Detection of G-quadruplex DNA Methylation in Cancer

• Healthy volunteers identified as asymptomatic during medical examination;
• Age≥42 years old;
• Voluntarily participate in this study and sign the informed consent.

Exclusion Criteria:

• History of malignancy or having achieved complete remission following tumor surgery;
• Certain autoimmune diseases (e.g., systemic lupus erythematosus, Sjögren's syndrome, antiphospholipid syndrome, rheumatoid arthritis, Crohn's disease, IgA nephropathy, myasthenia gravis, dermatomyositis, immune thrombocytopenic purpura, etc.);
• Pregnancy;
• Acute trauma (including surgical procedures), sudden onset organ failure (e.g., resulting from a car accident, fulminant hepatic failure, acute pancreatitis, severe pneumonia due to influenza virus), or diagnosis of sepsis;
• Previous receipt of allogeneic blood transfusion, transplant surgery, or allogeneic cell therapy;
• Volunteers who cannot provide informed consent.