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RecruitingNCT06771479Updated Oct 3, 2025

A Study Evaluating the Safety and Immunogenicity of MVA Strain Monkeypox Attenuated Live Vaccine

A Phase 1 interventional study of MVA strain monkeypox attenuated live vaccine (low dose) and MVA strain monkeypox attenuated live vaccine (high dose) in Monkeypox, sponsored by Shanghai Institute Of Biological Products. Recruiting at 1 site in China. Open to participants aged 18 Years and older, including healthy volunteers. Per ClinicalTrials.gov, last updated 2025-10-03.

Sponsored by Shanghai Institute Of Biological Products · Phase 1, Interventional, and Prevention

Phase
Phase 1
Study type
Interventional
Enrollment
120
Allocation
Randomized
Ages
18 Years and older
Sex
All
01

Study summary

This is a randomized, double blinded, controlled phase I clinical study. To evaluate the safety and immunogenicity of receiving two doses of MVA strain monkeypox attenuated live vaccine in individuals aged 18 years and above.

Read the detailed description

This is a randomized, double blinded, controlled phase I clinical study. The study plans to recruit 120 participants aged 18 years and above. The experimental vaccine MVA strain monkeypox attenuated live vaccine has two different doses (low dose and high dose). The ratio of low-dose experimental group, high-dose experimental group, and placebo group is 1:1:1. Participants will be divided into three groups: healthy individuals, men who have sex with men, and HIV infected individuals, with a ratio of 2:1:1. Different groups will be divided into individuals with a history of vaccination against smallpox and those without a history of immunization, based on whether each population has a history of vaccination against smallpox. Each participant will receive one dose of the experimental vaccine or placebo on day 0 and day 28, respectively.

02

Conditions studied

  • Monkeypox

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Keywords

  • Monkeypox vaccine
  • safety
  • vaccine
  • immunogenicity
  • Monkeypox
03

Who can participate

Ages eligible
18 Years and older
Sexes eligible
All
Accepts healthy volunteers
Yes

Inclusion criteria

  • On the day of enrollment, individuals aged 18 years or older with a history of smallpox vaccination must have been born before 1980;
  • Can provide legal proof of identity;
  • Be able to understand the experimental procedure and sign a written informed consent form, expressing agreement to participate in the experiment;
  • Be able to participate in all planned follow-up visits and comply with all trial procedures (such as completing diary/contact cards and being able to return for visits);
  • On the day of enrollment, the body temperature was less than 37.3 ℃ (axillary temperature);
  • Men and women of childbearing age who have no plans to have children within 6 months and agree to take effective contraceptive measures within 6 months after receiving the experimental vaccine.

Exclusion criteria

Exclusion Criteria:

  • Individuals with a history of smallpox, monkeypox, or close contact with monkeypox in the past;
  • Individuals who are allergic to any ingredients of eggs, vaccines, or substances used in production processes, or have a history of other severe allergies;
  • Use immunoglobulin and/or any blood products within 3 months prior to administering the trial vaccine, or plan to use them during the trial period;
  • Currently using salicylate drugs or planning long-term use during the trial period;
  • Have used any experimental or unregistered products within one month prior to administering the trial vaccine, or plan to use them during the trial period;
  • Administer inactivated vaccine within 14 days before administering the experimental vaccine or attenuated live vaccine within 30 days before administering the experimental vaccine;
  • Chronic disease patients are in the acute or progressive phase of chronic disease;
  • Long term use of immunosuppressants or other immunomodulatory drugs within 6 months prior to vaccination with the experimental vaccine;
  • Having undergone chemotherapy or radiation therapy or organ and bone marrow transplantation related treatments for cancer or other diseases;
  • Diseases or medical measures that lead to immune dysfunction, such as congenital immunodeficiency, organ and bone marrow transplantation, leukemia, lymphoma, Hodgkin's disease, multiple myeloma or malignant tumors, etc;
  • Moderate or severe acute illness/infection, or febrile illness on the day of vaccination;
  • Individuals with a history of thrombocytopenia or other coagulation disorders may be contraindicated for subcutaneous injection;
  • Suffering from serious cardiovascular disease, serious liver and kidney disease, and diabetes that cannot be controlled by drugs;
  • Previous history of mental or neurological disorders or family history;
  • Currently suffering from various infectious, suppurative, and allergic skin diseases;
  • Women of childbearing age: pregnant or lactating, or with a positive blood pregnancy test;
  • Plan to move before the end of the trial or leave the local area for a long time during the scheduled trial visit;
  • Abnormal blood routine, blood biochemistry, urine routine, electrocardiogram or heart disease related indicators before vaccination (except for minor abnormalities judged by doctors to have no clinical significance);
  • Researchers believe that any situation that may affect the evaluation of the experiment;
  • Active tuberculosis patients;
  • For HIV infected individuals, the plasma HIV-1 RNA level during screening should be ≥ 200 copies/mL, or positive test for hepatitis B, hepatitis C and syphilis;
  • For healthy people and men and women who have sex with each other, hepatitis B, hepatitis C, syphilis and HIV) are positive;
  • Individuals who experience severe allergic reactions after the first dose of vaccination;
  • Serious adverse events that are definitely related to the first dose of vaccination;
  • For those who are newly discovered or occur after the first dose of vaccination and do not meet the inclusion criteria or the exclusion criteria for the first dose, the researcher shall determine whether to continue participating in the trial.
04

Study design

Phase
Phase 1
Primary purpose
Prevention
Allocation
Randomized
Intervention model
Parallel assignment
Masking
Quadruple (Participant, Care provider, Investigator, Outcomes assessor)
Enrollment
120 participants (estimated)

Study arms

  • Experimental
    Experimental group 1

    Participants without a history of immunization against smallpox

    Biological: MVA strain monkeypox attenuated live vaccine (low dose)

  • Experimental
    Experimental group 2

    Participants without a history of immunization against smallpox

    Biological: MVA strain monkeypox attenuated live vaccine (high dose)

  • Experimental
    Experimental group 3

    Participants with a history of immunization against smallpox

    Biological: MVA strain monkeypox attenuated live vaccine (low dose)

  • Experimental
    Experimental group 4

    Participants with a history of immunization against smallpox

    Biological: MVA strain monkeypox attenuated live vaccine (high dose)

  • Placebo comparator
    Control group 1

    Participants without a history of immunization against smallpox

    Other: Placebo

  • Placebo comparator
    Control group 2

    Participants with a history of immunization against smallpox

    Other: Placebo

Interventions

  • BiologicalMVA strain monkeypox attenuated live vaccine (low dose)

    Low dose MVA strain monkeypox attenuated live vaccine

  • BiologicalMVA strain monkeypox attenuated live vaccine (high dose)

    High dose MVA strain monkeypox attenuated live vaccine

  • OtherPlacebo

    Vaccine excipient

05

What researchers measure

Primary outcomes

  1. Immediate adverse events

    The occurrence of any adverse events within 60 minutes after each dose of vaccine immunization.

    Time frame: 60 minutes after each dose of vaccination

  2. Solicited Adverse Events

    Adverse events defined by the protocol that occurred to the participant during 0-14 days after each dose of vaccination.

    Time frame: 14 days after each dose of vaccination

  3. Unsolicited Adverse Events

    Other adverse events that occurred among participants within 0-28/30 days after each vaccination, in addition to the solicited adverse events.

    Time frame: 28 or 30 days after each dose of vaccination

  4. Abnormal incidence rate of electrocardiogram

    Abnormal electrocardiogram after 14 days of vaccination with each dose.

    Time frame: 14 days after each dose of vaccination

  5. Serious Adverse Events (SAE)

    That is serious adverse events, any serious adverse events that occurred to the participant during the study period.

    Time frame: 12 months after the last dose

  6. Adverse Event of Special Interest (AESI)

    Adverse events that require special attention as specified in the experimental protocol.

    Time frame: 12 months after the last dose

Other outcomes

  1. Geometric Mean Titer (GMT) of neutralizing antibodies

    GMI of neutralizing antibodies against monkeypox virus in the experimental vaccine groups and the placebo group.

    Time frame: 14 days after the first dose of vaccine, before the second dose of vaccine and14 days, 28 days, 6 months, and 12 months after the second dose

  2. Seroconversion rate of neutralizing antibodies

    The neutralizing antibody seroconversion rate against monkeypox virus in the experimental vaccine groups and the placebo group.

    Time frame: 14 days after the first dose of vaccine, before the second dose of vaccine and14 days, 28 days, 6 months, and 12 months after the second dose

  3. Seropositive rate of neutralizing antibodies

    The neutralizing antibody seropositive rate against monkeypox virus in the experimental vaccine groups and the placebo group.

    Time frame: 14 days after the first dose of vaccine, before the second dose of vaccine and14 days, 28 days, 6 months, and 12 months after the second dose

  4. GMT of binding antibody

    GMI of binding antibody against monkeypox virus in the experimental vaccine groups and the placebo group.

    Time frame: 14 days after the first dose of vaccine, before the second dose of vaccine and14 days, 28 days, 6 months, and 12 months after the second dose

  5. Seroconversion rate of binding antibody

    The binding antibody seroconversion rate against monkeypox virus in the experimental vaccine groups and the placebo group.

    Time frame: 14 days after the first dose of vaccine, before the second dose of vaccine and14 days, 28 days, 6 months, and 12 months after the second dose

  6. Seropositive rate of binding antibody

    The binding antibody seropositive rate against monkeypox virus in the experimental vaccine groups and the placebo group.

    Time frame: 14 days after the first dose of vaccine, before the second dose of vaccine and14 days, 28 days, 6 months, and 12 months after the second dose

  7. Cellular immune level

    Cellular immune levels of participants after receiving the experimental vaccine or placebo.

    Time frame: 14 days after the first dose of vaccine, before the second dose of vaccine and14 days, 28 days after the second dose

06

Study locations

1 of 1 sites recruiting
  • Henan Infectious Disease Hospital
    Zhengzhou, Henan, China
    Recruiting
07

References and documents

Individual participant data

Plan to share: No

No publications or documents are linked to this record.

08

Registry details

Key details

Study ID
NCT06771479
Lead sponsor
Shanghai Institute Of Biological Products
Responsible party
Sponsor
First posted
Jan 13, 2025
Start date
Jan 22, 2025
Primary completion
Jan 30, 2026 (estimated)
Completion
May 30, 2026 (estimated)
Last update
Oct 3, 2025

Study contacts

Hao Zhou, Bachelor
Contact
zhouhao5@sinopharm.com
86-021-62800991
Li
Contact
Shuang Li, Master
principal investigator · Henan Infectious Disease Hospital
Qingxia zhao, Bachelor
principal investigator · Henan Infectious Disease Hospital

Oversight

Data monitoring committee
Yes
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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