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CompletedNCT05727774THERMIDORUpdated Jun 12, 2026

Effect of Water Biking in Hot Versus Neutral Water for Heat Acclimatation

An interventional study of Water Biking in hot water condition and Water Biking in neutral water condition in Healthy and Heat Exposure, sponsored by University Hospital, Montpellier. Completed at 1 site in France. Open to participants aged 18 Years to 60 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2026-06-12.

Sponsored by University Hospital, Montpellier · Not applicable, Interventional, and Prevention

Phase
Not applicable
Study type
Interventional
Enrollment
24
Allocation
Randomized
Ages
18 Years to 60 Years
Sex
All
01

Study summary

The goal of this clinical trial is to compare the efficiency of water biking training in hot (35°C) versus neutral (25°C) temperature water to elicite heat acclimation in healthy recreative athletes.

Read the detailed description

The goal of this clinical trial is to compare the efficiency of water biking training in hot (35°C) versus neutral (25°C) temperature water to elicite heat acclimation in healthy recreative athletes. The main questions it aims to answer are:

  • Does the water biking training protocol in hot versus neutral temperature water improve the time trial in an artificially hot and humid indoor environment?
  • Does the water biking training protocol in hot versus neutral temperature water improve the VO2 max performance and force/velocity profile in neutral indoor environment?
  • What are the main acclimation adaptations (central and cutaneous temperature), heart rate, cardiac function, sweating quality, sleep quality, haematologic profile.

Participants will have to do pre and post training tests in both normal environment (cardiorespiratory stress test, force/velocity profile test, echocardiography) and artificially hot and humid indoor environment (time trial). The training protocols consists in 10 sessions of 1 hour of water bike aerobic training (5 times a week for 2 weeks) at an intensity based on the heart rate corresponding to the 1st respiratory threshold, in hot (interventional group IG: 35°C) versus neutral (control group CG: 25°C) water.

02

Conditions studied

  • Healthy
  • Heat Exposure

Keywords

  • Water biking
  • Acclimatization
03

In context

Lead sponsor

University Hospital, Montpellier is the lead sponsor of 1,244 studies on the registry; 225 are open to participants now.

Counted across the registry records on this site, refreshed daily.

04

Who can participate

Ages eligible
18 Years to 60 Years
Sexes eligible
All
Accepts healthy volunteers
Yes

Inclusion criteria

  • Healthy people
  • Sports practicing

Exclusion criteria

Exclusion Criteria:

  • Acclimatation already done (recent training in hot and/or humid) less than 2 months
  • Heart disease recent or non controlled
  • Heat intolerance
  • Water phobia or inable to swim
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Study design

Phase
Not applicable
Primary purpose
Prevention
Allocation
Randomized
Intervention model
Parallel assignment
Masking
None (open label)
Enrollment
24 participants (actual)

Study arms

  • Experimental
    Hot water condition

    Participants do the water biking training for two weeks, 1 hour a day, in hot water temperature (35°C)

    Other: Water Biking in hot water condition

  • Sham comparator
    Neutral water condition

    Participants do the water biking training for two weeks, 1 hour a day, in neutral water temperature (25°C)

    Other: Water Biking in neutral water condition

Interventions

  • OtherWater Biking in hot water condition

    Training protocol includes 10 water biking sessions in hot water temperature (35°C) for this group

  • OtherWater Biking in neutral water condition

    Training protocol includes 10 water biking sessions in neutral water temperature (25°C) for this group

06

What researchers measure

Primary outcomes

  1. Change of the distance traveled (meter) on a Time Trial between pre and post training tests in hot and humid atmosphere (environmental room)

    The participant has to perform the longer distance possible in 30 min on a stationary bike in hot and humid atmosphere (environmental room). The change of distance (measured in meter) will be assessed before and after the 2 weeks training program.

    Time frame: The 1st Time Trial will be performed the week preceding the program training. The 2nd Time Trial will be performed the week following the program training

Secondary outcomes

  1. Change in VO2 Max (ml/kg/min) between pre and post training tests in normal environment

    The participant has to perform a maximal cardiorespiratory test on a stationary bike in normal environment. The change in VO2 Max (measured in ml/kg/min) will be assessed before and after the 2 weeks training program.

    Time frame: The 1st maximal cardiorespiratory test will be performed the week preceding the program training. The 2nd maximal cardiorespiratory test will be performed the week following the program training

  2. Change in Maximal Aerobic Power (Watts) between pre and post training tests in normal environment

    The participant has to perform a maximal cardiorespiratory test on a stationary bike in normal environment. The change in Maximal Aerobic Power (measured in Watts) will be assessed before and after the 2 weeks training program.

    Time frame: The 1st maximal cardiorespiratory test will be performed the week preceding the program training. The 2nd maximal cardiorespiratory test will be performed the week following the program training

  3. Change of "global longitudinal strain of left ventricule" (%) measured during an echocardiography between pre and post training tests in normal environment

    The participant has to pass an echocardiography with specific measurment of "global longitudinal strain of left ventricule" (united in %). The change will be assessed before and after the 2 weeks training program.

    Time frame: The 1st echocardiography will be performed the week preceding the program training. The 2nd echocardiography will be performed the week following the program training

  4. Change on central temperature (°C) between pre and post training test in hot and humid atmosphere (environmental room)

    Central temperature will be measured during Time Trial in hot and humid atmosphere (environmental room). The change on central temperature (measured in °C) will be assessed before and after the 2 weeks training program.

    Time frame: The 1st Time Trial will be performed the week preceding the program training. The 2nd Time Trial will be performed the week following the program training

  5. Change on cutaneours temperature (°C) between pre and post training test in hot and humid atmosphere (environmental room)

    Cutaneous temperature will be measured during Time Trial in hot and humid atmosphere (environmental room). The change on cutaneours temperature (measured in °C) will be assessed before and after the 2 weeks training program.

    Time frame: The 1st Time Trial will be performed the week preceding the program training. The 2nd Time Trial will be performed the week following the program training

  6. Change of Na concentration (mmol/L) in the sweat between pre and post training test in hot and humid atmosphere (environmental room)

    Sweat quality will be measured during Time Trial in hot and humid atmosphere (environmental room). The change of Na concentration (measured in mmol/l) will be assessed before and after the 2 weeks training program.

    Time frame: The 1st Time Trial will be performed the week preceding the program training. The 2nd Time Trial will be performed the week following the program training

  7. Change of body weight loss (Kg) between pre and post training test in hot and humid atmosphere (environmental room)

    Body weight loss will be measured before and after Time Trial in hot and humid atmosphere (environmental room). The change of the body weight (measured in Kg) will be assessed between before and after the 2 weeks training program.

    Time frame: The 1st measure of body weight will be performed the week preceding the program training. The 2nd measure of body weight will be performed the week following the program training

  8. Change of Sleep quality between the first and the last training session of water biking

    Sleep quality will be measured after the first and the last training session using a specific sleep self-questionnaire (PSQI) to detect the effect of hot or neutral water training during the 2 weeks training program. PSQI : Pittsburgh Sleep Quality Index. Qualitative questionnaire, no score measured.

    Time frame: The sleep self-questionnaire will be performed immediately after the first training session (Day 1) and immediately after the last training session (Day 10) of water biking

  9. Change of force/velocity power (N/m) between pre and post training test

    The participant has to perform a maximal force/velocity test to measure the force/velocity power on a specific fitness testing cycle (Monark 818 E). The change of force/velocity power (measured in N/m) will be assessed between before and after the 2 weeks training program.

    Time frame: The 1st measure of force/velocity power will be performed the week preceding the program training. The 2nd measure of force/velocity power will be performed the week following the program training

  10. Change of sytemic low grade inflammation (CRP = C-Reactive Protein, mg/l) between pre and post training test

    A blood test will be performed to measure the change of CRP concentration (measured in mg/l) between before and after the 2 weeks training program.

    Time frame: The 1st measure of CRP will be performed the week preceding the program training. The 2nd measure of CRP will be performed the week following the program training

  11. Change of haematocrit (%) between pre and post training test

    A blood test with complete blood count will be performed to measure the change of haematocrit (which is expressed in %) between before and after the 2 weeks training program.

    Time frame: The 1st measure of haematocrit will be performed the week preceding the program training. The 2nd measure of haematocrit will be performed the week following the program training

  12. Change of hemoglobin level (g/100ml) between pre and post training test

    A blood test with complete blood count will be performed to measure the change of hemoglobin level (measured in g/100ml) between before and after the 2 weeks training program.

    Time frame: The 1st measure of hemoglobin level will be performed the week preceding the program training. The 2nd measure of hemoglobin level will be performed the week following the program training

  13. Change of Rate of Perceived Effort (RPE) on a Time Trial between pre and post training tests

    The Borg Rating of Perceived Exertion (RPE) will be used to measure physical activity intensity level. The scale is from 6 to 20. The higher the score is, the higher the perception of effort is. RPE will be measured after the first Time Trial and after the second Time Trial (post training protocol).

    Time frame: The 1st Time Trial will be performed the week preceding the program training. The 2nd Time Trial will be performed the week following the program training

07

Study locations

1 site
  • Département de Médecine Physique et Réadaptation
    Montpellier, 34295, France
08

References and documents

Publications

  • Periard JD, Travers GJS, Racinais S, Sawka MN. Cardiovascular adaptations supporting human exercise-heat acclimation. Auton Neurosci. 2016 Apr;196:52-62. doi: 10.1016/j.autneu.2016.02.002. Epub 2016 Feb 12. PubMed 26905458 ↗
  • Kelly M, Gastin PB, Dwyer DB, Sostaric S, Snow RJ. Short Duration Heat Acclimation in Australian Football Players. J Sports Sci Med. 2016 Feb 23;15(1):118-25. eCollection 2016 Mar. PubMed 26957934 ↗
  • Zurawlew MJ, Walsh NP, Fortes MB, Potter C. Post-exercise hot water immersion induces heat acclimation and improves endurance exercise performance in the heat. Scand J Med Sci Sports. 2016 Jul;26(7):745-54. doi: 10.1111/sms.12638. Epub 2015 Dec 9. PubMed 26661992 ↗
  • Zurawlew MJ, Mee JA, Walsh NP. Post-exercise Hot Water Immersion Elicits Heat Acclimation Adaptations in Endurance Trained and Recreationally Active Individuals. Front Physiol. 2018 Dec 18;9:1824. doi: 10.3389/fphys.2018.01824. eCollection 2018. PubMed 30618833 ↗
  • Racinais S, Alonso JM, Coutts AJ, Flouris AD, Girard O, Gonzalez-Alonso J, Hausswirth C, Jay O, Lee JK, Mitchell N, Nassis GP, Nybo L, Pluim BM, Roelands B, Sawka MN, Wingo J, Periard JD. Consensus recommendations on training and competing in the heat. Br J Sports Med. 2015 Sep;49(18):1164-73. doi: 10.1136/bjsports-2015-094915. Epub 2015 Jun 11. PubMed 26069301 ↗
  • Zurawlew MJ, Mee JA, Walsh NP. Post-exercise Hot Water Immersion Elicits Heat Acclimation Adaptations That Are Retained for at Least Two Weeks. Front Physiol. 2019 Aug 28;10:1080. doi: 10.3389/fphys.2019.01080. eCollection 2019. PubMed 31555140 ↗
  • Helmer G, Laurent M, Rubio J, Duflos C, Hayot M, Myzia J, Hedon C, Gouzi F, Candau R, Racinais S, Julia M. Training in hot water immersion improved exercise performance in hot and humid conditions in recreational athletes - a randomized controlled trial. Eur J Appl Physiol. 2026 Jan;126(1):327-336. doi: 10.1007/s00421-025-05876-1. Epub 2025 Jul 15. PubMed 40663144 ↗
09

Updates

Tracking since Sep 25, 2026
No changes since tracking began. The registry record was last updated on Jun 12, 2026, before this site started recording changes on Sep 25, 2026. Its history is on ClinicalTrials.gov ↗
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Registry details

Key details

Study ID
NCT05727774
Lead sponsor
University Hospital, Montpellier
Responsible party
Sponsor
First posted
Feb 14, 2023
Start date
Feb 8, 2023
Primary completion
Mar 10, 2023
Completion
Mar 10, 2023
Last update
Jun 12, 2026

Oversight

Data monitoring committee
No
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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