An interventional study of Beetroot-based antioxidant beverage in Repeated Sprint Performance in Trained Athletes, Exercise Performance and Nitrate Supplementation, sponsored by Universidad Autonoma de Chihuahua. Not yet recruiting. Open to male participants aged 18 Years to 35 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2026-03-16.
Sponsored by Universidad Autonoma de Chihuahua · Not applicable, Interventional, and Other
This randomized, double-blind, placebo-controlled crossover trial aims to evaluate the acute effects of a nitrate-rich beetroot-based beverage on repeated sprint performance in trained athletes.
Ten athletes will complete two experimental conditions in randomized order: beetroot beverage (BET) and placebo (PLA), separated by a 7-day washout period. The beetroot beverage consists of 200 mL of a mixture containing beetroot, cocoa, and green tea, providing approximately 6.4 mmol of nitrate.
In each condition, participants will perform a Running Anaerobic Sprint Test (RAST), consisting of six maximal 35-meter sprints with 10 seconds of passive recovery between sprints. The primary outcome is mean power output across the six sprints.
Secondary outcomes include fatigue index, sprint-by-sprint power output, heart rate, rating of perceived exertion (Borg 6-20 scale), maximal oxygen uptake (VO₂max), and sleep quality assessed using the Insomnia Severity Index (ISI).
This randomized, double-blind, placebo-controlled crossover trial is designed to investigate the acute effects of a nitrate-rich beetroot-based beverage on repeated sprint performance in trained athletes.
Ten athletes will participate in two experimental sessions in randomized order: beetroot beverage (BET) and placebo (PLA). Randomization will determine the sequence of beverage administration. Participants and investigators will be blinded to treatment allocation.
After beverage ingestion, participants will perform a standardized warm-up followed by the Running Anaerobic Sprint Test (RAST), consisting of six maximal 35-meter sprints with 10 seconds of passive recovery between sprints. Sprint time will be recorded using electronic timing, and power output will be calculated using body mass and sprint time.
The primary outcome measure is mean power output across all six sprints. Secondary outcomes include:
Fatigue index (percentage decrement across sprints)
Individual sprint power (S1-S6)
Heart rate response
Rating of perceived exertion (Borg 6-20 scale)
VO₂max (assessed at baseline)
Sleep quality measured using the Insomnia Severity Index (ISI)
A linear mixed-effects model will be used to analyze repeated sprint performance, including subject as a random intercept and treatment, sprint, and their interaction as fixed effects. Non-parametric tests will be applied when assumptions of normality are not met. Statistical significance will be set at p ≤ 0.05.
Universidad Autonoma de Chihuahua is the lead sponsor of 4 studies on the registry; 3 are open to participants now.
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Inclusion Criteria:
Age between 18-35 years
Regular training ≥3 times/week
No cardiovascular or metabolic disease
Exclusion Criteria:Healthy trained athletes
Age between 18-35 years
Regular training ≥3 times/week
No cardiovascular or metabolic disease
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Participants consumed 200 mL of a beetroot-based antioxidant beverage containing beetroot, cocoa, and green tea extract, providing approximately 6.4 mmol of dietary nitrate. The beverage was ingested 2-3 hours before performing the exercise test.
Dietary Supplement: Beetroot-based antioxidant beverage
Participants consumed 200 mL of a placebo beverage matched for color, taste, and texture but without nitrate or active antioxidant compounds. The beverage was ingested 2-3 hours before performing the exercise test.
Dietary Supplement: Beetroot-based antioxidant beverage
Participants consumed 200 mL of a beetroot-based antioxidant beverage containing beetroot, cocoa, and green tea extract, providing approximately 6.4 mmol of dietary nitrate. The beverage was administered 2-3 hours before performing a Running Anaerobic Sprint Test (RAST). The formulation was designed to deliver nitrate and polyphenols with potential ergogenic and antioxidant effects. A 7-day washout period separated crossover conditions.
Mean power output (W) during six maximal 35-m sprints
Mean power output will be calculated from six maximal 35-m sprint efforts separated by standardized recovery intervals. Sprint time will be recorded and converted to power output (W). The mean value across all six sprints will be used as the outcome measure.
Time frame: Baseline and immediately post-intervention.
No study locations are listed for this record.
Plan to share: No
This study is not yet recruiting, as verified in Mar 2026. You cannot join it, but the record below documents what was studied.
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Universidad Autonoma de Chihuahua