CClinicalTrials.gg
RecruitingNCT06902480Updated Sep 29, 2026

Dairy Choline Bioavailability

An interventional study of WPPC and Whole egg powder in Post-menopausal Women and Post-menopausal Status, sponsored by University of Wisconsin, Madison. Recruiting at 1 site in United States. Open to female participants aged 51 Years to 70 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2026-09-29.

Sponsored by University of Wisconsin, Madison · Not applicable, Interventional, and Other

Phase
Not applicable
Study type
Interventional
Enrollment
24
Allocation
Randomized
Ages
51 Years to 70 Years
Sex
Female
01

Study summary

The goal of this clinical trial is to find out if dairy is a good source of choline compared to eggs. The main questions it aims to answer are:

  • How the body uses choline; and,
  • What effect WPPC has on choline metabolism.

Researchers will compare Whey Protein Phospholipid Concentrate (WPPC) to whole egg powder to see if WPPC is as effective as eggs.

Participants will drink a chocolate-flavored smoothie mixed with either WPPC or whole egg powder.

Read the detailed description

This study is intended to evaluate the bioavailability and metabolism of choline in WPPC compared to the most commonly -used food for bioavailable choline, whole egg. The intent of the evaluation is to improve the knowledge of how WPPC contributes to the nutritional needs and health of postmenopausal women.

02

Conditions studied

  • Post-menopausal Women
  • Post-menopausal Status
03

Who can participate

Ages eligible
51 Years to 70 Years
Sexes eligible
Female
Accepts healthy volunteers
Yes

Inclusion criteria

  • Ages between 51 and 70
  • Is currently post-menopausal (Post-menopausal is a term used to describe the time in a woman's life after she has gone through menopause. This usually happens between the ages of 45 and 55. Menopause is when a woman's menstrual periods stop permanently (at least 12 months))
  • Not allergic to the intervention foods and allergens that are related to the production of intervention foods (e.g. dairy products, egg, fish, peanut, shellfish, soy, tree nuts, wheat, crustaceans, gluten, mollusks, mustard, sesame seeds.)
  • Had stable body weight (± 5%) for the past 6 months
  • Free from cancer
  • Did not have weight loss surgery, colon surgery
  • Do not have chronic kidney diseases
  • Do not have malabsorption towards fat and dairy products
  • Willing to complete health surveys
  • Willing to complete two 4-day food diaries
  • Willing to consume the intervention smoothies
  • Willing to provide blood, urine, fecal samples at baseline and after 10 days of intervention

Exclusion criteria

Exclusion Criteria:

  • On weight loss/weight gain diets
  • Diagnosed with fat malabsorption
  • Undergoing weight loss surgery
  • Diagnosed with chronic kidney diseases
  • Allergic to the intervention foods and allergens that are related to the production of intervention foods (e.g. dairy products, egg, chocolate, fish, peanut, shellfish, soy, tree nuts, wheat, crustaceans, gluten, mollusks, mustard, sesame seeds, banana, strawberry, raspberry, blackberry, blueberry.)
  • Regularly using antibiotics or probiotics or prebiotics
  • Regularly using weight-loss drugs (GLP-1, Orlistat, Cetilistat, etc.)
  • Regularly using laxatives
  • Unable to avoid strenuous exercise during the study (>4 h vigorous exercise per day)
04

Study design

Phase
Not applicable
Primary purpose
Other
Allocation
Randomized
Intervention model
Parallel assignment
Masking
Single (Participant)
Enrollment
24 participants (estimated)

Study arms

  • Experimental
    Whey Protein Phospholipid Concentrate (WPPC)

    Participants will drink a chocolate-flavored smoothie with WPPC added

    Dietary Supplement: WPPC

  • Experimental
    Pasteurized whole egg powder

    Participants will drink a chocolate-flavored smoothie with egg powder added

    Dietary Supplement: Whole egg powder

Interventions

  • Dietary supplementWPPC

    A chocolate-flavored smoothie with WPPC added

  • Dietary supplementWhole egg powder

    A chocolate-flavored smoothie with whole egg powder added

05

What researchers measure

Primary outcomes

  1. Change in circulating choline

    Circulating choline will be measured by UHPLC-HRMS/MS

    Time frame: Baseline to 10 days

Secondary outcomes

  1. Change in co-factors in circulating choline

    Co-factors in the circulation will be measured by ultrahigh performance liquid chromatography coupled with high resolution tandem mass spectrometry (UHPLC-HRMS/MS).

    Time frame: Baseline to 10 days

  2. Change in high-sensitivity C-reactive protein (hs-CRP)

    Safety biomarker Hs-CRP will be measured using immunoturbidimetry.

    Time frame: Baseline to 10 days

  3. Change in circulating choline metabolites

    Metabolites will be measured by UHPLC-HRMS/MS.

    Time frame: Baseline to 10 days

  4. Change in 8-hydroxydeoxyguanosine

    The safety biomarker 8-hydroxydeoxyguanosine (8-OHDG) will be measured using enzyme-linked immunosorbent assay (ELISA).

    Time frame: Baseline to 10 days

  5. Change in aspartate transaminase

    Aspartate transaminase (AST) will be measured using kinetic methods.

    Time frame: Baseline to 10 days

  6. Change in alanine transaminase (ALT)

    Alanine transaminase will be measured using kinetic methods.

    Time frame: Baseline to 10 days

  7. Change in creatinine

    Creatinine will be measured using kinetic methods

    Time frame: Baseline to 10 days

06

Study locations

1 of 1 sites recruiting
  • University of Wisconsin - Madison
    Madison, Wisconsin 53705, United States
    • Kevin Shih · Contact · sshih22@wisc.edu
    • Bradley Bolling, PhD · Principal investigator
    Recruiting
07

References and documents

Individual participant data

Plan to share: Yes — The research team will share the individual participant data collected during the trial after deidentification. Deidentified data will be stored and shared with DryAd and in accordance with all applicable regulations.

No publications or documents are linked to this record.

08

Registry details

Key details

Study ID
NCT06902480
Lead sponsor
University of Wisconsin, Madison
Responsible party
Sponsor
First posted
Mar 30, 2025
Start date
Sep 18, 2026
Primary completion
Dec 2027 (estimated)
Completion
Dec 2027 (estimated)
Last update
Sep 29, 2026

Study contacts

Kevin Shih
Contact
sshih22@wisc.edu
919-338-9453
Bradley Bolling, PhD
principal investigator · University of Wisconsin, Madison

Oversight

Data monitoring committee
Yes
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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