the intervention (pre-intervention) and immediately after the intervention (post-intervention)
Adverse Events and Tolerability (SAFTEE-SI)
The safety and tolerability profile of the combined neuromodulation protocol will be systematically evaluated using the Systematic Assessment for Treatment Emergent Events - Systematic Inquiry (SAFTEE-SI). This structured instrument will track the incidence, severity, and potential causal relationship of any somatic, neurological, or behavioral symptoms (e.g., headache, scalp discomfort, fatigue, dizziness, or localized thermal sensations) emerging during or after stimulation.
Time frame: Baseline, before all interventions and one week after the intervention
Systolic Blood Pressure (SBP)
Systemic systolic blood pressure will be quantified in millimeters of mercury (mmHg) using a calibrated, digital oscillometric upper-arm sphygmomanometer. Measurements will be recorded under standardized resting conditions at pre-intervention (baseline) and immediately post-intervention across all five crossover arms to monitor potential acute alterations in peak arterial pressure induced by transcranial photobiomodulation.
Time frame: Immediately before the intervention (pre-intervention) and immediately after the intervention (post-intervention)
Diastolic Blood Pressure (DBP)
Systemic diastolic blood pressure will be quantified in millimeters of mercury (mmHg) using the same automated oscillometric system. Measurements will be recorded concurrently with the systolic parameters at baseline and immediately post-intervention across all five crossover sessions to evaluate potential shifts in minimum arterial pressure during cardiac relaxation.
Time frame: Immediately before the intervention (pre-intervention) and immediately after the intervention (post-intervention)
Heart Rate (HR)
Heart rate will be quantified in beats per minute (bpm) concurrently with the blood pressure evaluation using the same automated digital system. This parameter serves as an acute physiological indicator of autonomic nervous system balance (sympathovagal tone) before and after the application of continuous or sinusoidal pulsed photobiomodulation protocols.
Time frame: Immediately before the intervention (pre-intervention) and immediately after the intervention (post-intervention)
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Hospital de Clinicas de Porto Alegre