An interventional study of Exercise intervention in Sedentary Behavior, Healthy Participants and Adult, sponsored by Shenzhen Institutes of Advanced Technology ,Chinese Academy of Sciences. Recruiting at 1 site in China. Open to participants aged 18 Years to 40 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2026-01-21.
Sponsored by Shenzhen Institutes of Advanced Technology ,Chinese Academy of Sciences · Not applicable, Interventional, and Basic science
The goal of this clinical trial is to study the effectiveness of aerobic exercise in treating obesity in adults and to assess the efficacy of exercise. The primary research questions it seeks to address are as follows:
Participants will engage in the following activities:
Based on previous research findings, scientists found that exercise results in energy compensation. In a non-interventional setting, energy intake and expenditure undergo a dynamic equilibrium process. Nonetheless, the energy deficit induced by exercise is offset by other physiological or psychological behaviors, leading to a diminished energy discrepancy caused by exercise, thereby mitigating the weight loss effects typically associated with exercise(Pontzer et al 2016: Current Biology).Controlled dietary experiments have indicated that long-term exercise results in approximately 22% energy compensation (Bouchard et al 1994: Obesity Research); however, the underlying source and mechanism of this compensation remains ambiguous. Analyses of Total Energy Expenditure (TEE) in 1754 individuals have demonstrated that the energy compensation from exercise primarily emanates from a reduction in Basal Energy Expenditure (BEE), with only an average of 72% of the energy expended during exercise being utilized for additional energy expenditure (Careau et al 2021: Current Biology). Nevertheless, this deduction is restricted by the measurements of energy intake, Adaptive thermogenesis, Thermic Effect of Food (TEF), and Activity-related Energy Expenditure (AEE), emphasizing the necessity of exercise intervention studies to comprehensively grasp the pathways and mechanisms of energy compensation.
To tackle the challenges posed by overweight and obesity, enhance residents' quality of life, and delve deeper into the intricacies of energy compensation, this research project focuses on examining the metabolic reactions of the body to exercise interventions. Through the analysis of pre- and post-exercise energy expenditure and intake, body composition, metabolism, microbiota, and metabolomics alterations in humans, the primary goal is to thoroughly elucidate the principal factors and mechanisms behind energy compensation. This clinical trial consists of four phases: pre-exercise intervention baseline measurement, exercise intervention, Immediately after 12 weeks' exercise intervention measurement, and follow-up measurement 8 weeks post-completion of the exercise intervention.
Pre-exercise intervention baseline measurement:
Anthropometric measurements: body weight, height, and body composition
Body composition:
Metabolic measurements: blood pressure, glucose, heart rate, energy expenditure, and cardiovascular function
Energy expenditure：
Behaviour monitor: physical activity and food intake.
Exercise intervention:
Immediately measurement after 12 weeks'exercise intervention (same as pre-exercise intervention baseline measurement):
Follow-up measurement 8 weeks post-completion of the exercise intervention (same as pre-exercise intervention baseline measurement):
332 studies on the registry are indexed under Sedentary Behavior; 155 are open to participants now.
This study's planned enrollment of 80 is above the median of 70 across 287 interventional studies indexed under Sedentary Behavior.
Browse Sedentary Behavior studies →Shenzhen Institutes of Advanced Technology ,Chinese Academy of Sciences is the lead sponsor of 21 studies on the registry; 15 are open to participants now.
Counted across the registry records on this site, refreshed daily.
Exclusion Criteria:
Participants will run on treadmill under the supervision of the researcher * Intensity:60% of VO2max * Duration: for 12 weeks * Frequency: 5 times a week.
Behavioral: Exercise intervention
Exercise prescription for participant， * 150Kcal for Weeks 1-4 (gradually increase to 150Kcal in the first two weeks as familiar stage), * 250Kcal for Weeks 5-8, * 350Kcal for Weeks 9-12.
Also known as: physical activity
Change in TEE.
The change in TEE will be evaluated using the DLW method before and after exercise intervention.
Time frame: Three times, it takes 14 days for each time: before exercise intervention (week 0), immediately after exercise intervention (week 12), and 8 weeks post-completion of exercise intervention (week 20).
Change in TEF and REE.
The change in BEE and TEF will be measured through indirect calorimetry in a respiratory chamber.
Time frame: Three times, it takes 1 days for each time: before exercise intervention (week 0), immediately after exercise intervention (week 12), and 8 weeks post-completion of exercise intervention (week 20).
Change in exercise energy expenditure.
The change in exercise energy expenditure will be assessed using indirect calorimetry with a respiratory hood system (COSMED).
Time frame: Three times, it takes 14 days for each time: before exercise intervention (week 0), immediately after exercise intervention (week 12), and 8 weeks post-completion of exercise intervention (week 20).
Change in food intake.
Food intake will be assessed using a 2-day food record complemented by photographing and weighing the food before and after consumption.
Time frame: Three times, food intake assessment takes 2 days: before exercise intervention (week 0), immediately after exercise intervention (week 12), and 8 weeks post-completion of exercise intervention (week 20).
Change in physical activity.
The physical activity levels of the participants will be monitored through the use of a GT3X accelerometer.
Time frame: Three times, it takes takes 14 days: before exercise intervention (week 0), immediately after exercise intervention (week 12), and 8 weeks post-completion of exercise intervention (week 20).
Change in fasting glucose and postprandial glucose.
The continuous glucose monitoring system (Medtronic) will record the glucose concentration in mmol/L for 7 days.
Time frame: Three times, 7 days per each time: before exercise intervention (week 0), immediately after exercise intervention (week 12), and 8 weeks post-completion of exercise intervention (week 20).
Change in circulating hormones and metabolites.
During the experiment, the nurse will collect the fasting blood sample in each month throughout exercise intervention, right after the first and last exercise session, and the 8 weeks upon completion of exercise intervention. Metabolomics will analyze the impact of an exercise intervention on metabolites.
Time frame: 8 times, 1 minutes for each collection, 7 days for analysis. Every months (week0, 4, 8,12, and 30), and after exercise energy expenditure test(week 0,12, and 20))
Change in intestinal microbiota.
Feces will be collected for gut microbiome analysis of intestinal microbiota.
Time frame: Three times, it takes 5 minutes for collection, 7 days for analysis. before exercise intervention (week 0), immediately after exercise intervention (week 12), and 8 weeks post-completion of exercise intervention (week 20).
Change in fat mass.
Fat mass will be measured by Magnetic Resonance Imaging (Shanghai united imaging, uMR 790 ).
Time frame: Three times, it takes 10 minutes, before exercise intervention (week 0), immediately after exercise intervention (week 12), and 8 weeks post-completion of exercise intervention (week 20).
Change in fat free mass.
Fat free mass will be measured by Bioimpedance Analysis (Tanita, MC-980).
Time frame: Three times, it takes 2 minutes for each time, before exercise intervention (week 0), immediately after exercise intervention (week 12), and 8 weeks post-completion of exercise intervention (week 20).
Change in bone mass.
Bone mass will be measured by DXA( Horizon Wi, HOLOGIC)
Time frame: Three times, it takes 7 minutes for each time, before exercise intervention (week 0), immediately after exercise intervention (week 12), and 8 weeks post-completion of exercise intervention (week 20).
Change in metabolic energy expenditure before and after an intense exercise session.
Participants will enter the chamber at night to equilibrate the chamber air and become familiar with the setup. The initial light exposure day in the respiratory chamber, labeled as day 0, will not involve any exercise. The subsequent 24 hours will mark the beginning of the exercise intervention, designated as day 1. The alterations of BEE and TEF will be assessed using indirect calorimetry within the respiratory chamber between day 0 and day 1.
Time frame: 1 day, before and after the first intense exercise session
Change in metabolic energy expenditure upon completion of exercise intervention.
Participants will enter the chamber at the second last exercise intervention night to equilibrate the chamber air and become familiar with the setup. The initial light exposure day in the respiratory chamber, labeled as day 84, will be the last day of exercise intervention. The subsequent 24 hours will mark the first day of the completion of exercise intervention, designated as day 85. The alterations of BEE and TEF will be assessed using indirect calorimetry within the respiratory chamber between day 84 and day 85.
Time frame: 1 day, before and after the last exercise session
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Shenzhen Institutes of Advanced Technology ,Chinese Academy of Sciences