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CompletedNCT05972733Updated Aug 19, 2025Results posted

Serologic Assay Validation and Proficiency Testing of HIL-214 in Adults

A Phase 2 interventional study of HIL-214 in Gastroenteritis, sponsored by HilleVax. Completed at 2 sites in United States. Open to participants aged 18 Years to 49 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2025-08-19.

Sponsored by HilleVax · Phase 2, Interventional, and Prevention

Phase
Phase 2
Study type
Interventional
Enrollment
80
Allocation
Not applicable
Ages
18 Years to 49 Years
Sex
All
01

Study summary

The purpose of this trial is to obtain serum for proficiency testing to confirm assay validity is maintained following the dosing of adults with a pediatric dose of HIL-214.

Read the detailed description

This single-arm trial serves to obtain serum for proficiency testing to confirm assay validity is maintained. Exploratory aspects of this trial include evaluating additional assays used for the assessment of immune responses to HIL-214 in peripheral-blood samples. Given the large volume of blood required, the pediatric dose will be tested in healthy adults. The main scientific rationale for the trial is to identify immune assays that can assess the generation of serum antibodies or cell-mediated immunity (CMI) specific to norovirus strains not represented in the HIL-214 vaccine (i.e. cross-reactivity).

02

Conditions studied

  • Gastroenteritis

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03

Who can participate

Ages eligible
18 Years to 49 Years
Sexes eligible
All
Accepts healthy volunteers
Yes

Inclusion criteria

  • Male or female subjects aged 18 to 49 years, inclusive.
  • Individuals who are in good health at the time of entry into the trial as determined by medical history, physical examination (including vital signs) and clinical judgment of the investigator.
  • The individual signs and dates a written, informed consent form and any required privacy authorization prior to the initiation of any trial procedures, after the nature of the trial has been explained according to regulatory requirements.
  • Individuals willing and able to comply with trial procedures and are available for the duration of follow-up.

Exclusion criteria

Exclusion Criteria:

  • Females who are pregnant or breastfeeding.
  • Gastroenteritis within 14 days before planned dosing (can warrant delay of trial vaccine administration).
  • Known hypersensitivity or allergy to any of the HIL-214 components (including excipients).
  • Known or suspected impairment/alteration of immune function, including history of any autoimmune disease or neuro-inflammatory disease.
  • Any serious chronic or progressive disease (including hepatitis B or C).
  • Previous exposure to an experimental norovirus vaccine.
  • Subject or subject's first-degree relatives are involved in the trial conduct.
04

Study design

Phase
Phase 2
Primary purpose
Prevention
Allocation
Not applicable
Intervention model
Single group
Masking
None (open label)
Enrollment
80 participants (actual)

Study arms

  • Experimental
    Open Arm Study

    One dose given to all participants

    Biological: HIL-214

Interventions

  • BiologicalHIL-214

    HIL-214

05

What researchers measure

Primary outcomes

  1. Primary Immunogenicity for Panel Formation

    Serum samples were obtained for validation of the norovirus GI.1 and GII.4c histoblood group antigen (HBGA)-blocking and total immunoglobulin (pan-Ig) assays. The percentage of participants with a predefined seroresponse (≥4-fold rise in antibody concentration from Day 1 to Day 29) to the GI.1 and GII.4c components of HIL-214 and 95% confidence interval are reported.

    Time frame: Day 1 to Day 29

Secondary outcomes

  1. Adverse Events Leading to Participant Withdrawal From the Trial

    Adverse events leading to trial withdrawal were collected throughout the trial.

    Time frame: Day 1 to Day 85

  2. Percentage of Participants With Solicited Local (Injection Site) Adverse Events Within 7 Days of Vaccine Administration

    Assessed solicited local AEs included pain at injection site, redness, swelling, and induration. See AE tables for specifics.

    Time frame: Day 1 to Day 7

  3. Percentage of Participants With Solicited Systemic Adverse Events (AEs) Within 7 Days of Vaccine Administration

    Assessed solicited systemic AEs included headache, fatigue, myalgia, arthralgia, vomiting, diarrhea, and fever.

    Time frame: Day 1 to Day 7

  4. Percentage of Participants With at Least One Unsolicited AEs After the Dose of Trial Vaccine

    Unsolicited AEs are any local or systemic AEs, as defined by this study, that are not solicited.

    Time frame: Day 1 to Day 28

06

Results

Posted Aug 19, 2025

Participant flow

Participants took part in the trial at 2 investigative sites in the United States from 01 August 2023 to 10 November 2023.

Participant flow — Overall Study
MilestoneHIL-214
Started80
Completed77
Not completed3
Withdrew: Lost to follow-up2
Withdrew: Withdrawal by subject1

Outcome measures

PrimaryPrimary Immunogenicity for Panel Formation

Serum samples were obtained for validation of the norovirus GI.1 and GII.4c histoblood group antigen (HBGA)-blocking and total immunoglobulin (pan-Ig) assays. The percentage of participants with a predefined seroresponse (≥4-fold rise in antibody concentration from Day 1 to Day 29) to the GI.1 and GII.4c components of HIL-214 and 95% confidence interval are reported.

Time frame:
Day 1 to Day 29
Reported as:
Number · Percentage of Participants
Primary Immunogenicity for Panel Formation
Percentage of ParticipantsHIL-214
Anti-GI.1 HBGA-blocking seroresponse rate87.0 (77.4 to 93.6)
Anti-GII.4c HBGA-blocking seroresponse rate72.7 (61.4 to 82.3)
Anti-GI.1 and GII.4c HBGA-blocking seroresponse rate60.5 (48.6 to 71.6)
Anti-GI.1 pan-Ig seroresponse rate98.7 (93.1 to 100)
Anti-GII.4c pan-Ig seroresponse rate91.0 (82.4 to 96.3)
Anti-GI.1 and GII.4c pan-Ig seroresponse rate91.0 (82.4 to 96.3)
SecondaryAdverse Events Leading to Participant Withdrawal From the Trial

Adverse events leading to trial withdrawal were collected throughout the trial.

Time frame:
Day 1 to Day 85
Reported as:
Count of participants · Participants
Adverse Events Leading to Participant Withdrawal From the Trial
ParticipantsHIL-214
Adverse Events Leading to Participant Withdrawal From the Trial0
SecondaryPercentage of Participants With Solicited Local (Injection Site) Adverse Events Within 7 Days of Vaccine Administration

Assessed solicited local AEs included pain at injection site, redness, swelling, and induration. See AE tables for specifics.

Time frame:
Day 1 to Day 7
Reported as:
Number · percentage of participants
Percentage of Participants With Solicited Local (Injection Site) Adverse Events Within 7 Days of Vaccine Administration
percentage of participantsHIL-214
Percentage of Participants With Solicited Local (Injection Site) Adverse Events Within 7 Days of Vaccine Administration48.1
SecondaryPercentage of Participants With Solicited Systemic Adverse Events (AEs) Within 7 Days of Vaccine Administration

Assessed solicited systemic AEs included headache, fatigue, myalgia, arthralgia, vomiting, diarrhea, and fever.

Time frame:
Day 1 to Day 7
Reported as:
Number · percentage of participants
Percentage of Participants With Solicited Systemic Adverse Events (AEs) Within 7 Days of Vaccine Administration
percentage of participantsHIL-214
Percentage of Participants With Solicited Systemic Adverse Events (AEs) Within 7 Days of Vaccine Administration55.7
SecondaryPercentage of Participants With at Least One Unsolicited AEs After the Dose of Trial Vaccine

Unsolicited AEs are any local or systemic AEs, as defined by this study, that are not solicited.

Time frame:
Day 1 to Day 28
Reported as:
Count of participants · Participants
Percentage of Participants With at Least One Unsolicited AEs After the Dose of Trial Vaccine
ParticipantsHIL-214
Percentage of Participants With at Least One Unsolicited AEs After the Dose of Trial Vaccine7

Adverse events

Collected over Serious adverse events, all-cause mortality and other significant adverse events were assessed for each participant from Day 1 to Day 85. Non-serious events are listed at a 2% frequency threshold.

Adverse event summary by group
GroupDeathsSeriousOther
HIL-2140/80 (0%)0/80 (0%)52/79 (65.8%)
Most frequent other events
Most frequent other events
EventHIL-214
Injection site painInjury, poisoning and procedural complications35/79
MyalgiaGeneral disorders31/79
FatigueGeneral disorders27/79
HeadacheGeneral disorders24/79
ArthralgiaGeneral disorders11/79
IndurationInjury, poisoning and procedural complications7/79
DiarrheaGeneral disorders7/79
RednessInjury, poisoning and procedural complications6/79
SwellingInjury, poisoning and procedural complications4/79
VomitingGeneral disorders4/79

Baseline characteristics

Safety Analysis Set, all participants that received trial vaccine (HIL-214).

Age, Categorical
Age, Categorical(Participants)HIL-214
<=18 years0
Between 18 and 65 years80
>=65 years0
Age, Continuous
Age, Continuous(years)HIL-214
Mean35.2 ± 9.21
Sex: Female, Male
Sex: Female, Male(Participants)HIL-214
Female48
Male32
Ethnicity (NIH/OMB)
Ethnicity (NIH/OMB)(Participants)HIL-214
Hispanic or Latino17
Not Hispanic or Latino63
Unknown or Not Reported0
Race (NIH/OMB)
Race (NIH/OMB)(Participants)HIL-214
American Indian or Alaska Native1
Asian5
Native Hawaiian or Other Pacific Islander0
Black or African American40
White31
More than one race2
Unknown or Not Reported1
Region of Enrollment
Region of Enrollment(participants)HIL-214
United States80
Weight
Weight(kg)HIL-214
Mean87.32 ± 24.573
Height
Height(cm)HIL-214
Mean170.70 ± 10.822

1 further baseline measures are reported on the registry.

07

Study locations

2 sites
  • Pennisula Research Associates
    Rolling Hills Estates, California 90274, United States
  • Clinical Research Atlanta
    Stockbridge, Georgia 30281, United States
08

References and documents

Study documents

  • Study protocol · Oct 25, 2023
  • Statistical analysis plan · Jan 12, 2024

Documents are hosted by the registry — open the source record to download them.

09

Registry details

Key details

Study ID
NCT05972733
Lead sponsor
HilleVax
Responsible party
Sponsor
First posted
Aug 2, 2023
Start date
Aug 1, 2023
Primary completion
Aug 14, 2023
Completion
Nov 10, 2023
Results posted
Aug 19, 2025
Last update
Aug 19, 2025

Oversight

Data monitoring committee
No
FDA-regulated drug
Yes
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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