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RecruitingNCT07508657LIFTUpdated Sep 9, 2026

Progesterone Supplementation After Letrozole-stimulated Insemination

A Phase 4 interventional study of Progesterone in Infertility, sponsored by Kirstine Kirkegaard. Recruiting at 4 sites in Denmark. Open to female participants aged 18 Years to 37 Years. Per ClinicalTrials.gov, last updated 2026-09-09.

Sponsored by Kirstine Kirkegaard · Phase 4, Interventional, and Treatment

Phase
Phase 4
Study type
Interventional
Enrollment
690
Allocation
Randomized
Ages
18 Years to 37 Years
Sex
Female
01

Study summary

This trial aims to investigate whether luteal phase support improves the chance of pregnancy in women undergoing intrauterine insemination (IUI) following ovarian stimulation with letrozole.

In this randomized clinical trial, 690 women undergoing letrozole-stimulated IUI at four public fertility clinics in Denmark will be randomly allocated to one of two groups:

  • Vaginal progesterone from the day after insemination (Cyclogest 400 mg twice daily)
  • No luteal phase support, reflecting current clinical practice

All participants will undergo a standard letrozole-stimulated IUI treatment, including ultrasound monitoring, ovulation triggering, and insemination. Blood samples will be collected on the day of insemination and 7-9 days after insemination to measure hormone levels.

The results of this trial will provide further insight into the role of progesterone support in letrozole-stimulated IUI cycles.

02

Conditions studied

  • Infertility

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Keywords

  • Luteal phase support
  • Intrauterine insemination
  • Letrozole
  • Vaginal progesterone
  • Randomized clinical trial
  • Ovulation induction
03

Who can participate

Ages eligible
18 Years to 37 Years
Sexes eligible
Female
Accepts healthy volunteers
No

Inclusion criteria

  • Age 18-37 years
  • Scheduled for IUI with homologous or donor sperm
  • Undergoing mild ovarian stimulation with letrozole
  • Ability to provide written informed consent

Exclusion criteria

Exclusion Criteria:

  • Age > 37 years
  • Anovulation due to hypogonadotropic hypogonadism
  • Known hypersensitivity to progesterone or hard fat listed as an excipient in the Summary of Product Characteristics (SmPC) for Cyclogest.
  • Known or suspected progesterone-sensitive malignant tumours
  • Porphyria
  • Known missed abortion or ectopic pregnancy
  • Active arterial or venous thromboembolism, severe thrombophlebitis, or a history of these conditions
  • Severe hepatic dysfunction or liver disease
  • Inability to speak or understand Danish sufficiently to comprehend oral and written study information
04

Study design

Phase
Phase 4
Primary purpose
Treatment
Allocation
Randomized
Intervention model
Parallel assignment
Masking
Single (Outcomes assessor)
Enrollment
690 participants (estimated)

Study arms

  • Experimental
    Intervention

    Vaginal progesterone luteal phase support. Progesterone is administered as Cyclogest 400 mg twice daily form the day after insemination. Treatment is continued until gestational age 10 weeks in the event of clinical pregnancy, or until pregnancy is ruled out in women who do not achieve a clinical pregnancy.

    Drug: Progesterone

  • No intervention
    Comparison

    No luteal phase support will be administered after insemination, reflecting current standard treatment.

Interventions

  • DrugProgesterone

    Vaginal progesterone (Cyclogest) 400 mg administered twice daily starting the day after insemination. Treatment is continued until gestational age 10 weeks in the event of clinical pregnancy or until clinical pregnancy is ruled out.

    Also known as: Cyclogest

05

What researchers measure

Primary outcomes

  1. Clinical Pregnancy Rate

    Defined as an ultrasonographically visible foetal heartbeat at 7-9 weeks of gestation

    Time frame: Gestational age 7-9 weeks

Secondary outcomes

  1. Live Birth Rate

    Defined as the birth of one or more living infants after a pregnancy of GA ≥ 22 weeks

    Time frame: At the time of delivery

  2. Biochemical Pregnancy Rate

    Defined as a positive serum or urine β-hCG test 14-17 days after intrauterine insemination

    Time frame: 14-17 days after intrauterine insemination

  3. Early Pregnancy Loss

    Defined as intrauterine pregnancy loss before 10 weeks of gestational age

    Time frame: Up to 10 weeks of gestation

  4. Fetal miscarriage

    Defined as pregnancy loss ≥ 10 weeks size with a fetus (≥ 33 mm) on ultrasound

    Time frame: From 10 weeks of gestation to delivery

  5. Obstetric outcomes

    Obstetric complications including hypertensive disorders of pregnancy, gestational diabetes, postpartum hemorrhage and cesarean section

    Time frame: At time of delivery

  6. Perinatal outcomes

    Preterm birth, birth weight, congenital malformations, and perinatal mortality (defined as foetal or neonatal death from GA 22+0 to 7 days after birth).

    Time frame: At time of delivery

  7. Hormone levels

    Serum hormone levels measured on the day of insemination, in the mid-luteal phase and on the day of ovulation trigger (optional), including progesterone, estradiol, LH and FSH.

    Time frame: Time of insemination and mid-luteal phase (approximately 7-9 days after insemination).

06

Study locations

1 of 4 sites recruiting
  • Copenhagen University Hospital - Rigshospitalet. Department of Gynecology, Fertility and Obstetrics
    Copenhagen, 2100, Denmark
    • Kristine Løssl, Clinical Associate Professor · Contact · fertilitet.rigshospitalet@regionh.dk · +4535451077
    • Kristine Løssl, Clinical Associate Professor · Principal investigator
    Not yet recruiting
  • The Fertility Clinic, Herlev Hospital
    Herlev, 2730, Denmark
    Not yet recruiting
  • University Clinic for Fertility, Horsens Regional Hospital, Central Denmark Region
    Horsens, 8700, Denmark
    • Kirstine Kirkegaard, Clinical Associate Professor · Contact · fertilitet@horsens.rm.dk · +4578426562
    • Kirstine Kirkegaard, Clinical Associate Professor · Principal investigator
    Recruiting
  • The Fertility Clinic, Hvidovre Hospital
    Hvidovre, 2650, Denmark
    Not yet recruiting
07

References and documents

Individual participant data

Plan to share: No — Individual participant data will not be made avaliable.

No publications or documents are linked to this record.

08

Registry details

Key details

Study ID
NCT07508657
Lead sponsor
Kirstine Kirkegaard
Responsible party
Kirstine Kirkegaard (Clinical Associate Professor, MD, PhD, University of Aarhus) — Sponsor-investigator
First posted
Apr 2, 2026
Start date
Aug 28, 2026
Primary completion
Jun 2029 (estimated)
Completion
Jan 2030 (estimated)
Last update
Sep 9, 2026

Study contacts

Kirstine Kirkegaard, Clinical Associate Professor
Contact
fertilitet@horsens.rm.dk
+4578426562
Kirstine Kirkegaard, Clinical Associate Professor
principal investigator · University Clinic for Fertility, Horsens Regional Hospital, Central Denmark Region

Oversight

Data monitoring committee
No
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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