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CompletedNCT06087640Updated Sep 2, 2026

A Clinical Study to Evaluate the Efficacy, Safety and Immunogenicity of an Adjuvanted Influenza Vaccine Compared to a Non-adjuvanted Influenza Vaccine in Adults ≥65 Years of Age

A Phase 3 interventional study of aQIV or aTIV and QIV or TIV in Influenza, Human, sponsored by Seqirus. Completed at 255 sites in 21 countries. Open to participants aged 65 Years and older, including healthy volunteers. Per ClinicalTrials.gov, last updated 2026-09-02.

Sponsored by Seqirus · Phase 3, Interventional, and Prevention

Phase
Phase 3
Study type
Interventional
Enrollment
38,769
Allocation
Randomized
Ages
65 Years and older
Sex
All
01

Study summary

This Phase 3 study is a randomized, observer-blind study of MF59-adjuvanted influenza vaccine (aQIV or aTIV) compared with a non-adjuvanted influenza vaccine (QIV or TIV) in adults ≥65 years of age. The aim of the study is to evaluate MF59-adjuvanted influenza vaccine compared with non-adjuvanted influenza vaccine in the prevention of reverse transcription-polymerase chain reaction (RT-PCR)-confirmed influenza A and/or B in subjects ≥65 years of age.

02

Conditions studied

  • Influenza, Human

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Keywords

  • Influenza
  • Vaccine
  • MF59 adjuvant
  • aQIV
  • aTIV
  • A/H1N1
  • A/H3N2
  • B/Yamagata
  • B/Victoria
03

Who can participate

Ages eligible
65 Years and older
Sexes eligible
All
Accepts healthy volunteers
Yes

Inclusion criteria

In order to participate in this study, all subjects must meet ALL of the inclusion criteria described.

  1. Adults of ≥65 years of age on the day of vaccination.
  2. Individuals who have voluntarily given written informed consent after the nature of the study has been explained according to local regulatory requirements, prior to study entry.
  3. Individuals who have the ability to comply with study procedures including follow-up.

Exclusion criteria

Exclusion Criteria:

In order to participate in this study, all subjects must not meet any of the exclusion criteria described below:

  1. Bedridden subjects (i.e. confined to bed by sickness or old age).
  2. Subjects that are incapacitated and because of that in need of a Legally Authorized Representative.
  3. Receipt of any influenza vaccine within 6 months prior to enrollment or any plan to receive influenza vaccine while participating in the study.
  4. Hypersensitivity, including allergy, to any component of vaccines whose use is foreseen in this study, or severe allergic reaction (e.g. anaphylaxis) to previous influenza vaccination.
  5. Known history of Guillain-Barré syndrome or another demyelinating disease such as encephalomyelitis and transverse myelitis.
  6. Clinical conditions representing a contra-indication to intramuscular administration of vaccines or blood draw.
  7. Abnormal function of the immune system resulting from:

    1. Clinical conditions;
    2. Systemic administration of corticosteroids (PO/IV/IM) at a dose ≥20 mg/day of prednisone (or equivalent) for more than 14 consecutive days within 90 days prior to informed consent; Topical, inhaled and intranasal corticosteroids are permitted. Intermittent use (one dose in 30 days) of intra-articular corticosteroids are also permitted;
    3. Administration of antineoplastic and immunomodulating agents or radiotherapy within 90 days prior to informed consent.
  8. Receipt of immunoglobulins or any blood products within 180 days prior to informed consent.
  9. Receipt of any investigational or non-registered product (drug or vaccine) other than the study vaccine within 30 days preceding the study vaccination, or planned use during the entire study period.
  10. Acute (severe) febrile illness.
  11. Any other clinical condition that, in the opinion of the investigator, might interfere with the results of the study or pose additional risk to the subject due to participation in the study.
  12. Study personnel or immediate family members (brother, sister, child, parent) or the spouse of study personnel.
04

Study design

Phase
Phase 3
Primary purpose
Prevention
Allocation
Randomized
Intervention model
Parallel assignment
Masking
Quadruple (Participant, Care provider, Investigator, Outcomes assessor)
Enrollment
38,769 participants (actual)

Study arms

  • Experimental
    MF59-adjuvanted influenza vaccine

    MF59-adjuvanted QIV containing 2 influenza type A strains and 2 influenza type B strains or MF59-adjuvanted TIV containing 2 influenza type A strains and 1 influenza type B strain

    Biological: aQIV or aTIV

  • Other
    Non-adjuvanted influenza vaccine

    Non-adjuvanted QIV containing 2 influenza type A strains and 2 influenza type B strains or Non-adjuvanted TIV containing 2 influenza type A strains and 1 influenza type B strain

    Biological: QIV or TIV

Interventions

  • BiologicalaQIV or aTIV

    Participants receive a 0.5-mL intramuscular dose of aQIV or aTIV on Day 1. A 0.5 mL dose of aQIV contains nominally 15 µg of hemagglutinin (HA) of each of the 2 influenza type A strains and each of the 2 influenza B strains (total of 60 µg HA). A 0.5 mL dose of aTIV contains nominally 15 µg of HA of each of the 2 influenza type A strains and of the influenza B strain (total of 45 µg HA). The strain composition of aQIV and aTIV is that recommended by the World Health Organization (WHO) for quadrivalent and trivalent influenza vaccines, respectively, contemporaneous to the timing of the study.

    Also known as: Fluad Tetra/Quadrivalent or Fluad

  • BiologicalQIV or TIV

    Participants receive a 0.5-mL intramuscular dose of the non-adjuvanted QIV or TIV on Day 1. A 0.5 mL dose of QIV contains nominally 15 μg of HA of each of the 2 influenza type A strains and each of the 2 influenza B strains (total of 60 μg HA). A 0.5 mL dose of TIV contains nominally 15 μg of HA of each of the 2 influenza type A strains and of the influenza B strain (total of 45 μg HA). The strain composition of QIV and TIV is that recommended by the WHO for quadrivalent and trivalent influenza vaccines, respectively, contemporaneous to the timing of the study.

05

What researchers measure

Primary outcomes

  1. Efficacy Endpoint: First-occurrence of RT-PCR-confirmed influenza, due to any influenza Type A and/or B virus (regardless of antigenic match), using the protocol-defined ILI definition

    ILI = influenza-like illness; RT-PCR = reverse transcription-polymerase chain reaction

    Time frame: From 14 days after vaccination (ie study day 15) and until the end of influenza season (typically end of May for Northern Hemisphere [NH] influenza season and end of November for Southern Hemisphere [SH] influenza season)

Secondary outcomes

  1. Efficacy Endpoint: First-occurrence of RT-PCR-confirmed influenza, due to any influenza Type A and/or B virus (regardless of antigenic match), using the protocol-defined ILI definition

    Time frame: From 14 days after vaccination (ie study day 15) and until the end of influenza season (typically end of May for NH influenza season and end of November for SH influenza season)

  2. Efficacy Endpoint: First-occurrence of culture-confirmed influenza, due to influenza Type A and/or B virus antigenically matched to the vaccine strains selected for the seasonal vaccine, using the protocol-defined ILI definition

    Time frame: From 14 days after vaccination (ie study day 15) and until the end of influenza season (typically end of May for NH influenza season and end of November for SH influenza season)

  3. Efficacy Endpoint: First-occurrence of culture-confirmed influenza, due to any influenza Type A and/or B virus (regardless of antigenic match), using the protocol-defined ILI definition

    Time frame: From 14 days after vaccination (ie study day 15) and until the end of influenza season (typically end of May for NH influenza season and end of November for SH influenza season)

  4. Efficacy Endpoint: First-occurrence of RT-PCR-confirmed influenza, due to any influenza Type A and/or B virus (regardless of antigenic match), using the modified CDC ILI definition

    CDC = Centers for Disease Control and Prevention

    Time frame: From 14 days after vaccination (ie study day 15) and until the end of influenza season (typically end of May for NH influenza season and end of November for SH influenza season)

  5. Efficacy Endpoint: First-occurrence of RT-PCR-confirmed influenza, due to any influenza Type A and/or B virus (regardless of antigenic match), using the WHO ILI definition

    WHO = World Health Organization

    Time frame: From 14 days after vaccination (ie study day 15) and until the end of influenza season (typically end of May for NH influenza season and end of November for SH influenza season)

  6. Efficacy Endpoint: First-occurrence of culture-confirmed influenza, due to influenza Type A and/or B virus antigenically unmatched to the vaccine strains selected for the seasonal vaccine, using the protocol-defined ILI definition

    Time frame: From 14 days after vaccination (ie study day 15) and until the end of influenza season (typically end of May for NH influenza season and end of November for SH influenza season)

  7. Immunogenicity Endpoint: Pre- and post-vaccination hemagglutination inhibition (HI) geometric mean titers (GMTs) for the A/H1N1, A/H3N2, B/Yamagata, and B/Victoria vaccine strains

    Time frame: Day 1 and Day 22

  8. Immunogenicity Endpoint: Geometric mean fold increase (GMFI, Day 22/Day1) for the A/H1N1, A/H3N2, B/Yamagata, and B/Victoria vaccine strains

    Time frame: Day 1 and Day 22

  9. Immunogenicity Endpoint: Percentage of subjects achieving seroconversion for the A/H1N1, A/H3N2, B/Yamagata, and B/Victoria vaccine strains

    Seroconversion is defined as the percentage of subjects with either a pre-vaccination titer \<1:10 and a post-vaccination titer ≥1:40 or a pre-vaccination titer ≥1:10 and a ≥4-fold increase in post-vaccination titer.

    Time frame: Day 1 and Day 22

  10. Immunogenicity Endpoint: Percentage of subjects with HI titer ≥1:40 for the A/H1N1, A/H3N2, B/Yamagata, and B/Victoria vaccine strains

    Time frame: Day 1 and Day 22

  11. Safety Endpoint: All adverse events (AEs) reported within 30 minutes after vaccination

    Time frame: Day 1

  12. Safety Endpoint: Serious adverse events (SAEs) reported during the entire study period

    Time frame: Day 1 to Day 181 or the end of the influenza season, whichever is longer

  13. Safety Endpoint: Adverse events of special interest (AESIs) reported during the entire study period

    Time frame: Day 1 to Day 181 or the end of the influenza season, whichever is longer

  14. Safety Endpoint: AEs leading to premature withdrawal from the study during the entire study period

    Time frame: Day 1 to Day 181 or the end of the influenza season, whichever is longer

06

Study locations

255 sites
  • 84046-Accel Research Sites - Birmingham
    Birmingham, Alabama 35216, United States
  • 84075-Cullman Clinical Trials
    Cullman, Alabama 35055, United States
  • 84112-DM Clinical Research - Phoenix
    Phoenix, Arizona 85012, United States
  • 84114-Scottsdale Clinical Trials
    Scottsdale, Arizona 85260, United States
  • 84045-Lynn Institute of the Ozarks
    Little Rock, Arkansas 72204, United States
  • 84070-Baptist Health Center for Clinical Research
    Little Rock, Arkansas 72205, United States
  • 84101-West Coast Research LLC
    Dublin, California 94568, United States
  • 84124-Leading Edge Research
    Encino, California 91316, United States
  • 84104-Zillan Clinical Research
    Inglewood, California 90303, United States
  • 84105-Eximia Research - CA
    La Mesa, California 91942, United States
  • 84107-Long Beach Research Institute
    Long Beach, California 90805, United States
  • 84109-Long Beach Clinical Trials
    Long Beach, California 90806, United States
  • 84115-Central Valley Research
    Modesto, California 95350, United States
  • 84120-Paradigm Research
    Redding, California 96001, United States
  • 84119-Apex Clinical Research
    San Diego, California 92120, United States
  • 84125-Lynn Institute of Denver
    Aurora, Colorado 80012, United States
  • 84110-Tekton Research
    Longmont, Colorado 80501, United States
  • 84123-Paradigm Research
    Wheat Ridge, Colorado 80033, United States
  • 84085-Clinical Research Consulting, LLC
    Milford, Connecticut 06460, United States
  • 84017-Chase Medical Research, LLC
    Waterbury, Connecticut 06708, United States
  • 84029-Imagine Research of Palm Beach County
    Boynton Beach, Florida 33435, United States
  • 84067-Innovative Research of West Florida, Inc.
    Clearwater, Florida 33756, United States
  • 84006-Nature Coast Clinical Research - Crystal River
    Crystal River, Florida 34429, United States
  • 84016-USA and International Research Inc.
    Doral, Florida 33126, United States
  • 84061-Velocity Clinical Research - New Smyrna Beach
    Edgewater, Florida 32132, United States
  • 84065-Fleming Island Center for Clinical Research
    Fleming Island, Florida 32003, United States
  • 84027-SIMEDHealth, LLC
    Gainesville, Florida 32607, United States
  • 84019-Green Leaf Clinical Trials
    Jacksonville, Florida 32258, United States
  • 84026-Health Awareness, Inc.
    Jupiter, Florida 33458, United States
  • 84071-3SYNC Research
    Lake Worth, Florida 33460, United States
  • 84083-Accel Research Sites Network - St. Petersburg-Largo
    Largo, Florida 33777, United States
  • 84012-Evolution Clinical Trials
    Miami, Florida 33122, United States
  • 84082-Global Health Research Center, Inc.
    Miami Lakes, Florida 33016, United States
  • 84081-Suncoast Research Associates, LLC
    Pembroke Pines, Florida 33024, United States
  • 84024-St. Johns Center for Clinical Research
    Saint Augustine, Florida 32086, United States
  • 84051-Precision Clinical Research
    Sunrise, Florida 33351, United States
  • 84008-Global Health Research Center, Inc. - Tampa
    Tampa, Florida 33615, United States
  • 84037-Eximia Research - GA
    Atlanta, Georgia 30315, United States
  • 84086-Agile Clinical Research Trials, LLC
    Atlanta, Georgia 30328-6124, United States
  • 84054-Centricity Research Columbus
    Columbus, Georgia 31904, United States
  • 84013-M3 Wake Research/Mount Vernon Clinical Research, LLC
    Sandy Springs, Georgia 30103, United States
  • 84069-Velocity Clinical Research - Savannah
    Savannah, Georgia 31406, United States
  • 84039-North Georgia Clinical Research
    Woodstock, Georgia 30189, United States
  • 84118-Velocity Clinical Research - Boise
    Meridian, Idaho 83642, United States
  • 84060-Great Lakes Clinical Trials LLC, dba Flourish Research
    Chicago, Illinois 60640, United States
  • 84022-DM Clinical Research - River Forest
    Melrose Park, Illinois 60160, United States
  • 84025-Velocity Clinical Research - Valparaiso
    Valparaiso, Indiana 46383, United States
  • 84042-Velocity Clinical Research - Sioux City
    Sioux City, Iowa 51106, United States
  • 84055-Velocity Clinical Research - Baton Rouge
    Baton Rouge, Louisiana 70809, United States
  • 84088-Velocity Clinical Research - Covington
    Covington, Louisiana 70433, United States
  • 84052-Velocity Clinical Research - Lafayette
    Lafayette, Louisiana 70508, United States
  • 84064-Benchmark Research
    Metairie, Louisiana 70006, United States
  • 84005-DM Clinical Research - Brookline
    Brookline, Massachusetts 02446, United States
  • 84002-ActivMed Practices and Research, LLC
    Methuen, Massachusetts 01844, United States
  • 84044-Clarkston Medical Group
    Clarkston, Michigan 48346, United States
  • 84018-Quest Research Institute
    Farmington Hills, Michigan 48334, United States
  • 84032-Bioscope Clinical Research, LLC
    Farmington Hills, Michigan 48336, United States
  • 84001-Revival Research Institute, LLC
    Southfield, Michigan 48075, United States
  • 84074-DM Clinical Research - Southfield
    Southfield, Michigan 48076, United States
  • 84023-Velocity Clinical Research - Gulfport
    Gulfport, Mississippi 39503, United States
  • 84038-Kansas City Research Institute
    Kansas City, Missouri 64131, United States
  • 84057-Sundance Clinical Research
    St Louis, Missouri 63141, United States
  • 84108-Boeson Research MSO
    Missoula, Montana 59804, United States
  • 84030-Velocity Clinical Research - Grand Island
    Grand Island, Nebraska 68803, United States
  • 84040-Velocity Clinical Research - Norfolk
    Norfolk, Nebraska 68701, United States
  • 84007-Velocity Clinical Research - Omaha
    Omaha, Nebraska 68134, United States
  • 84117-M3 Wake Research/CRCN
    Las Vegas, Nevada 89106, United States
  • 84103-Alliance for Multispecialty Research, LLC
    Las Vegas, Nevada 89119, United States
  • 84111-Vector Clinical Trials
    Las Vegas, Nevada 89128, United States
  • 84106-Las Vegas Clinical Trials
    North Las Vegas, Nevada 89030, United States
  • 84003-DM Clinical Research - New Jersey
    Jersey City, New Jersey 07306, United States
  • 84122-Albuquerque Clinical Trials
    Albuquerque, New Mexico 87102, United States
  • 84048-Velocity Clinical Research - Binghamton
    Binghamton, New York 13905, United States
  • 84077-Velocity Clinical Research - Syracuse
    East Syracuse, New York 13057, United States
  • 84014-Asheville Clinical Research
    Asheville, North Carolina 28803, United States
  • 84089-Velocity Clinical Research - Durham
    Durham, North Carolina 27701, United States
  • 84035-Monroe Biomedical Research
    Monroe, North Carolina 28112, United States
  • 84102-Eximia Research - NC
    Raleigh, North Carolina 27607, United States
  • 84020-M3 Wake Research/Raleigh Clinical Research, LLC
    Raleigh, North Carolina 27612, United States
  • 84004-TMA Headlands LLC, dba Trial Management Associates
    Wilmington, North Carolina 28403, United States
  • 84079-Progressive Medicine of the Triad, LLC
    Winston-Salem, North Carolina 27103, United States
  • 84021-Velocity Clinical Research - Cleveland
    Beachwood, Ohio 44122, United States
  • 84034-CTI Clinical Research Center
    Cincinnati, Ohio 45212, United States
  • 84063-Velocity Clinical Research - Cincinnati (Mt. Auburn)
    Cincinnati, Ohio 45219, United States
  • 84078-Tekton Research
    Yukon, Oklahoma 73099, United States
  • 84121-Velocity Clinical Research - Medford
    Medford, Oregon 97504, United States
  • 84050-3SYNC Research
    Pittsburgh, Pennsylvania 15241, United States
  • 84009-Velocity Clinical Research - Providence
    East Greenwich, Rhode Island 02818, United States
  • 84010-M3 Wake Research/Charleston Clinical Trials, LLC
    Charleston, South Carolina 29414, United States
  • 84043-Velocity Clinical Research - Gaffney
    Gaffney, South Carolina 29340, United States
  • 84056-Coastal Carolina Research Center, LLC
    North Charleston, South Carolina 29405, United States
  • 84047-Velocity Clinical Research - Spartanburg
    Spartanburg, South Carolina 29303, United States
  • 84053-Velocity Clinical Research - Union
    Union, South Carolina 29379, United States
  • 84068-WR-ClinSearch, LLC
    Chattanooga, Tennessee 37421, United States
  • 84090-Alliance for Multispecialty Research, LLC
    Knoxville, Tennessee 37909, United States
  • 84084-Clinical Research Associates, Inc.
    Nashville, Tennessee 37203, United States
  • 84072-WR-Global Medical Research, LLC
    Dallas, Texas 75224, United States
  • 84028-Benchmark Research
    Fort Worth, Texas 76135, United States
  • 84015-Trio Clinical Trials LLC
    Houston, Texas 77008, United States
  • 84062-Activian Clinical Research
    Kingwood, Texas 77339, United States

Showing the first 100 of 255 sites across 21 countries.

07

References and documents

Individual participant data

Plan to share: Yes — Seqirus will consider on a case-by-case basis requests to share Individual Patient Data (IPD) with external bona-fide, qualified scientific and medical researchers. For information on the process and requirements for submitting a voluntary data sharing request for IPD, please contact Seqirus at seqirus.clinicaltrials@seqirus.com.

Supporting information: Study protocol, Sap

No publications or documents are linked to this record.

08

Registry details

Key details

Study ID
NCT06087640
Lead sponsor
Seqirus
Responsible party
Sponsor
First posted
Oct 18, 2023
Start date
Oct 23, 2023
Primary completion
May 26, 2026
Completion
Jun 15, 2026
Last update
Sep 2, 2026

Study contacts

Clinical Program Director
study director · Seqirus

Oversight

Data monitoring committee
Yes
FDA-regulated drug
Yes
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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